Scientific Journal of KubSAU

The article presents the results of the study of yield, mechanical and biochemical composition of Merlot grape vine variety and its two clones in the conditions of Taman and Anapa subzone of the Krasnodar region. Clones differ from the maternal variety by the large number of berries in the bunch, the mass of the berry, the average mass of the bunch and the yield. Maternal variety and its clones are characterized by very close percentages of berries and combs in the bunch and by indicators of the structure, composition and structure of the bunch. By the yield of juice percentage, in different growth areas clones showed the same results as the original variety. According to the content of glucose, fructose, organic acids (wine, apple, citric, succinic), potassium, sodium, magnesium and calcium cations in the juice of berries, there is no reliable differences between the original variety and its clones. The content of dry substances, sugars, titrated acids, and the sugar-acid coefficient depended on the yield, but in general, they were favorable for the production of wine materials and juices. Merlo phanagoriiskii and Merloc are ecologically plastic and belong to the first group of varieties that retain their valuable characteristics in different areas of growth

The article highlights the questions of preliminary determination of the yield of vineyards, grape harvest prediction method to determine the optimal length of fruit cutting of shoots and load bushes wintering eyes, kidneys examined productivity index wintering buds (CP), which shows how many inflorescences have an average for each left eye escape when cutting to length, including the dead. It was found that the yield of grapes has a great influence the degree of differentiation of inflorescence of rudiments in the central buds overwintering buds. From the moment of laying the harvest of grapes in hibernating little eyes in the form of embryonic inflorescences until commercial harvest takes two growing seasons. During this period the generative organs of grape plants are affected by many factors of the environment. Heterogeneity was found of buds along the length of one-year matured shoots. It is expressed in the fact that many grape varieties fruitfulness buds at the base of the shoot are much lower compared with upstream eyes. A certain death of wintering buds (primarily, the main kidney) at the end of the growing season, before the autumn frosts. The main reason for the death of buds during the growing season - from defeat fungal diseases. Taking into account the percentage of deaths of buds proposed a new exponent forming embryonic kidney main inflorescence - productivity index of buds by the ratio of embryonic stems to the number of buds, including the dead. Thus, annual forecasting of grape harvest next year to cutting the bushes by the proposed method makes it possible to scientifically establish the optimal cut length of shoots and fruit load on the bush eyes. This helps to realize directly the potential of each variety and vineyard site and get maximum yields even in years with low fruit tab formations

Two methods were developed, one to assess experimental results by reducing a number of developmental parameters to the overall quality, leading to the overall quality criterion, and the other to optimize processes affected by numerous interacting factors, in-vitro plant development in this case, by applying a mathematical design of experiment. Single-bud cuttings with one leaf of two Vitis vinifera L. genotypes were excised from the central part of two-month-old in-vitro grown plants and used as explants. The explants were established on bridges of filtering paper in liquid media and on solid media. Eighteen modifications of these media contained five macro-elements, each at three concentrations, and the distribution of these macro-element concentrations followed the law of random numbers (Experiment I). Parameters characterizing arm, leaf and root development of two-month-old plants of each study genotype established on each liquid or solid medium were reduced to the overall quality of plant development. Since the study genotypes differed in the ability to grow on liquid and solid media with different macro-element concentrations and also in the ability to utilize these macro-elements, the dependence of in-vitro plant development on macro-element concentrations was described by different regression equations. That is why the regression equation describing the average outcome of plant development in the two genotypes on liquid and solid media was not as significant and the description of the process was not as adequate (determined) as regression equations calculated for each process. The regression equation which describes the dependence of the average outcome of plant development on macro-element concentrations in Experiment I is as follows: y5 = 0.027 + 0.116x22 + 0.109x2x4 + 0.106x2x3 + 0.114x4. Stepwise calculation of macro-element concentrations to optimize in-vitro plant development was done based on macro-element concentrations of the initial medium of Experiment I as starting points since that medium was best efficient for this purpose by using regression equation (y5) and algorithms of multiple curvilinear stepwise regression according to the Box-Wilson method of steepest ascent. Experiment II was undertaken where macro-element concentrations (‘steps’) were calculated in a stepwise manner to optimize in-vitro plant development of the two V. vinifera genotypes and the rootstock ‘Kober 5BB’. This led to a liquid medium and a solid one which enabled a better plant development in the three genotypes relative to the use of controls: media with ½ MS macro-elements and the initial medium whose macro-element concentrations entered as starting points to calculate ‘steps’ for optimization of in-vitro plant development. The optimized medium contained macro-elements: 318 mg l-1 NH4NO3 (x1), 1188 mg l-1 KNO3 (x2), 370 mg l-1 MgSO4 7H20 (x3) (MS), 370 mg l-1 KH2PO4 (x4), 331 mg l-1 CaCl2 (x5) (MS), and other substances at optimum concentrations adjusted earlier: ¼ MS Fe-EDTA, ¼ MS micro-elements, 20 mg l-1 myo-inositol, 0.1 mg l-1 thiamine (MS), 0.5 mg l-1 nicotinic acid (MS), 0.2 mg l-1 pyridoxine, 2 mg l-1 glycine (MS), 0.1 mg l-1 indole-3-acetic acid, 10 g l-1 sucrose and, only for solid media, 7 g l-1 Difco agar. The optimized medium may be used for propagation of virus-free plants, valuable clones and grapevine genotypes created by gene engineering. The mathematical design of experiment reported in this paper which enables stepwise optimization of in-vitro plant development may be used both in agriculture and in the food industry

