Scientific Journal of KubSAU

The article presents the data on the formation of the embryonic fruitfulness of central ovaries of wintering buds of the group of technical grape varieties with white berries - White Muscat; Pinot White, Chardonnay, Citron Magaracha, Pervenets Magaracha; Riesling, Viorica, Riton, Crystal in the conditions of Anapa-Taman zone. There were revealed the rates of embryonic fruitfulness of central ovaries of buds of studied cultivars and fruiting indices of vegetative shoots developed from them. In all studied grape varieties there was revealed a high percentage of fruitful buds from 84,1 in the variety of Riton at 97.2 at Viorica; and the percentage of fertile vegetative shoots from 81,8 at the variety Citron Magaracha to 97.2 in the variety White Muscat. At the leveled load of bushes, vegetative shoots and the same scheme of planting of bushes (3 x 2 m), the highest yield in terms per hectare showed the varieties Pervenets Magaracha, Viorica, Riton, Crystal, Riesling and Citron Magarach. When assessing the economic efficiency the highest net income and level of profitability were identified in the varieties of Citron Magaracha, Pervenets Magaracha, Viorica and Riton. In order to determine which buds will give us shoots with large, well-developed (well-differentiated) buds, and which will not give (weakly differentiated), it is necessary "to look inside a bud". But even already formed germs of inflorescences in the bud are able in a few days in spring or dedifferentiate or degrade depending on the influence of external conditions. Scientists have learned to use this ability to increase the maximum possible yield in years of severe damage of grape by frosts. Firstly, in frosty winters the central buds wither out. The replacing buds usually have poor fruiting and bad productivity during years. In such cases, it is more profitable to conduct a small cutting of angle buds arranged in a circle at the base of a shoot. At the beginning of the second vegetation phase, these buds will obtain the great bulk plastic substances, which will cause the dedifferentiation of rudiments of inflorescences in them. Thus, it is possible not only to restore rapidly the normal shape of a bush, but to obtain a good harvest this year

Methodological aspects of conduction of researches on study of regeneration activity of grape cuttings by students of secondary schools including by members of Small Agricultural Academy of Kuban are cited in the article

The article reviews the results of studying the influence of mineral fertilizers on the qualitative indices of the Merlot grape variety in the Anapo-Taman zone of the Krasnodar region. The technology of cultivation of grapes on the experimental plot was in accordance with the accepted for the given zone and culture. Agrobiological work was carried out at the optimal time and had a high quality of execution. Grape bushes are laid in a 3.0 x 2.0 m pattern. Formation - a twoshoulder horizontal cordon. On the bushes we formed the same load of shoots and bunches. Scheme of the experiment: option 1 – no fertilizers (control); option 2-superphosphate with a mixture of potassium salt (P90K90) from the autumn; option 3 –nitroammofoska (N120P120K120) from autumn; option 4 – ammonium nitrate (N60) in early spring. Analysis of the results of studies on the study of biological features of growth, fruiting, yield and quality of Merlot grape varieties on the background of the use of mineral fertilizers shows their high efficiency in the conditions of the AnapoTamanskaya zone of the Krasnodar region and can be recommended for use in production conditions. Mineral fertilizers ensures the receipt of dry bulk wine of superior quality control sample (without fertilizers) the concentration of tartaric acid 17.3 25.1 percent, phenolic compounds 17.1 – 40.0 %, and alcohol content of 7.2 and 9.3 %, titratable acids 11.8 and 13.7 %, given extract by 17.4 %, with a decrease of the mass concentration of total sulfur dioxide and pH. The most effective is the introduction of NPK (N120P120K120)

The research results on regeneration activity of wilding cuttings of phylloxeraresistant varieties of grapes influenced by heteroauxin treatment are analysed in the article. The preliminary studies were held in All-Russian research institute of viticulture and wine-making of Ya.I. Potapenko using cuttings of 3 grape varieties – Berlandieri x Riparia Cober 5bb, Riparia x Rupestris 101-14 and Berlandieri x Riparia CO-4. The cuttings were soaked during 48 hours in 0,005 % heteroauxin and were implanted in sand in adjustable conditions of temperature, humidity and aeration. In 2012-2013 experiments were continued in Kuban State Agrarian University. The varieties Gravesak, RSB and Berlandieri x Riparia Teleki 5C were added to the experiment. Three-items buds were shooted in damp steamed sawdust in a film hothouse. In preliminary researches application of heteroauxin has allowed to increase root-growing by 20,3-40,0 %. The greatest increase of root-growing (40 %) was observed on variety СО-4, that is characterized by the weakest potential rhizogenesis activity, and the least (20,3 %) on control variety Cober 5 bb. Next researches show that treatment of cuttings basal ends by 0,01 % heteroauxin activated their regeneration process, that was expressed in: increase in sprouts length – variety 5 C, 101-14 and Gravesak by 23,1- 157,6 %; increasing of cuttings root-growing from 16,7 % on variety 5C to 60,0 % on variety Cober 5 bb, the quantity of cuttings with not fewer of 3 roots from accordingly 13,0 % to 54,7 %; increasing of roots from 0,7 on variety СО-4 to 2,7 on variety 101-14 or in 1,7-2,7 times. The application of heteroauxin on variety RSB did not show positive results, because of the variety peculiarities

