Scientific Journal of KubSAU

The article demonstrates the results of therapeutic and preventive effectiveness of the use of lactic acid bacteria – Lactobacillus brevis and Lactobacillus parabuchneri in laboratory animals, in particular mice and rats. They were isolated from the gastrointestinal tract of a wild quail in experimental violation of the intestinal microbial background. Artificial imbalance of the intestinal microbiota of experimental animals was caused by the use of the antibiotic. As a result of tests it was established that the application of probiotic cultures improved microbial disorders of the gut, however, the use of their consortium in the probiotic promoted consistent clinical result characterized by an elevated level of representatives of the normal flora (lacto- and bifidobacteria) and reduction of the number of representatives of pathogenic and conditionally pathogenic microbiota, with subsequent achievement of a level characteristic of healthy animals

The article presents a comparative assessment of the meat productivity of young cattle of the two most common intensive meat breeds in the Russian Federation (Aberdeen Angus and Hereford) in comparison with the most numerous native in the South of the country domestic breed, which is Kalmyk. Intensive rearing from 9 to 18 months of age was carried out in the conditions of industrial fattening complex on rations not containing juicy forages. In the process of 273-day intensive rearing, in bulls of the tested breeds average daily live weight gains from 1455 to 1526 grams were achieved. During this period, an absolute increase was obtained in Aberdeen-Angus bulls-416.6 kg, in peers of Hereford and Kalmyk breeds-413.6 and 397.2 kg. The highest pre-slaughter live weight and slaughter rates were noted in Aberdeen-Angus bulls, having a yield of a pair carcass of 59.4 % and a slaughter yield of 62.8 %. The meat of Aberdeen-Angus bulls has the highest content of fat and dry matter, but they have less protein, lower pH and moisture binding capacity and a higher loss of meat juice during cooking, compared with the bulls of the Kalmyk breed. It was confirmed that the coefficients of transformation of protein and energy into products are closely related to the intensity of growth and weight of the carcass. The most effective of these indicators were in bulls of Aberdeen-Angus, then Hereford and Kalmyk breeds. A similar pattern between the breeds was manifested in terms of profitability and cost recovery

This article provides an overview of the need of the use of probiotic supplements and medications in the diets of insects, in particular bees and their families. We present a list of both Russian and foreign probiotics and their complexes, which are used in beekeeping for the treatment and prevention of various diseases, are shown. Their effectiveness is shown not only for the prevention and treatment of microbial disorders in the gastrointestinal tract of bees due to the manifestation of antagonistic properties in relation to opportunistic and pathogenic microflora, but also the possibility of their use as tools that can increase the immune status, the safety of bee families, and life expectancy. The article shows data on the positive effect of probiotics on the physiological and biochemical parameters of honeybees, activation of metabolic processes in the formation of a fat body, growth of strength and weight of larvae, development of pharyngeal glands, etc.

The article provides a convincing argument for the preference of wool and fur products over artificial and synthetic textile fibers. It is noted that artificial fibers and synthetics have negative ionization, while natural wool has positive ionization. It is emphasized that negative ionization is far from harmless to humans. The analysis of the state of sheep breeding in the Russian Federation is given and this industry in the Rostov region is characterized against this background. The data on the number and productivity of sheep of the main age and sex groups, the production of young animals per one hundred ewes are presented. The characteristic of tribal resources of the country and region is given. The breeding base of the industry in the Russian Federation is represented by 205 breeding organizations, including 3 breeding and genetic centers, 44 breeding plants, 145 breeding reproducers and 13 gene pool farms, which contain 1389.8 thousand sheep (39.0 % of their total number in agricultural organizations and 6.6 % of the sheep population in farms of all categories), including 904.7 thousand Queens (35.0 and 6.2%, respectively). The breeding base of the Rostov region has eleven farms, of which 6 breeding plants for such breeds as the Soviet Merino and salskaya and 5 breeding reproducers. In addition to these breed resources, there are breeding reproducers for the qigai and edilbaev breeds in the region. Total number of breeding sheep in the region is almost 44 thousand. The article indicates main problems in the development of the industry and ways and means to solve them

