Scientific Journal of KubSAU

The article presents a comparative assessment and peculiarities of nuclear magnetic relaxation characteristics of rapeseed and sunflower lecithin. It was established, that lecithin’s nuclear magnetic relaxation characteristics, namely, protons’ spin-spin relaxation time and amplitudes of nuclear magnetic relaxation signals of lecithin components, depend on content of oil’s fat acids and phospholipids, contained in the lecithin. Comparative assessment of protons’ spin-spin relaxation time of rapeseed and sunflower lecithin components showed, that for their identification protons’ spin-spin relaxation time of the lecithin’ first component at 40°C and 60°C temperature, when differences in values of protons’ spin-spin relaxation time of the sunflower and rapeseed lecithin’ first component are most obvious, should be used as an analytical parameter. Comparative assessment of amplitudes of proton’s NMR signals of sunflower and rapeseed lecithin components showed, that relations, established earlier for sunflower lecithin between mass share of phospholipids (substances insoluble in acetone) and the sum of amplitudes of proton’s NMR signals of the third and fourth components, cannot be applied to rapeseed lecithin; that is for the development of an express method for determination of mass share of substances insoluble in acetone (phospholipids) in rapeseed lecithin, it is necessary to carry out additional research with the purpose of clarifying the mentioned relations

In recent decades, Russia has increased the demand for high oleic sunflower oil and, consequently, for the raw material from which it is derived – high oleic sunflower seeds. Currently existing methods of control of mass fraction of oleic acid in the seed oil of sunflower, have significant drawbacks, due to which sharply raises the question of the development of the Express methods of control of mass fraction of oleic acid. The study presents the results of determining the mass fraction of oleic acid in the oil of sunflower seeds, obtained by known methods (GC and ri), and developed a method based on NMR. The developed method of determining the mass fraction of oleic acid in sunflower seed oil has a number of advantages compared with the known: is not destructive (does not require the extraction of oil from seeds), does not require special sample preparation excludes the use of toxic chemicals, the analysis duration is 5 minutes, ensuring high representativeness of the sample, and the simplicity and automation of the analysis process reduces the requirements for qualifications of laboratory personnel. The developed method of determining the mass fraction of oleic acid in the oil of sunflower seeds can be carried out simultaneously with the determination of oil and moisture content of seeds on commercially available analyzers, but I hope it 1006М, which is currently applied at more than 250 companies of oil and fat industry

The article presents data, on the basis of which we have concluded that the oil and phospholipids contained in soy lecithins, the degree of unsaturation of fatty acids differ significantly from oil and phospholipids contained in sunflower lecithins, with differences in the degree of unsaturation of fatty acids of oil and phospholipids contained in sunflower and soy lecithins, leads to differences in the content of resonating protons, and, consequently, the level of amplitude values of NMR signals of protons of the investigated lecithins. It is established that the values of the amplitudes of the NMR signals of protons of each of the four components of sunflower lecithins differ from the values of the amplitudes of the NMR signals of protons the component of soy lecithins, which confirms the differences in the composition and content of fatty acids lecithins studied. Considering quantitative characteristic of mass fraction of substances insoluble in acetone, i.e. phospholipids, is the sum of the amplitudes of the NMR signals of protons of the third (A3) and fourth (A4) component, was built the graphical dependence of the mass fraction of substances insoluble in acetone, from the value of (A3+A4)/ASIS, % which is described by the equation (correlation coefficient R2 =0,9911) of the following form: y=0,8177x +2.52. Based on these data, we have developed a rapid method of determining the mass fraction of substances insoluble in acetone, soy lecithin, eliminating the use of toxic organic solvents, is environmentally safe, which is protected by RF patent for the invention

The article provides an overview of Express-methods of quality evaluation and identification of lipidcontaining plant raw materials (oil seeds) and its processed products (oil cakes, oilseed meals, vegetable oils, lecithins) using pulse method of nuclear magnetic resonance. Development of express methods of quality evaluation and identification of lipid-containing plant raw materials and products of its processing is a priority, as these methods are environmentally safe and also allow us to regulate technological conditions of refining processes, are characterized by low error of determination, i.e., high precision, and, in some cases, it is only possible to use because of its non-destructive effects on the analyzed sample. Currently, we can distinguish the following main directions of quality evaluation and identification of lipid-containing plant raw materials and products of its processing on the basis of the pulse method of nuclear magnetic resonance: assessment of quality and identification of oilseeds (sunflower, canola, flax, soybeans); evaluation of quality of products of processing of oil raw materials (cake and meal); the quality evaluation and identification of vegetable oils; assessment of the quality of the lecithins obtained from vegetable oils. The methods of quality evaluation and identification of lipid-containing plant raw materials and its products are patented and have several advantages: short time (5 minutes) spent on the implementation of the single analysis; eliminates the use of toxic chemicals; does not require special training of laboratory personnel; non-destructive character, which is especially important for oilseeds from the point of view of breeding work. On the basis of the conducted survey we can conclude that the pulse method of nuclear magnetic resonance has found wide application for assessment of quality and identification of lipidcontaining plant raw materials and products of its processing, through rapid capabilities, high accuracy and ease of analysis

To determine the mass fraction of linolenic acid in flax seed oil the authors developed a rapid method based on the pulse method of nuclear magnetic resonance with the use of NMR analyzers low resolution. This method has some significant advantages compared with the known method on the basis of chromatographic method for the determination of the mass fraction of linolenic acid in the oil extracted from the seeds of flax, namely, does not require additional sample preparation (extracting oil by solvent), eliminates the use of toxic chemicals, requires a much lower investment of time to conduct a single analysis, highly automated, and eliminates the influence of human factor on the research results. The method is based on the revealed correlation between the weighted average time spin-spin relaxation of protons contained in the triacylglycerols of the oil of the flax seed, and a mass fraction of linolenic acid in the seed oil at a temperature of 23ºc. In article results of research of influence of temperature on the weighted average time spin-spin relaxation of protons contained in the flax seed oil. Installed directly proportional linear relationship between the temperature of flax seeds and measured the average time spin-spin relaxation of protons oil contained in the flax seeds with the correlation coefficient is 0,930, the temperature change of the sample of seeds at 1 ° C leads to the change of the coefficient is 0.011 kt. The developed methodaccuracy in the temperature range from 20 to 26 ºC