The article presents the results of studies on the effect of processing varieties of grape cuttings of Pervenets Magaracha using alternating electromagnetic field on their regenerative skills. Processing the cuttings with alternating electromagnetic field was carried out using an induction coil for 5,10,15 and 20 min. As a control option, the cuttings were soaked in water, as a standard option – the cuttings were soaked for 24 hours in a 0.01% solution of IAA. Rooting was performed in vessels with water at optimum temperature conditions. It was found, that the use of IAA for the first time led to the inhibition of blooming buds. EMF treatment of cuttings with each exposure has a stimulating effect on this indicator. Application of EMF exposures in the 5 to 15 minutes resulted in a significant increase in the length of shoots, compared to the control variant and the option to IAA. Processing cuttings with EMF exposures at 5,15 and 20 minutes resulted in a significant increase in rooting compared with the control. Maximum rooting was obtained at an exposure of 15 minutes. It was the same as in the version with a standard rooting stimulant - IAA. However, the highest yield of cuttings with 3 roots and ended up in a version with a 20-minute exposure. Maximum number was noted on the heel of the roots cuttings option with IAA. Treatment of cuttings with EMF exposure 5, 10 and 20 minutes, has also led to a significant increasing of this indicator, respectively, 45.5; 22.7 and 79.5%. The maximum increase in the number of roots was obtained in the variant with exposure 20 minutes. Thus, this option should be considered as the best one

The article reviews the results of the study of the influence of treatment of Saperavi grapes with the growth regulators (Biodux and Avibif) on the quality of grape must and wine in the Anapо-Taman zone of the Krasnodar region

The article presents the results of three-year researches of root-formating ability of grafts of 9 resistant table and technical grape varieties of native and foreign selection at their rooting in water. Among studied indexes of root-formaing ability of grafts, the best varieties were Moldova, Lyana, Riton and Pervenets of Magarach

Researches on non-root additional fertilizing of grapes with complex water-soluble fertilizer called Nutrivant plus have been conducted in corporation "Pobeda" in Temrjuksky area of Krasnodar territory on the implanted plantings of technical grade Rhine Riesling. In the test, the following kinds of Nutrivant plus were used: oleiferous (N0P20K33), sugar beet (N0P36K24), grapes (N0P40K25), universal (N19P19K19), grain (N6P23K35). Each mark of Nutrivant plus contained a certain set of microelements. The test consisted of six variants:1 variant - without additional fertilizing(control); 2 variant: grapes - before flowering, grapes - a growth phase of berries (a berry about a pea), oleiferous - the beginning of maturing of berries; 3 variant: universal - before flowering, universal - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 4 variant: grain - before flowering, grain - a growth phase of berries (a berry about a pea), oleiferous- the beginning of maturing of berries; 5 variant: Sugar beet - before flowering, a sugar beet - a growth phase of berries (a berry about a pea), a sugar beet - the beginning of maturing of berries; 6 variant: universal - before flowering, grain - a growth phase of berries (a berry about a pea), grapes - the beginning of maturing of berries. Norm of the expense of fertilizer is 3 kg/hectares. The expense of a working liquid - 700 l/hectares. The result was, that in the third, fourth and fifth variant of the test the increase in a crop from a bush happened accordingly on 7,5, 13,8 and 17,8 %. The maximum increase of a crop in the fifth variant was accompanied by increase in mass concentration of sugars in juice of berries on 0,6 g/100 см3и decrease titratable acidities for 0,6/dm3 As a result triple not root additional fertilizing of grapes plants grade Riesling with Nutrivant plus (universal - before flowering, grain - in a growth phase of berries and grape - in the beginning of maturing of berries) promoted more optimization of a water mode of leaves, increase in weight of a cluster, a crop from a bush and productivity, mass concentration of sugars and phenolic substances in juice of berries

The article presents the research results of eight grapes rhizogenesis of different genetic origin of the cuttings after treatment with 0.01% solution of IAA for 24 h. It was found, that the activity of regenerative processes in grape cuttings depends on the varietal characteristics, storage conditions and the activation of IAA. It leads to disruption of existing natural correlations, including suppressing the longitudinal polarity. Root-growing ability of cuttings is a genetically determined feature: the effect of IAA did not cause the stability of the positive reaction; it is determined by genotype and depends on the quality and physiological condition of the cuttings

Researches were carried out on three-eyed cuttings of stock of phylloxera-resistant grape varieties Berlandieri×RipariaCober 5BB (Cober 5BB), Riparia× Rupestris101-14 (101-14), Berlandieri × RipariaСО-4 (СО-4), Gravesak and RSB. Cuttings of presented varieties were wetted during 24 hours in water and after preliminary drying of the surface were covered in the upper part with antitranspirant, under the temperature about 90°С. Then 40 cuttings of every variety were placed with lower ends for 24 hours in 0,01% heteroauxin solution or for 8 hours in 1% Radix Plus solution. Cuttings of the control variant were placed in water. After treatment cuttings, were couching in a film greenhouse on a heated rack in humid steamed sawdust. In the result of the present researches there was determined that the wetting of basal ends of cuttings of stock phylloxera-resistant varieties in 1% Radix Plus water solution plus during 8 hours leads to the essential activity of regeneration processes in them. The expansion of the length of shoots on rooted cuttings of the experimental variant amounted for 19,2–154,5 %, the rooting of cuttings - 23,3–76,7 %, the output of cuttings having at least three roots – 33,0–78,1 %, the number of calcaneal roots– 80,8–257,1 %. The maximum increase of the length of shoots under the influence of Radix Plus was observed at varieties of Gravesak and СО-4, the rooting – Cober 5BB and CO-4, the amount of cuttings at least three roots and average amount of roots on them –101-14 and Cober 5BB. The Radix Plus caused the great stimulating influence the rootforming ability of cuttings of stock varieties than heteroauxin, the standard stimulator of root-forming