Name
Tsatsenko Lyudmila Vladimirovna
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
Articles count: 73
The article is devoted to visual analysis and its application in the selection process. New possibilities of application of visual display of information in the research process are considered. We have presented the examples of the use of visual phenotyping in breeding, evaluated the advantages and disadvantages in this area. In particular, the algorithm of the program of LHDetect for estimating leaf pubescence of wheat wafers is described, based on the analysis of digital images of the sheet bend. We have also considered Phenotiki platform that uses digital images of the profile of plants for visual phenotyping to assess plant growth. Based on the analysis of various studies it was noted that the application of visual analysis for some purposes is very effective, but for others, on the contrary, not effective. We highlight the actual problems of proper representation and converting information. The article shows the various causes of loss and distortion of data, as well as possible solutions to these problems. The work describes the application of ASK-analysis in the selection process. As an example, it describes the evaluation of the polymorphism of variety groups of grapes. We have presented interesting solutions and ways to minimize distortion and loss of information in the process of its transformation found by the authors of these works. The article reveals the concept of "computational biology" and describes fundamental differences from other areas as well as tasks, problems, possible research areas and promising directions of development of computer biology
The article discusses the effect of additional spikelets
in the variety of Bezostaya 1 on the realization of
productivity of the examined plants. We traced the
frequency of occurrence of multirov spikes and studied
the effect of this phenomenon on the productivity and
the main quantitative characteristics of the studied
plants. The article demonstrates the structure
distinction of productivity for abnormal and normal
ears. Experience was one-way vegetation, planted in
triplicate, time of experiment - 2013 - 2015 years.
The study revealed that the most frequent was the
emergence of 1-4 additional spikelets per spike. Some
quantitative characters had a slight deviation, these
include the number of spiked ledges on the kernek,
spike length and plant height. To a large extent we
increased the characteristic values such as the number
of grains per ear, number of spikelets per spike,
number of grains onto the ledge, head weight and the
weight of grains spike. We significantly reduced
weight of 1000 grains. It was revealed that more
localized spikes were mostly in the middle, the most
productive part of the ear. As a result, changes have
affected not only the quantitative characteristics
related to the productivity of the ear, but also its
architectonic whole
The article deals with the realization of the re-productive potential of wheat with the use of multi-flower formation as a model object of winter soft wheat plants. The essence of the concept of "reproductive potential" is disclosed, as well as the significance of this concept for research aimed at increasing the productivity of wheat plants. The data obtained during the study and analysis of varieties of winter soft wheat with the sign of multi-flower formation are given. The reproductive potential of winter soft wheat plants was determined by counting flowers at the VI stage of organogenesis. The examined multi-flower forms of soft winter wheat compared with Krasnodar breeding varieties showed increased reproductive potential, as well as an increased number of grains with the ear, but with a reduced mass of 1000 grains. According to the data obtained, the degree of the reproductive potential of multi-flower forms of winter soft wheat varied significantly, depending on the weather and climate conditions of the year of the study. In conclusion, the choice of multi-flower formation of winter soft wheat as a model object for investigating the reproductive potential of wheat, as well as the factors that influence its implementation and the extent of such influence is justified
The article considers the phenomenon of the formation of giant fruits in higher plants. As for the size of plants, several positions are distinguished such as giant fruit, a common habit, i.e. the size of the whole plant; or the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market today
Modern domestic tomato hybrid should be competitive with tomato hybrids of foreign selection. To create a model of a future hybrid, a collection of modern tomato hybrids of domestic and foreign selection presented on the seed market was investigated. During the study, a modern method of genetic study of the genotype of tomato Real-Time PCR was used. As a result of the study, a model of a modern tomato hybrid with tolerance to powdery mildew of tomato was described. In the future, it is planned to carry out breeding work to create such a hybrid. In the course of studying the F1 collection of tomato hybrids, it was found that the modern model of a tomato hybrid should have the following characteristics: a semi-vegetative type of growth with closely spaced internodes, early and medium ripening, a simple type of inflorescence with 4–5 fruits if the average weight of fruit is more than 200 grams; with 6–7 fruits weighing up to 150 grams for collection by tassels. High strength of fruits with the absence of concentric and radial cracking, suitable for long-distance transportation, is required. In the genotype of the modern hybrid model, in addition to tomato powdery mildew resistance (Oidiun lycopersici), it is necessary to have a complex of resistance genes to such diseases as tobacco mosaic virus (Tomato mosaic tobamovirus), Fusarium oxysporum, verticillosis wilt of the tomato; ), brown spot of tomato (Cladosporium fulvum). It is also desirable the presence of a gene of resistance to the yellow leaf curl virus of tomato (Tomato yellow leaf curl virus) and the gall nematode (Meloidogyne incognita)