Nutrient media for shoot development from shoot tips 0.5-0.8 mm with several leaf primordia in four grapevine genotypes were optimized by means of a mathematical design of experiment. Over the range of component concentrations CaCl2 had considerable the highest effect on shoot tip development compared to other components of the media. Grapevine genotypes were different in their needs for concentrations of macro-elements to optimize the process of their growth and development. Shoot development from shoot tips in each grapevine genotype as a function of macro-element concentrations in media must be described by an individual regression equation. The proposed method of result evaluation and the mathematical design of experiment may be used in physiological and agricultural research for optimization of processes affected by numerous factors. The optimized media for shoot tip development may by used for sanitation of grape plants from viruses in meristem cultures

Numerous studies have found a different quality of the buds along the length of the fruit arms, which expressed various degrees establishing embryonic inflorescences in the central buds. It is revealed that many of the grapes have more fruit-bearing eyes formed on average (4-6 knots) and upper (7 to 10 knots) levels of fruit escape, compared to the lower (1-3 nodes) layer. In connection with the different quality of the buds along the length of fruit vines should be used and different ways of cutting them. It is necessary to consider the biological characteristics of varieties, ways of doing and shaping the bushes, the weather conditions of the area during the growing season and relative calm. To receive annually a stable high yield of grapes, we must first set the optimal length of pruning fruit shoots and optimal load on the Bush healthy eyes. This requires for each class on the eve of trimming bushes to carry out the analysis of the wintering holes along the length of the fruit shoots and to identify the embryonic fruitfulness of Central buds, i.e. to carry out the prediction of grape yield next year. The article presents data on the formation of the embryonic fruitfulness of Central buds of wintering buds at the Taman group of grapes – Arcadia, Viorica, Hibernal, Cabernet Sauvignon, Merlot, Moldova, Pinot white, Riesling, Saperavi, Traminer pink, Tsimlyansky black, Chardonnay. Found higher rates of embryonic fruitfulness of Central buds buds in the studied grape varieties - Chardonnay, Riesling, Viorica, Hibernal, Merlot, Moldova, where the coefficients of fruiting ranged from 1.40 m from Riesling to 1.69 from Merlot; factor productivity of 1.58 from the Chardonnay to 1.77 from Merlo. The high percentage of fruitful buds from 80,8 from Cabernet Sauvignon to 95.6 in the cultivars Merlot and Chardonnay. A higher percentage of eyes with 2 embryonic buds in the cultivars Merlot and Viorica who have this indicator amounted to, respectively: 75,6 and 70.6%. Forecasting a crop of grapes next year to trim the bushes on the proposed method will provide a full crop of grapes

The Patris variety is a hybrid, which was made as the result of Greece-Russian scientific collaboration in Greece by Panteley Zamanidi and Leonid Troshin in 2001 year by hybridization of Aigeorgitiko and Cabernet Sauvignon variety. By its morphological characteristics, it is included to the group of the Black sea varieties (convar. pontica subconvar. balcanica Negr.). The production period is 156-165 days. The growth of shoots is big. The yield is very high. The weight of bunch is 250 g. It has good frost resistance, drought resistance and higher steadiness to mushroom diseases, comparing with the parents varieties of Vitis vinifera L. The leave is middle, black-green, five lobes, deep divided, bubbles on the higher plate are low, the hairs are low on the upper side and intense on the bottom. The flower is hermaphrodite. The bunch is middle, cylinder conical or wrinkle, low density. The berry is middle, round, blue-black color. The skin is dense. The pulp is juicy with paslen taste. The sugariness of juice is high. The variety is used for making dry red wines of outstanding quality

Peculiarities of microсlone propagation of introduced varieties and grape clones, and home varieties created in Kuban State Agrarian University as well, which were given for national trial and for industrial production are discussed in this article.

In presence article results of the three-year study of new varieties of table grapes and elite forms of super-early and very early ripening by their Agrobiological and economically valuable characteristics in terms of the Crimea and North Caucasus.

Newest technical black berry variety named Piste (“Faith” in Greek) with colored flesh and juice breeded at Athens Institute of Viticulture (Greece) by researchers Zamanidi P., L. Troshin and P. Radchevskii in 2007 by crossing the Greek varieties Afoos (Mavrodafni x Alicante Boucher) with Ukrainian variety Odessa black (Alicante Bouchet x Cabernet Sauvignon). Duration of production period is 146-155 days. Growth of shoots is strong (2,1-3,0 m). The percentage of productive shoots is more than 90. The yield is very high: 25-30 t / ha. The average weight of clusters is 250 g. It can be characterized by high winter hardiness, drought resistance and increased resistance to fungal diseases in comparison with varieties of Vitis vinifera. Flower is hermaphrodite. Cluster is medium, conical, sometimes with wings, medium density. Berry is small, round, blue-black color with a thick waxy coating. The skin is medium thickness, dense and durable. Pulp and juice are intensely colored. Very high sugar content. Variety is intended for the production of intensely colored dry red wines of excellent class, as well as high-quality sparkling wines, dessert, sweet and liqueur wines; suitable for the manufacture of high-quality strong colored juices. When used in blends with other varieties enhances color, full flavor and enhances the bouquet of the wine

In the present article, we have described data of comparative ampelography of biometric evaluation of leaf parameters of the three table grapes: Preobragenie, Victor and Jubiley Novocherkassk, widespread in the amateur and farming areas of Russia and the Ukraine. Showed results of molecular genetic analysis of DNA from these table grapes