Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Tsatsenko Lyudmila Vladimirovna

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

Web site url

Email

t-lemna@yandex.ru


Articles count: 73

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2252 kb

ICONOGRAPHY OF WHEAT

abstract 1331709021 issue 133 pp. 240 – 262 30.11.2017 ru 623
The study of agronomy plants history, its distribution and use through image analysis and iconography have been presented. As an object of study, the image of wheat has been taken as the ancient culture of the world among agricultural plants. After many years, the illustrative series takes on a completely different meaning, namely, it becomes an independent resource of information. The analysis includes images of wheat in painting, sculpture, mosaic, tapestries, murals, botanical illustrations, stamps, porcelain and scientific drawings. In the work, we have made an attempt to analyze the images of wheat in the works of art in order to search for species diversity, directions of selection and genetic research, and also to present the morphological characteristics of culture. The method of analysis is a method of visual notes or sketches, which consists in comparing and searching information by image. The analysis is carried out on grounds: tall wheat, multiflorous forms of wheat, nonspreading, thickness of straw. The visual analysis of the images of wheat in works of art by the method of sketches made it possible to reveal the species diversity of culture along the ear, to see the ancient forms that were cultivated in the world before the "green revolution". The analysis included works of art from the 14th to the 21st century, the masters of painting in Italy, Germany, Russia, the Netherlands, France, the United States, and others
674 kb

METHODOLOGICAL APPROACHES TO THE FORMATION OF THE DATABASE BASED ON THE ICONOGRAPHY OF PLANTS

abstract 1341710046 issue 134 pp. 570 – 584 29.12.2017 ru 879
The article considers methodological approaches to creating a database of the agricultural crops iconography. At present time, involving in the educational and scientific process the artistic works, as a resource of visual information, becomes a very actual and even indispensable tool for the formation of new knowledge about cultivated plants. The work deals with iconography as a system of images of certain subjects and characters presented in painting, ceramics, mosaic, sculpture, tapestry, murals, stamps, postcards, posters, labels, photos giving information about this or that agricultural plant. Electronic databases are the kind of information portal with visual images. Databases for the iconography of agricultural plants are successfully used in the scientific process, because exist the e evidence of changes in plants under the influence of scientific selection, the search for rare and ancient genotypes. Any source of information contains only a certain set of information, far from exhausting existing points of view, statistical data, analytical calculations relating directly or indirectly to this topic. Due to this circumstance, there is a need to search for new forms of information that can be successfully implemented in the iconography of the image of agricultural plants. The creation of electronic databases includes several stages. Development of the database structure. If this is a separate topic, then the basic questions of the topic are revealed and saturated with visual images. Selection of visual images. As visual images we can consider paintings of artists, cartoons, drawings, schemes, infographics, i.e. illustrations of various kinds
159 kb

MULTIFLOROUS FORMS OF BREAD WINTER WHEAT AS A MODEL OBJECT IN THE RE-SEARCH OF THE REPRODUCTIVE POTENTIAL OF THE MAIN EAR

abstract 1401806024 issue 140 pp. 100 – 111 29.06.2018 ru 384
The article deals with the realization of the re-productive potential of wheat with the use of multi-flower formation as a model object of winter soft wheat plants. The essence of the concept of "reproductive potential" is disclosed, as well as the significance of this concept for research aimed at increasing the productivity of wheat plants. The data obtained during the study and analysis of varieties of winter soft wheat with the sign of multi-flower formation are given. The reproductive potential of winter soft wheat plants was determined by counting flowers at the VI stage of organogenesis. The examined multi-flower forms of soft winter wheat compared with Krasnodar breeding varieties showed increased reproductive potential, as well as an increased number of grains with the ear, but with a reduced mass of 1000 grains. According to the data obtained, the degree of the reproductive potential of multi-flower forms of winter soft wheat varied significantly, depending on the weather and climate conditions of the year of the study. In conclusion, the choice of multi-flower formation of winter soft wheat as a model object for investigating the reproductive potential of wheat, as well as the factors that influence its implementation and the extent of such influence is justified
629 kb

GIANT FRUITS OF PLANTS AS MODEL OBJECTS IN BREEDING RESEARCHES

abstract 1411807014 issue 141 pp. 65 – 76 28.09.2018 ru 369
The article considers phenomenon of the formation of giant fruits in higher plants. As regards the size of plants, several positions are distinguished such as giant fruit, in a common habit, i.e. the size of the whole plant; depending on the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market nowadays
1294 kb

GIANT FRUIT – THE ICONOGRAPHY AND THE HISTORY OF THE ISSUE

abstract 1421808015 issue 142 pp. 26 – 40 31.10.2018 ru 384
The article considers the phenomenon of the formation of giant fruits in higher plants. As for the size of plants, several positions are distinguished such as giant fruit, a common habit, i.e. the size of the whole plant; or the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market today
182 kb

