Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Troshin Leonid Petrovich

Scholastic degree


Academic rank

professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

Web site url

Email

lptroshin@mail.ru


Articles count: 169

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BIOTECHNOLOGY – SCIENCE AND SECTOR OF AGRICULTURE

abstract 1161602106 issue 116 pp. 1695 – 1727 29.02.2016 ru 0
This article presents information about the features of biotechnology as the driving force of scientific and technological progress. The national programs of the leading countries of the world, it is one of the priority sectors, reflecting the level of the socio-economic condition of the society. Biotechnology is now successfully solves such vital tasks as providing food, the establishment of effective medicaments, obtaining fuel based on renewable raw materials, maintaining ecological balance, conservation of biological resources of the Earth. The development of agriculture in modern conditions is impossible without agricultural biotechnology. It is directly related to viticulture. Choosing an object of an integrated system (embryos, apical meristem, axillary buds), it is possible to clone plants, i.e. produce plants identical to the original. If the same as the object to use isolated cells or protoplasts, in this case, there will most likely altered versions, creating diversity for the breeder. Genetic engineering – the science of younger, since the establishment of the first chimeric DNA molecule. The origin of genetic engineering is rooted in the development of molecular genetics, biochemistry. These technologies, undoubtedly progressive, but their biological safety is still insufficiently explored and is a danger to all life on Earth. The leading Western powers carried out strict control over the introduction of transgenic crop plants, as they are in agrocenosis new biological risks that may adversely affect the plants, animals and humans. In Russia, as in other countries, have already adopted the law “State regulation of genetic engineering”
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CHARACTERIZATION OF THE LIFE CYCLE OF A SCIENTIFIC ORGANIZATION

abstract 1562002009 issue 156 pp. 159 – 179 28.02.2020 ru 90
On the example of economic analysis, we have characterized the life cycle of a scientific institution, the history of the formation and development of which covers almost a three-hundred-year period. The article determines features of the methodology for identifying the stages of the life cycle of a scientific organization and identifying factors that determine the stage of the life cycle
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BIOTECHNOLOGY OF IMPROVEMENT AND PRESERVATION OF NATIVE DON GRAPE VARIETIES

abstract 1541910031 issue 154 pp. 327 – 347 30.12.2019 ru 114
The article presents the results of microclonal reproduction and recovery of native grape varieties, the creation of basic nursery. In addition to the culture of apical meristems, we have developed a chemotherapy using salicylic acid. A method of decontamination of plants from mycoplasma infection has also been developed, which includes the introduction of the antibiotic Cefotaxim in the nutrient environment at a concentration of 50 to 450 mg/L, depending on the degree of infection of plants. Adding to the nutrient medium of the drug Emistim reduces the death of meristems from infection by 3-5 times, improves their differentiation. The use of the drug Melafen helps to improve morphogenesis and quality characteristics of plants. The high survival rate of meristems during adaptation to non-sterile conditions has been noted. Biological testing on herbaceous indicators showed no chronic diseases. Plants after adapting to non-sterile conditions are planted, in the form of vegetative seedlings with a closed rootsystem, on the basic nursery. The survival rate of plants was 70-80%, in some varieties it is higher: 96.4% (Sypun black) - 98.6% (Krestovsky). It has been proved that in vitro clonal microbreeding there is no change at the genetic level even after 8 years of cultivation, which confirms the reliability of the developed technology of reproduction and recovery of native grape varieties in vitro
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DETECTED GRAPE VARIETIES OF AFGHANISTAN

abstract 1572003026 issue 157 pp. 346 – 367 31.03.2020 ru 122
The article reports in color on the characteristics of the three varieties and their clones that are common in Afghanistan. In the process of ampelographic screening of vineyards in Afghanistan, three varieties previously undescribed in Russian literature under the local names Aita, Gundyan and Shandyhani were discovered. Their identification by ampelographic and ampelometric characteristics made it possible to establish the original Aita variety, the Gundyan variety as a synonym for Guladan and Shandyhani as a synonym for the world famous Sultanin, or Kishmish. The described varieties are quite common in Afghanistan, reputable in economic returns and are still promising for cultivation in their country, where they are successfully used in the manufacture of dried products and high-quality diet juices. To identify the impact of various environmental conditions on the growth, development, quantity and quality of the crop, these varieties must be tested in all zones of different ecological and geographical areas of cultivation
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INFLUENCE OF FERTILIZERS ON QUALITY OF CHARDONNAY GRAPES AND WINE MATERIALS