The article considers phenomenon of the formation of giant fruits in higher plants. As regards the size of plants, several positions are distinguished such as giant fruit, in a common habit, i.e. the size of the whole plant; depending on the harvest. Examples of different types of gigantism in plants are given. It is shown that since the ancient times the phenomenon of gigantism has been widespread. Various reasons for the appearance of abnormally large plants and fruits are considered, growth genes regulating the process of cell division and formation are given. The study of the connections between plant organs that conduct and consume the assimilant, redistribute and use carbon is easy in the concept of donor-acceptor relationships. As a model object for studying the growth of giant fruits, we have examined varieties of pumpkin called Cucurbita maxima. Thus, in a short period, a paradigm shift occurred. From surprise and enthusiasm for the gigantism of plants, the issue of cellular growth regulation, the criteria for evaluating the reproduction process, the search for links with the size, number of cells and their properties is become. New model objects have appeared, the analysis of images of plant giants has shown a unique visual resource on various objects. New knowledge about the growth of plants and the ability to regulate the process formed the basis for selection programs for obtaining vegetables with specified fruit parameters, which makes them competitive in the market nowadays
The article discusses the method of selection using markers in the selection of parent pairs for hybridization, in the selection process and in the subsequent evaluation of parent lines, used in linear and backcross selection of tomatoes. The work was performed on tomato hybrids that combine resistance genes to tomato mosaic virus (ToMV) or tobacco mosaic virus (TMV) in their genome. The research used seeds of foreign F1 tomato hybrids recommended by the manufacturer as resistant to the tobacco mosaic virus. Most of the tomato hybrids were Dutch selection, such leading firms as De Ruiter Seeds, Enza Zaden, Rijk Zwaan. The aim of the work was to test a system of hybridization probes designed to identify a gene for resistance to the tobacco mosaic virus. The study of the genotype of tomato plants was carried out in the laboratory of molecular diagnostics of plants , equipped with modern devices for PCR analysis on the basis of NIIOZG (Krymsk). Plant material was collected in different phases of plant vegetation. pre-numbered 1.5 ml test tubes (SSI-1200-00), plastic tablets, tweezers, and distilled water were used to collect plant samples. The collected samples were stored in a refrigerator until the plant DNA was isolated. During the study, collectible tomato samples were studied, including 8 F1 hybrids and 2 lines used as controls. According to the results of genetic analysis using the marker of the TMV resistance gene, it was found that all hybrids and one control line # 175/14 have a resistance gene in their genotype, and the analysis also showed the state of the gene – homozygous or heterozygous. The results of artificial infection confirmed the results obtained by PCR analysis. At the same time, using genetic research, it is possible to determine the exact state of the gene in the plant, which cannot be determined by methods of artificial infection, since no visual differences were found between plants with a heterozygous and homozygous state of the gene. The created marker and PCR analysis method can be recommended for wide application in the selection process of tomato culture. As a result of research, it was found that modern tomato hybrids have a gene for resistance to tobacco mosaic virus (TMV) in their genotype. This was determined by genetic analysis and confirmed by a test using artificial infection
The work is devoted to studying the productivity of an ear of winter soft wheat samples from the collection of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov (VIR). The study attempted to conduct a pre-selection study of collection material on the elements of spike productivity. The concept of pre-selection research is based on the study of initial collection material according to given characteristics in order to identify the best genotypes for further use in breeding practice. In the analysis of the collection material, the following characteristics were used as indicators of spike productivity: length of the main spike (cm), number of spikelets in the main spike (pcs), number of grains in the main spike (pcs), grain weight from the main spike (g), 1000-kernel weight (g). Plant height was one of the basic indicators of the studied material, because a number of samples differed in alleles of short-stem genes. According to the results of two years of research, according to the indicator «1000-kernel weight», the varietal sample Cologna lunga razza 188 (Italy) was identified as 71,20 g. According to the indicator «number of grains in an ear», the varietal sample Fenotipo 1 (Italy) was 57,3 g. Also according to the totality of indicators, the varietal sample Kaloyan (Bulgaria) with an average of two years of research with a 1000-kernel weight – 51,77 g, the number of grains in an ear – 51,6 pcs. and a mass of grains per ear – 2,71 g.