Name
Skoblikov Nikolay Eduardovich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
North-Caucasus Research Institute of Animal
Web site url
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Articles count: 7
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
This work considers the needs and methods of phage
therapy of two bacterial infections in poultry. The
review examines the use of bacteriophages, from the
moment of their discovery to the present day. We
discuss the relevance of such diseases as
compilobacteriosis and colibacteriosis transmitted
from birds to humans, we show how to use
bacteriophages and their efficacy in models of adult
birds and chickens, including information provided on
the pilot study of phage therapy at a poultry farm. The
reported study was partially supported by RFBR,
research projects No. 16-44-230855 and No. 13-04-
00991 and № 16-44-230855-р_а
The isolation of E.coli phages from samples of natural
and waste water obtained during expeditions in the
different regions of Russian Federation was carried
out. The obtained phages (286 isolates) were tested for
their ability to lyse the pathogenic strains of E. coli –
pathogenic agents of pig colibacteriosis in Krasnodar
region. The study was conducted of their ability to
phage transduction, the molecular-genetic
characterization and biotechnological parameters of
selected bacteriophages. For first experimental design
of veterinary drugs was selected 5 coliphages having no ability of plasmids transduction. It has been shown
that all the investigated phages are representatives of
T4-type phages of family Myoviridae. The reported
study was partially supported by RFBR, research
projects No. 08-04-99111, 09-04-10132, 16-44-
230855
Studied the investigation of the therapeutic effect of experimental mixture of four non-transducing E. coli bacteriophages and it combinations with probiotic, designed on the basis of the endemic Lactobacillus paracasei strain, previously isolated from pigs in this age of the local population in the state, the survival rate and laboratory parameters of pigs with post-weaning diarrhea. The most influence on the correction of E. coli dynamics has a combined oral administration of phage medicine and probiotic preparation established. The most influence on the dynamics of titer phages has not only the combined using (both oral and intradermal) of phage medicine with the probiotic preparation, but also an isolated ingestion of phage mixture
The influence of four experimental phage preparations, designed based on non-transducing E. coli bacteriophages on pigs with post-weaning diarrhea studied. The influence of these phages in depending by methods of its applying (per os and intra cutis) on coli-titer and coli-phages titer studied. It is established, that the best survival rate and the clinical condition showed animals treated with the E. coli phage T4-type with lab. number «8» (till 5,52 lg CFU/g at 37-days age) in parallel with an increase in titer of phages (till 5,73 lg CFU/g at 40-days age). Intradermal (intra cutis) method of applying of experimental phage preparations (constructed on basis of phage “6” and phage “8”) showed the some positive effects on correction of negative dynamics of coli-phages titer in control group