Name
Коnоnеnко Sergey Ivanovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
North-Caucasus Research Institute of Animal
Web site url
—
Articles count: 32
The isolation of E.coli phages from samples of natural
and waste water obtained during expeditions in the
different regions of Russian Federation was carried
out. The obtained phages (286 isolates) were tested for
their ability to lyse the pathogenic strains of E. coli –
pathogenic agents of pig colibacteriosis in Krasnodar
region. The study was conducted of their ability to
phage transduction, the molecular-genetic
characterization and biotechnological parameters of
selected bacteriophages. For first experimental design
of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown
that all the investigated phages are representatives of
T4-type phages of family Myoviridae. The reported
study was partially supported by RFBR, research
projects No. 08-04-99111, 09-04-10132, 16-44-
230855
Modern pig farming is a leading global livestock
industry, but the organization of adequate balanced
feeding is actual for the industry. Digestibility of
metabolizable energy of feed depends on various
factors, including the content of non-starch
polysaccharides, which have a negative effect on the
digestibility of key nutrients of the feed, lead to a
decrease in growth rate and conversion efficiency of
nutrients. Enzyme preparations which composition
generally includes Xylanase, cellulase, β-glucanase
activities, may avoid the negative impact of non-starch
polysaccharides. Today's promising preparation that
combines the functions of two feed additives - feed
enzyme and probiotic, is an enzyme preparation
Cellobacterin that, due to the particular organization of
the enzyme complex, increases the digestibility of
barley grain and effectively effects on sunflower meal.
As a probiotic preparation it inhibits the development
of pathogenic microorganisms and promotes the
formation of beneficial microflora in the digestive
tract. The introduction of Cellobacterin enzyme
preparation into the compound feed with a high
content of barley grain and sunflower meal increases
the body weight of young pigs by 4.8% and reduces
the cost of feed per 1 kg of live weight gain. The
results of hematological studies found no significant
deviations from the physiological norm in the
morphological picture of the blood in experimental
animals. The group, where Cellobacterin was added,
showed higher vitamin A content by 2.8% as
compared to the control, while vitamin E is found in
both groups on the same level. Based on the results of
the research to improve the productivity and growth
rate in compound feed based on barley and sunflower
meal with higher fiber content, it is recommended to
include in the compound feed Cellobacterin enzyme
preparation at the rate of 1 kg per 1 ton of feed
In the decision to establish solid fodder resources
in the arid parts of Southern Russia triticale should
take its rightful place. It must be introduced instead
of winter wheat, inferior to the yield, quality and
output of nutrients per unit of area. It is necessary
to maximize the potential of new varieties of
winter triticale, as the productivity of animals
largely depends on the varietal characteristics of
the culture. Triticale has attracted special attention
by its ability to exceed the parental forms on
productivity and quality, and resistance to adverse
soil and climatic conditions and the most
dangerous disease is not inferior to rye. The
generalized data of most researchers characterize
triticale as a factor in increasing the productivity of
animals and reducing the cost of feed per unit of
output, although in some cases there is a negative
trend in reduction of productivity by the
replacement of cereal grain components for winter
triticale. Due to the enrichment of compound feed
with triticale grain by enzyme preparations and
enzyme-probiotic supplements we can greatly
increase productivity, digestibility of main
nutrients, improve meat quality and reduce the cost
of feed per unit of output. We can increase the
productive effect of triticale grain by extrusion. In
physiological metabolic experiments, it is found
that by extruding of triticale grain the digestibility
of the main nutrients is increased by 1.3-1.8%.
Extruding of triticale before feeding promotes
slaughter yield and increases the levels of protein
in the muscle. The use of triticale in rations instead
of wheat reduces the cost of the diet, the cost of the
manufactured products and increases profitability.
The study of achievements of scientists from
various scientific organizations necessitates the
further breeding work with the culture of triticale
and requires the development of innovative
technological methods that can help reduce the
concentration of pentosans and beta-glucans in the
grain
The article presents materials on comparative study of the nutritional value of chickpeas and peas which are widespread in the arid zone of the Southern Urals. The scheme of the scientific and economic experiments involved the assessment of yield formation of chickpeas and peas during the vegetation. The study showed that different varieties of chickpea and pea have considerable difference in the content of nutrients. The amount of crude protein in the pea grain ranged from 25.1 to 26.8%, and chickpea – 21.7 - 22.9%. With regard to the fat, its highest concentration was in chickpea grain - 3.63%, on average, which is 1.16% higher than on average in the pea grain. The chemical composition of the pea and chickpea grain of different varieties points to differences in their composition and homogeneity within the culture. Basing on the study, we recommend to sow chickpea and pea grain in the middle of May to obtain the highest yield, this, in turn, has a positive effect on the nutritional value of the crops. When selecting plants for cultivation in the conditions of the Southern Urals, it is preferable to choose the variety of Madonna pea, which has a higher potential for yield than the chickpea variety Krasnokutsky-123. It has been experimentally found that chickpea grain has better moisture-retaining power in a bound condition during the vegetation period as compared to the relevant characteristics of pea grain, which is a positive drought-resistant value of crop. In structural elements of the harvest the peas showed the trend of the highest rates in comparison with the corresponding elements in chickpeas
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The author has studied the efficiency of diets with
kiselgura. Introduction of the kiselgura into the mixed
fodder for chicken-broilers favors increasing of live
weight by 4.8 % and average daily weight gain by 5.0%
The efficiency of replacement of sunflower oilcake by rapeseed oilcake from double-zero rapeseed varieties was proved for mixed fodders for pigs
Zoo engineering expediency of triticale utilization as part of combined fodder for pigs in their growing and finishing is studied. Triticale grain may be used as part of combined fodder for young pigs up to 50 %
The article gives theoretical and practical justification of sorghum use in animal feeding. The results of the study of organization of forage resources for livestock and poultry with the use of forage sorghum are shown. The ways and directions for the increase of nutritive value and productive effect in the diets for animals and poultry are determined
Application of enzymatic agent MEK CX-3 (as a component of combined feeds for broiler chicks) promotes live weight gain, feed conversion efficiency and safety of young birds