Improvement of domestic breeds of Merino sheep remains an important task. The reorientation of the industry to wool-meat and meat-wool direction of productivity can contribute to the restoration of domestic sheep breeding. An example of such conversion is a relatively young breed called Dzhalginsky Merino, created in the Stavropol region on the basis of the Stavropol breed with the involvement of resources of domestic and world breeding. In the current situation, it seems relevant to use Dzhalginsky Merino, in order to improve the meat and wool qualities of sheep of domestic fine-wool breeds. Obtaining a new genetic combination of animals, formed by combining the genotypes of breeds Salsky, Stavropol and Dzhalginsky Merino will increase the efficiency of the industry in a market economy and will provide a more complete use of the productive potential of the breed. The results of studies on obtaining and use of animals, new genetic combinations of a wool – meat direction of productivity, with high energy of growth, meat and wool productivity, improves the efficiency of Merino sheep are an important theoretical justification for making a contribution to zootechnical science, with the aim of improving fine-wool breeds of sheep and can be used in practical work in the production of high quality lamb

The article reflects information characterizing the significance of mastitis and endometritis in the indicator of premature disposal of cows from herds of large farms in the western regions of the Krasnodar region: an average of 30% of the total culling of cows. The effectiveness of veterinary measures in Pravoberezhny JSC of the Temryuk district for mastitis and endometritis in cows was assessed: 81-95%; fertilization index value ‒ 1.8; the duration of the service period exceeds the requirements by 20 days. The studies the nature of the influence of the drugs called Sepranol and Uteroton on the calving process and the occurrence of postpartum complications in cows from the herd, which was formed with violation of breeding principles. The use of the drugs Uteroton and Sepranol contributed to a 50% decrease in the calving index in calving cows by 50% compared with the results of the combined use of estrophan and oxytocin. The use of Uteroton reduced the damage from the lack of milk by 87880 rubles, and the use of Sepranol - by 171164 rubles. We have given practical recommendation on the use of Uteroton and Sepranol for veterinary effects on the body of cows in the early postpartum period in order to reduce the occurrence of complications

In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract

Pasteurella multocida is an important respiratory pathogen of cattle. OmpH Protein is a major protective antigen of bacteria has been well studied in avian strains. In the literature there are no data available for the study of a variety of sequence of this protein among isolates with cattle respiratory pathology. There have been described several genes associated with the virulence of the bacterium in respiratory disease of cattle, but none of the authors compared the frequency of detection of these genes with the pathogenicity for laboratory animals. The aim of our study was the development of allele-specific PCR to determine Omph-types of Pasteurella multocida and the search for new approaches to assess the pathogenicity of isolates of bacteria. Total amount of 83 isolates allocated from the lungs of calves with respiratory pathology was investigated. All isolates belonged to groups A or D (isolates 63 and 20, respectively). Among isolates of capsular serogroup A we revealed 6 types, most propagation types were A1 and A2. All isolates of capsular serogroup D were one omph- type. In 16 out of 23 farms there were identified isolates of only one omph-type, 4 - 2 types, 3 - three types. The frequency of gene hgbb - hemoglobin binding protein correlated with pathogenicity of isolates for white mice. The developed allele-specific PCR along with hgbb gene detection can be used for screening and studying the properties of antigen and circulating pathogenic isolates and selecting a candidate vaccine strains

We have studied biochemical changes in the body of cattle, sick with nodular dermatitis in the initial stage of the clinical manifestation of the disease. It is proved that in case of disease in animals there is a significant increase in the level of creatinine up to 295.5 mg%. The excess of the norm by 82.1%. In treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the norm (162.4 mg%). In the blood serum of patients, the carotene content was reduced to 0.09 mg%, which was restored to normal three days after the start of treatment (1.0 mg%). The glucose level at the beginning of the disease was 18% below normal and reached the lower limit of the norm 8 hours after the start of treatment. When studying the activity of CKK, it was found that the activity of the enzyme in animals before the introduction of a 5% solution of sodium hydrogencarbonate is 82% higher than the upper limit of the norm. After the treatment, this indicator returned to normal. The inorganic phosphorus index did not reach the physiological norm (2.5 mmol / l) before and after treatment, and remained 11.6% higher (2.9 mmol / l), which indicates a violation of mineral metabolism in sick animals. The level of reserve alkalinity began to recover after 8 hours of observation and reached a minimum limit of 48.91 vol. % CO2. During the entire period of the experimental studies, before and after the administration of the drug, there were no significant changes in the biochemical parameters of total protein, calcium, vitamin E and urea. The nature of the established changes varied within the limits of physiological norms. The use of a 5% solution of sodium hydrogen carbonate in nodular dermatitis increases the effectiveness of symptomatic treatment, reduces the likelihood of complications and stimulates the production of specific antibodies against viruses and bacteria. We have recommended intravenous injection of 5% sodium hydrogencarbonate solution to sick animals at the rate of 1 ml per 1 kg of live weight of the animal three times with an interval of 3 days

A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855