STUDY OF THE COLLECTION OF HYBRIDS OF TOMATO F1 WITH RESISTANCE TO POWDERY MILDEW FOR GROWING IN WINTER GLASS GREENHOUSES

abstract 1501906016 issue 150 pp. 143 – 155 28.06.2019 ru 398
Modern domestic tomato hybrid should be competitive with tomato hybrids of foreign selection. To create a model of a future hybrid, a collection of modern tomato hybrids of domestic and foreign selection presented on the seed market was investigated. During the study, a modern method of genetic study of the genotype of tomato Real-Time PCR was used. As a result of the study, a model of a modern tomato hybrid with tolerance to powdery mildew of tomato was described. In the future, it is planned to carry out breeding work to create such a hybrid. In the course of studying the F1 collection of tomato hybrids, it was found that the modern model of a tomato hybrid should have the following characteristics: a semi-vegetative type of growth with closely spaced internodes, early and medium ripening, a simple type of inflorescence with 4–5 fruits if the average weight of fruit is more than 200 grams; with 6–7 fruits weighing up to 150 grams for collection by tassels. High strength of fruits with the absence of concentric and radial cracking, suitable for long-distance transportation, is required. In the genotype of the modern hybrid model, in addition to tomato powdery mildew resistance (Oidiun lycopersici), it is necessary to have a complex of resistance genes to such diseases as tobacco mosaic virus (Tomato mosaic tobamovirus), Fusarium oxysporum, verticillosis wilt of the tomato; ), brown spot of tomato (Cladosporium fulvum). It is also desirable the presence of a gene of resistance to the yellow leaf curl virus of tomato (Tomato yellow leaf curl virus) and the gall nematode (Meloidogyne incognita)
366 kb

SPIKE PRODUCTIVITY IMPLEMENTATION IN COLLECTION SAMPLES OF WINTER SOFT WHEAT

abstract 1572003021 issue 157 pp. 282 – 292 31.03.2020 ru 172
The work is devoted to studying the productivity of an ear of winter soft wheat samples from the collection of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov (VIR). The study attempted to conduct a pre-selection study of collection material on the elements of spike productivity. The concept of pre-selection research is based on the study of initial collection material according to given characteristics in order to identify the best genotypes for further use in breeding practice. In the analysis of the collection material, the following characteristics were used as indicators of spike productivity: length of the main spike (cm), number of spikelets in the main spike (pcs), number of grains in the main spike (pcs), grain weight from the main spike (g), 1000-kernel weight (g). Plant height was one of the basic indicators of the studied material, because a number of samples differed in alleles of short-stem genes. According to the results of two years of research, according to the indicator «1000-kernel weight», the varietal sample Cologna lunga razza 188 (Italy) was identified as 71,20 g. According to the indicator «number of grains in an ear», the varietal sample Fenotipo 1 (Italy) was 57,3 g. Also according to the totality of indicators, the varietal sample Kaloyan (Bulgaria) with an average of two years of research with a 1000-kernel weight – 51,77 g, the number of grains in an ear – 51,6 pcs. and a mass of grains per ear – 2,71 g.
231 kb

USING REAL-TIME PCR HYBRIDIZATION PROBES, ON THE EXAMPLE, OF A MARKER OF THE TMV RESISTANCE GENE IN A TOMATO CULTURE

abstract 1582004012 issue 158 pp. 168 – 180 30.04.2020 ru 224
The article discusses the method of selection using markers in the selection of parent pairs for hybridization, in the selection process and in the subsequent evaluation of parent lines, used in linear and backcross selection of tomatoes. The work was performed on tomato hybrids that combine resistance genes to tomato mosaic virus (ToMV) or tobacco mosaic virus (TMV) in their genome. The research used seeds of foreign F1 tomato hybrids recommended by the manufacturer as resistant to the tobacco mosaic virus. Most of the tomato hybrids were Dutch selection, such leading firms as De Ruiter Seeds, Enza Zaden, Rijk Zwaan. The aim of the work was to test a system of hybridization probes designed to identify a gene for resistance to the tobacco mosaic virus. The study of the genotype of tomato plants was carried out in the laboratory of molecular diagnostics of plants , equipped with modern devices for PCR analysis on the basis of NIIOZG (Krymsk). Plant material was collected in different phases of plant vegetation. pre-numbered 1.5 ml test tubes (SSI-1200-00), plastic tablets, tweezers, and distilled water were used to collect plant samples. The collected samples were stored in a refrigerator until the plant DNA was isolated. During the study, collectible tomato samples were studied, including 8 F1 hybrids and 2 lines used as controls. According to the results of genetic analysis using the marker of the TMV resistance gene, it was found that all hybrids and one control line # 175/14 have a resistance gene in their genotype, and the analysis also showed the state of the gene – homozygous or heterozygous. The results of artificial infection confirmed the results obtained by PCR analysis. At the same time, using genetic research, it is possible to determine the exact state of the gene in the plant, which cannot be determined by methods of artificial infection, since no visual differences were found between plants with a heterozygous and homozygous state of the gene. The created marker and PCR analysis method can be recommended for wide application in the selection process of tomato culture. As a result of research, it was found that modern tomato hybrids have a gene for resistance to tobacco mosaic virus (TMV) in their genotype. This was determined by genetic analysis and confirmed by a test using artificial infection
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