Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Коnоnеnко Sergey Ivanovich

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
    North-Caucasus Research Institute of Animal
   

Web site url

Email

Kononenko@nm.ru


Articles count: 32

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218 kb

EFFECT OF FERMENTS ON PRODUCTIVITY

abstract 0871303006 issue 87 pp. 85 – 112 30.03.2013 ru 1958
The author has studied the efficiency of diets with NATUPHOS 5000 enzyme preparation. Adding the enzyme preparation into the mixed fodder for chicken broilers favors increase of live weight by 4,5 % and average daily weight gain by 6,1 %
240 kb

EFFECT OF FAT ADDITIVE ON THE PRODUCTIVITY

abstract 0931309009 issue 93 pp. 124 – 155 30.11.2013 ru 1439
The author has studied the efficiency of diets with kiselgura. Introduction of the kiselgura into the mixed fodder for chicken-broilers favors increasing of live weight by 4.8 % and average daily weight gain by 5.0%
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EFFECT OF BENTONITE ON MEAT PRODUCTIVITY AND QUALITY OF PORK GILTS

abstract 1181604044 issue 118 pp. 773 – 782 29.04.2016 ru 730
In our country, great influence is given to the production and consumption of agricultural animals' meat with high consumer qualities. In this regard, the livestock producers, particularly pigs, are to increase production and improve product quality. Works on using some natural mineral complexes - bentonite and zeolites as a source of macro - and trace elements are performed. The aim of the research is to study the effects of bentonite feeding with free access on the meat productivity and hygienic meat quality of young pigs for fattening. Best effect on the slaughter indexes of fattening gilts had bentonite feeding with free access that resulted in significant superiority of the animals in the experimental group over the control counterparts on pre-slaughter live weight, slaughter weight, chilled carcass weight, slaughter yield, carcass length, the "muscle" eye area and the backgammon weight. More favorable effect in the longest muscle on the dry matter and protein synthesis was provided by bentonite feeding with free access. Due to this, the gilts of the experimental group were in significant advance of their control counterparts in concentration of dry matter and protein in meat. Along with this, the longest muscle of young pigs on fattening in the experimental group had the highest protein value, as according to the protein-qualitative indicator the animals of this group were in significant advance of the control counterparts. Feeding bentonite clay with free access had a strong detoxifying effect that is against control counterparts of gilts in the experimental group showed the significant reduce of lead, zinc and cadmium concentrations in the meat
136 kb

DIGESTION EXCHANGE PECULIARITIES OF BROILER CHICKENS DURING FEEDING ECOLOGY DISTURBANCE

abstract 0871303033 issue 87 pp. 418 – 427 30.03.2013 ru 1823
In the conditions of the North Caucasus during aflatoxin B1 tolerant level in the ration it is desirable to breed the broiler chickens cross “Smena-4” and “Ross 308” adding toxi-nyl preparation in the amount of 2 kg/t
263 kb

DESIGN OF THERAPEUTIC PHAGE COCKTAILS BASED ON T4-TYPE BACTERIOPHAGES: ADVANTAGES AND DISADVANTAGES

abstract 1331709063 issue 133 pp. 823 – 850 30.11.2017 ru 804
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
1215 kb

CONSTRUCTION OF MUTANTS OF BACTERIOPHAGE T4 WITH REDUCED ANTIGENICITY

abstract 1341710034 issue 134 pp. 404 – 426 29.12.2017 ru 1422
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
145 kb

CONCENTRATION OF NUTRIENTS AND MACRONUTRIENTS IN MUSCLE AND LIVER OF YOUNG GEESE FED COMPLETE FEED WITH DIFFERENT LEVELS OF CRUDE FAT

abstract 1201606062 issue 120 pp. 914 – 923 30.06.2016 ru 564
The aim of the research was to study the impact of starter complete feed (CF) with different levels of crude fat on the chemical composition of muscle tissue and liver of young geese. During days 5-28 the poultry was fed CF with 5.1-8.1 % crude fat, from 29- to 60- day age – with 5.4 %. By increasing the level of crude fat in starter CF there was a trend to an increase of the phosphorus content in muscle samples of geese hip and thigh - by 4.4-9.6 %, in the liver - by 27.2-53.5 % (P≤0,05). The chest muscles of the young geese fed high-calorie CF had 2.8-6.0 % higher fat content, than that of counterparts in the first group. At the same time, there was a tendency to a decrease in the concentration of fat in the muscles of the hip and thigh - by 1.9-5.0 % (P˃0,05). Increased level of crude fat in CF did not have a negative impact on the accumulation of heavy metals in the muscle and liver of geese, the concentration of the studied elements was within the permissible level established for food products
141 kb

COMPARATIVE EVALUATION OF THE NUTRITIVE VALUE OF DIFFERENT VARIETIES OF PEA AND CHICKPEA GRAIN IN DROUGHT CONDITIONS

abstract 1071503092 issue 107 pp. 1416 – 1425 31.03.2015 ru 1243
The article presents materials on comparative study of the nutritional value of chickpeas and peas which are widespread in the arid zone of the Southern Urals. The scheme of the scientific and economic experiments involved the assessment of yield formation of chickpeas and peas during the vegetation. The study showed that different varieties of chickpea and pea have considerable difference in the content of nutrients. The amount of crude protein in the pea grain ranged from 25.1 to 26.8%, and chickpea – 21.7 - 22.9%. With regard to the fat, its highest concentration was in chickpea grain - 3.63%, on average, which is 1.16% higher than on average in the pea grain. The chemical composition of the pea and chickpea grain of different varieties points to differences in their composition and homogeneity within the culture. Basing on the study, we recommend to sow chickpea and pea grain in the middle of May to obtain the highest yield, this, in turn, has a positive effect on the nutritional value of the crops. When selecting plants for cultivation in the conditions of the Southern Urals, it is preferable to choose the variety of Madonna pea, which has a higher potential for yield than the chickpea variety Krasnokutsky-123. It has been experimentally found that chickpea grain has better moisture-retaining power in a bound condition during the vegetation period as compared to the relevant characteristics of pea grain, which is a positive drought-resistant value of crop. In structural elements of the harvest the peas showed the trend of the highest rates in comparison with the corresponding elements in chickpeas
137 kb

COMBINED USING THE NON TRANSDUCING E. COLI BACTERIOPHAGES WITH PROBIOTIC AT POST-WEANING PERIOD OF PIGS

abstract 0781204061 issue 78 pp. 775 – 785 30.04.2012 ru 2372
Studied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
125 kb

BROAD SPECTRUM ENZYMATIC AGENT RONOZYM WX IN HOG FEEDING

abstract 0681104020 issue 68 pp. 232 – 242 28.04.2011 ru 2327
The article presents information about the effect of enzymatic agent Ronozym WX on the efficiency, metabolic processes in the body of pigs and on hematological rate in compound feeds with triticale grain
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