Scientific Journal of KubSAU

The article is concerned with the use of functional feed additives from pumpkin fruits and alfalfa juice for the poultry industry. In the study of laying hen it has been found that the use of a feed additive in-creased pumpkin paste content in serum and egg yolk carotenoids is more than two times, and the concentration of vitamin A in these tissues increased slightly, not exceeding 20%. Livability and productivity of poultry increased and average expendable fodder per head per day decreased. Economic calculation showed that the use of pumpkin paste reduces the cost of production of eggs. The use of alfalfa juice coagulates in diets for broiler chickens resulted in an increase in the concentration of carotene in the liver by 22,7-46,8% relative to control. The juice addition also increased the content of vitamin A in the liver by 27.9%, increase the safety and efficiency of the bird. The authors draw certain conclusions from experiment results that the use of functional additives in poultry from the fruit of pumpkin and alfalfa juice is an alternative to synthetic vitamin preparations and produce a more environmentally friendly products

This review contains the information about the role of annelids in maintaining soil fertility. Recommendations based on authors’ experience and scientific literature are given for vermicultivation, biomass use of Californian worms in animal husbandry and vermicompost use in crop production. Furthermore, detailed description of the methods of cultivation of worms is given

A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855

The article provides an overview of materials of conferences on problems and prospects of the industry of cattle breeding

The article covers the study of the influence of sodium hypochlorite different concentrations on the quail organism in various schemes of its application

The optimum conditions of cultivation of probiotic association bacteria (structures of mother waters and industrial nutritious environments, optimum of temperature and time of cultivation) are determined; therefore it was allowed to develop the “know-how” of the Bacell fodder additive. On the basis of the carried out experiments of the application of Bacell on birds it is possible to make a conclusion about expediency of its application at cultivation of the hens as the fodder additive. Its use allows to increase efficiency, safety layers, and to increase the competitiveness of manufacture of eggs and meat of the hens in general

In the present article the authors provide an overview of the results of experimental works for the use of electro activated water solutions in agroindustrial complex

In the work, we have presented the information of elaboration of a manufacturing process of Mycocel feed additive with the cellulolytic activity for poul-try industry. Manufacturing process includes follow-ing steps: stock culture maintenance and storage of Trichoderma lignorum 81-17, growing fluid culture of microscopic fungus in sucrose yeast extract me-dium, feed additive with cellulolytic properties out-put and quality control, packaging, storage and disposal of waste. We have shown that the Mycocel is non-toxic feed additive for protozoa and warm-blooded animals (laboratory mice and quails). This study demonstrated total population livability in the experimental group with feed additive. Quail body-weight of experimental group was higher by 6% as compared to the control and feed consumption per 1 kg of live weight of bird was 3,58 kg, 7,5% lower than the control

The present review highlights the applications of electro-activated aqueous solutions in biotechnology and the food industry

The article is an analysis of the results of three years of work with sexing sperm in one of the two farms of the Kuban region which have implemented this tech-nology