Scientific Journal of KubSAU

The article presents the results of a study of the apple tree rosette disease found in a collection garden and having a viral nature. The rosetting has typical signs of manifestation. Sick plants develop narrow small chlorotic leaves. Rosettes of leaves on the upper cervical and lateral shoots are formed as a result of the convergence of internodes. The spread of the rosetting in the planting reaches 10-11 %. The prevalence of viral disease varies among the 44 varieties of apple trees of different ecological and geographical origin. So, the lesion of rosetting was noted on single trees in the varieties of Gala Redi Red, Fujiko, Red Free, etc., and it was a complete defeat in the variety of Piros. A different degree of the manifestation of the disease on the trees was established: from partial manifestation on individual shoots, to aggressive damage to the entire crown of the tree. On young trees bearing fruiting (3-4 years from planting), the rosetting reduces the yield of apple trees by 36 %, ¼ part of the fruits on the trees are small in size compared to the fruits of healthy trees. The infectious nature of the rosetting is confirmed by the transfer of the disease on the most affected Piros variety using summer budding

The article presents results of the assessment of the efficiency of non-hormonal preparations which were not earlier applied in culture in vitro with high physiological activity (the preparations received by production of furfural, and also derivatives and compositions of organic acids) during regenerations of microshoots of plum, comparison of their influence with influence of growth regulators which are traditionally used in clonal micropropagation. These experimental preparations were received when processing waste of agricultural production. In this work we used: technology of clonal micropropagation of plants of in vitro, statistical data processing by method of the dispersive analysis. The objects of researches were microshoots of plum of a Stanley variety. We have established that on mediums with the preparations "Universal", sodium succinate, potassium succinate, amber acid, L-1 the large, intensively colored plum microshoots develop surpassing control (medium with BAP of 1 mg/l, IBA of 0,1 mg/l, gibberellic acid of 0,5 mg/l) in morphometric parameters. Thus, the preparations "Universal", sodium succinate, potassium succinate, amber acid, L-1 in concentration of 4,0 mg/l proved as the growth factors which are favorably influencing on plantlets’ regeneration and a qualitative condition of microshoots of plum

The stage of introduction of plantlets to in vitro culture is an important stage of technology of clonal micropropagation of plants. For the purpose of decrease in a share of the plantlets that were lost from an infection and increase of their regeneration ability, sterilizers and antibiotics, effective and safe for apple rootstocks’ plantlets wee allocated, and also favorable terms of introduction to in vitro culture were allocated. As a result of the conducted researches, we have established that on influence on an nutrient medium and plantlets infection and on growth and development of plants, beneficial effect on apple rootstocks’ plantlets has an antibiotic nystatin of 200 mg/l: the reproduction coefficient on the medium with this antibiotic is equal 4,3, in the same concentration nystatin has the sanifying effect of 60-75% for stocks of SK 2 and MM 106. During researches of sterilizers for apple rootstocks’ plantlets SK 2, SK 3, SK 4, SK 7, MM 106 as an alternative to widely applied highly toxic sterilizer corrosive sublimate (the first class of danger) were picked up effective and safe preparations for sanitation of plantlets from an infection, such as the household preparation "Whiteness" (sodium hypochlorite) in cultivation 1:2, low-dangerous substance of the fourth class of danger (a share of viable plantlets of 75,5% from initially introduced), and also fosfopag, a preparation of the fourth class of danger (a share of viable plantlets of 65% from initially introduced). The favorable term for a meristem of apple rootstocks’ plantlets’ introduction to in vitro culture are phases of buds’ burgeoning (March) and the intensive growth of shoots (May – June)

Clonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way

The analysis of fruit crops saplings production in the Krasnodar region is presented. The problems of the modern fruit crops saplings production in the region are allocated and possible ways of decision these problems are given

The studies’ results of the Agrospan protection mate-rial influence on the strawberries mater plants productivity are listed

The results of the study of physiological and biochemical parameters of plum plants, changing when infecting plants Plum pox potyvirus (PPV) are shown. It was found that the infected leaves at the stage of full development of the content chlorophyll a reduced by 30 % in variety Stanley and by 6 % in Kabardinskaya rannaya, chlorophyll b in less than 49 % at Stanley, 37% in Kabardinskaya rannaya, carotenoids are less than 22 % in the variety Stanley and by 11% in the variety Kabardinskaya rannaya . Leaves infected with the virus pox (PPV), in comparison with not infected, the amount of protein were decreasing: in the variety Stanley by 21 %, in the variety Kabardinskaya rannaya by 28 %. In the period of active growth of the leaves change in the content of pigments and protein is more intense. The content of lignin in the tissues of leaves, infected with the virus pox (PPV), in comparison with not infected in the variety Stanley was below on 13 % and in the variety Kabardinskaya rannaya - 8 %. The total content of water of infected tissue on the stage of a full-time development is reduced in patients leaves as compared with not infected 11 % in the variety Stanley and by 1 % in the variety of Kabardinskaya rannaya. Throughout the period of development of foliage studied parameters vary depending on the variety