abstract 1471903014 issue 147 pp. 53 – 61 29.03.2019 ru 184
The article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes
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DEVELOPMENT OF SIGNIFICANT PHENOTYPICAL TRAIT MODELS OF TABLE GRAPE

abstract 1401806018 issue 140 pp. 42 – 59 29.06.2018 ru 204
Intensive viticulture as a sub-branch of the grape-wine industry of agriculture is developing due to the introduction of modern scientific developments. The intensification of production consists of laying vineyards with planting material of higher biological categories, highly productive clones of traditional and long-established selection varieties; advanced agrotechnological techniques and mechanized operations, etc. For import substitution of table grapevine in our country, it is especially important to introduce new big-berry varieties from early to late maturity, providing a continuous 100-day conveyor for harvesting, with a bunch of grapes, various colors and the shape of berries. In many countries of the world with developed viticulture, breeding programs are being conducted to create competitive table varieties. As a result, there were obtained Muscat of Italy (Italy), Cardinal, Red Globe (USA), Moldova (Moldova), widely spread all over the world. But the world market does not stand still and breeders are working on new varieties. In recent years, there has been a trend of enlargement of berries from 8 to 24 grams, preference is given to varieties with pink and red berries. So, for example, created in Ukraine - a very early variety of Livia; in Russia - the variety clones of Livia K and Rochefort K, the early varieties of the Memory of the Teacher, the Gift of the Nesvetay, the Preobraghenie and the Jubilee of Novocherkassk, and others. These varieties are bred according to the developed model, which shows the genetic regularities of the formation of maximum expression in transgressive recombinants in quantitative (mass of berries and bunches) and qualitative (shape and color of berries) traits associated with maturation
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THE STUDY OF THE RANGE OF TECHNICAL GRADES OF GRAPES IN THE ALUSHTA VALLEY

abstract 1481904019 issue 148 pp. 142 – 152 30.04.2019 ru 212
The article reveals analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the branch "Alushta" of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon № 173 and № 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In General, the age structure of the vineyards is acceptable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions
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THE STUDY OF THE RANGE OF TECHNICAL GRADES OF GRAPES IN THE ALUSHTA VALLEY

abstract 1461902011 issue 146 pp. 38 – 48 28.02.2019 ru 226
The analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the "Alushta" branch of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon № 173 and № 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In general, the age structure of the vineyards is favorable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions
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THE QUALITY OF MERLOT GRAPE AND WINE ON A BACKGROUND OF APPLICATION OF MINERAL FERTILIZERS IN THE CONDITIONS OF ANAPA-TAMAN AREA

abstract 1301706090 issue 130 pp. 1236 – 1248 30.06.2017 ru 254
The article reviews the results of studying the influence of mineral fertilizers on the qualitative indices of the Merlot grape variety in the Anapo-Taman zone of the Krasnodar region. The technology of cultivation of grapes on the experimental plot was in accordance with the accepted for the given zone and culture. Agrobiological work was carried out at the optimal time and had a high quality of execution. Grape bushes are laid in a 3.0 x 2.0 m pattern. Formation - a twoshoulder horizontal cordon. On the bushes we formed the same load of shoots and bunches. Scheme of the experiment: option 1 – no fertilizers (control); option 2-superphosphate with a mixture of potassium salt (P90K90) from the autumn; option 3 –nitroammofoska (N120P120K120) from autumn; option 4 – ammonium nitrate (N60) in early spring. Analysis of the results of studies on the study of biological features of growth, fruiting, yield and quality of Merlot grape varieties on the background of the use of mineral fertilizers shows their high efficiency in the conditions of the AnapoTamanskaya zone of the Krasnodar region and can be recommended for use in production conditions. Mineral fertilizers ensures the receipt of dry bulk wine of superior quality control sample (without fertilizers) the concentration of tartaric acid 17.3 25.1 percent, phenolic compounds 17.1 – 40.0 %, and alcohol content of 7.2 and 9.3 %, titratable acids 11.8 and 13.7 %, given extract by 17.4 %, with a decrease of the mass concentration of total sulfur dioxide and pH. The most effective is the introduction of NPK (N120P120K120)
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SOME ASPECTS OF CREATION OF BASIC MOTHER LIQUIDS OF GRAPES IN THE CONDITIONS OF UST-KUNDRCHENSKY SANDY ARRAY

abstract 1351801012 issue 135 pp. 125 – 146 31.01.2018 ru 277
The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array
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