Scientific Journal of KubSAU

The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences