Name
Коnоnеnко Sergey Ivanovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
North-Caucasus Research Institute of Animal
Web site url
—
Articles count: 32
The author has studied the efficiency of diets with NATUPHOS 5000 enzyme preparation. Adding the enzyme preparation into the mixed fodder for chicken broilers favors increase of live weight by 4,5 % and average daily weight gain by 6,1 %
The author has studied the efficiency of diets with
kiselgura. Introduction of the kiselgura into the mixed
fodder for chicken-broilers favors increasing of live
weight by 4.8 % and average daily weight gain by 5.0%
In our country, great influence is given to the
production and consumption of agricultural animals'
meat with high consumer qualities. In this regard, the
livestock producers, particularly pigs, are to increase
production and improve product quality. Works on
using some natural mineral complexes - bentonite and
zeolites as a source of macro - and trace elements are
performed. The aim of the research is to study the
effects of bentonite feeding with free access on the
meat productivity and hygienic meat quality of young
pigs for fattening. Best effect on the slaughter indexes
of fattening gilts had bentonite feeding with free
access that resulted in significant superiority of the
animals in the experimental group over the control
counterparts on pre-slaughter live weight, slaughter
weight, chilled carcass weight, slaughter yield, carcass
length, the "muscle" eye area and the backgammon
weight. More favorable effect in the longest muscle on
the dry matter and protein synthesis was provided by
bentonite feeding with free access. Due to this, the
gilts of the experimental group were in significant
advance of their control counterparts in concentration
of dry matter and protein in meat. Along with this, the
longest muscle of young pigs on fattening in the
experimental group had the highest protein value, as
according to the protein-qualitative indicator the
animals of this group were in significant advance of
the control counterparts. Feeding bentonite clay with
free access had a strong detoxifying effect that is
against control counterparts of gilts in the experimental
group showed the significant reduce of lead, zinc and
cadmium concentrations in the meat
In the conditions of the North Caucasus during aflatoxin B1 tolerant level in the ration it is desirable to breed the broiler chickens cross “Smena-4” and “Ross 308” adding toxi-nyl preparation in the amount of 2 kg/t
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The aim of the research was to study the impact of
starter complete feed (CF) with different levels of
crude fat on the chemical composition of muscle tissue
and liver of young geese. During days 5-28 the poultry
was fed CF with 5.1-8.1 % crude fat, from 29- to 60-
day age – with 5.4 %. By increasing the level of crude
fat in starter CF there was a trend to an increase of the
phosphorus content in muscle samples of geese hip
and thigh - by 4.4-9.6 %, in the liver - by 27.2-53.5 %
(P≤0,05). The chest muscles of the young geese fed
high-calorie CF had 2.8-6.0 % higher fat content, than
that of counterparts in the first group. At the same
time, there was a tendency to a decrease in the
concentration of fat in the muscles of the hip and thigh
- by 1.9-5.0 % (P˃0,05). Increased level of crude fat in
CF did not have a negative impact on the accumulation
of heavy metals in the muscle and liver of geese, the
concentration of the studied elements was within the
permissible level established for food products
The article presents materials on comparative study of the nutritional value of chickpeas and peas which are widespread in the arid zone of the Southern Urals. The scheme of the scientific and economic experiments involved the assessment of yield formation of chickpeas and peas during the vegetation. The study showed that different varieties of chickpea and pea have considerable difference in the content of nutrients. The amount of crude protein in the pea grain ranged from 25.1 to 26.8%, and chickpea – 21.7 - 22.9%. With regard to the fat, its highest concentration was in chickpea grain - 3.63%, on average, which is 1.16% higher than on average in the pea grain. The chemical composition of the pea and chickpea grain of different varieties points to differences in their composition and homogeneity within the culture. Basing on the study, we recommend to sow chickpea and pea grain in the middle of May to obtain the highest yield, this, in turn, has a positive effect on the nutritional value of the crops. When selecting plants for cultivation in the conditions of the Southern Urals, it is preferable to choose the variety of Madonna pea, which has a higher potential for yield than the chickpea variety Krasnokutsky-123. It has been experimentally found that chickpea grain has better moisture-retaining power in a bound condition during the vegetation period as compared to the relevant characteristics of pea grain, which is a positive drought-resistant value of crop. In structural elements of the harvest the peas showed the trend of the highest rates in comparison with the corresponding elements in chickpeas
Studied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
The article presents information about the effect of enzymatic agent Ronozym WX on the efficiency, metabolic processes in the body of pigs and on hematological rate in compound feeds with triticale grain