Name
Gudkov Georgy Vladimirovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Medical University
Web site url
—
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Articles count: 3
Trophoblast cells circulating in maternal blood may
serve as potential sources of genetic information for
screening in obstetric practice approaches to
noninvasive prenatal diagnosis. The material for the
study was whole blood of pregnant women of
gestational ages of 8-12 weeks in a volume of 10 ml.
stabilized with heparin. Enrichment was performed by
the method of gradient centrifugation and magnetic
separation (CD45–) with fluorescent staining negative
fractions labeled with antibodies to antigens of
trophoblasts (HLA-G, and Trop-2) to conduct flow
cytometry and sorting of cells on glass slides. Single
cell candidates using laser micro dissection were
transferred into a vial for subsequent whole-genome
amplification, providing sufficient representativeness
of their genome. Fetal origin of the genetic material
was confirmed by comparison of alleles of the HLA
genes of the parents and cells candidates. It is possible
to improve significantly the accuracy and versatility of
non-invasive prenatal diagnosis using comparative
genomic hybridization using chips (аCGH)
The research shows the possibility of extended
preimplantation genetic screening (PGD) that allows
to improve the strategy of selection of embryos that
satisfies not only the requirement of the absence of
chromosomal abnormalities, but also includes their
additional check for predisposition to various diseases,
as well as the choice of the embryo with the most
optimal HLA haplotype in cases with expressed
compatibility of parents for HLA-genes
Isolation of fetal cells from the blood of a pregnant
woman is more relevant for receiving the information
of the genome of a fetus and implementation in clinical
practice, non-invasive screening methods for prenatal
diagnosis. The work proposes efficient ways of sorting
trophoblasts circulating in maternal blood for the
diagnosis of fetal aneuploidy in early gestation by
analyzing microsatellite marker of the chromosomes
that most prone to numerical aberrations (13, 18, 21, X
and Y). A set of microsatellite markers allowed to
reveal aneuploidy in three pregnant women, and also
to exclude mosaicism and contamination of samples
with maternal cells. A retrospective analysis of data on
invasive cytogenetic studies (chorionic villus
sampling) confirmed the results of the study