Scientific Journal of KubSAU

The article presents the material of forecasting for grape yield of next year and establishing the optimal loading if cutting of bushes. The material includes 14 varieties of grapes, 11 of them are technical and 3 are table ones. For each year of stable high yield of grapes, it is necessary to pre-set the optimum length of fruit cutting of shoots and optimum load on the bush healthy eyes. To do this for each variety on the eve of trimming bushes we perform optimum productivity analysis of wintering buds of fruit along the length of shoots, i.e. we implement forecasting of grape yield for next year. We have a plan of forecasting for yields of vineyards by microscopy of wintering buds on one-year shoots of fruit ripened grapes in order to establish the potential of embryonic establishment of inflorescences in the central holes of buds. Based on the analysis of buds, the indices were calculated for wintering fruiting buds and their degree of damage during the growing season. It was revealed, that the majority of grape varieties under study shows high tab embryonic inflorescences in central buds in overwintering buds for next year yield. Higher rates at a rate of fruiting buds were wintering in the varieties: Moldova (section 27). - 1.66; Bianca (section 6). - 1.83; Kunlean (section 15). - 1.71; Merlot (section 14). - 1.64; Saperavi (section 56). - 1.76. The lowest rates of fructification - the varieties Muscat Hamburg (section 21) and Augustine (section 11) and were respectively 1.20 and 1.24. As a planned productivity, we offered the optimal loading model of cutting bushes buds. As a result of productivity analyzes of buds along the length of the fruit shoots in 2016 we recommended to carry out pruning of fruit annual shoots 3-4 buds of the form of AZOS-1 and the form of cordon - 5-6 buds

The article highlights the issues of grape plants resistance to low temperatures; it is an urgent problem for all wine-growing regions of the world, located in the zone of risky agriculture. Losses caused by frost, indicate long-standing necessity of perfection of assortment in terms of enhancing its hardiness by enriching more resistant genotypes obtained both through the introduction or naturalization, as well as from the crossing of indigenous with interspecies hybrids. Methodology for evaluating frost resistance properties of the material served as a preparation for the originality of his research and experimental evaluation of the unique divisibility properties of the aim. Thus, we initially conducted hardening of matured cuttings at positive temperature plus 8 plus 4 ° C for 14 days (I quenching phase) and a negative temperature minus 5 ° C-minus 7 ° C for 9 days and minus 10 ° C-1 day (II hardening phase). Then the cuttings we frozen in the temperature range from minus 16 ° C to the next steps of consistent freezing (minus 16 ° C - 2 days; minus 18 ° C - 2 days; minus 20 ° C - 2 days; minus 22 ° C – 1day, minus 24 ° C - 1 day, minus 26 ° C - 1 day, minus 28 ° C - 1 day and minus 30 ° C - 8 hours). After freezing of cuttings of each variety, the sample was placed on the 3 days in the refrigerator at the temperature of + 2 ° C to their gradual thawing. Cuttings were grown at room temperature on water. As a result, obtained by the accounting: the lowest frost-resistance among the studied forms of parental Crimean origins was found in grades of Shabash, Soldayya and Solnechnodolinsky; the varieties of Dzevat kara, Kokur bely, Kokur cherny, Misket, Misgyuli kara, Sari Pandas were set as a weak level (-20 °C); Aybatly, Kefesiya, Kok pandas and Ekim kara have average level up to -22 °C; the highest frost-resistance - 24 °C – was shown by Khersonessky and Kapselsky. Also, we have identified synthetic frost-resistant F1-population and plus-transgressive selection form

The article gives the results of the study of the texture of the grapes and biochemical characteristics of red wine grape varieties of Cabernet Sauvignon, Cabernet Franc, Merlot, Sapeari, Tsimladar, Pinot Noir for the production of juice of direct extraction and blending. The studied varieties in terms of the structure of the cluster may be divided into three groups of blackpinene (21.1), Cabernet Franc and Tsimladar (18.7 - 17.5), Cabernet Sauvignon, Merlot, Saperavi (16.5 - 16.8) , and bunches of addition in terms of two groups - Tsimladar (17.6), Cabernet - Sauvignon and other (15.6 - 16.4). The structural component of grapes varieties ranged from 5.2 to 5.9, berry index - from 62.1 to 83.4, the output of the wort from 74.2 to 76.3%. The content of sugars and acids in the berries of all varieties is favorable for the production of beverages. The largest Tartaric acid content was found in the juice of the varieties of Cabernet Sauvignon, Pinot Black, malic acid - in the juice of varieties Saperavi, Merlot, Tsimladar, citric acid - in the juice Tsimladar varieties, Cabernet Sauvignon, Saperavi. The ratio of tartaric acid to the content of the apple juice Cabernet Sauvignon was 2.9: 1, Cabernet Franc 2.6: 1, Pinot Black 2.5: 1, Merlot and Tsimladar 1.6: 1, 1.1 Sapevari: 1. According to the content of potassium cations, sodium, magnesium and calcium there were chosen three varieties of Cabernet Sauvignon and Cabernet Franc. The lowest content of potassium and magnesium cations was in the variety of Tsimladar, Sodium – in the varieties Merlot and Saperavi, calcium – in Saperavi. Excellent tasting commended for juices was given to Cabernet Sauvignon, Saperavi, Tsimladar, good – to Cabernet Franc, Merlot, Pinot black. Best blending juice obtained by mixing the juice of the grape of the varieties of Cabernet Sauvignon and Pinot black 70:30, Saperavi and Pinot black 50:50. From the grapes of produced varieties, we can produce juice and varietal by name ampelographic variety and blended to extend the range

The article presents the results of studies of 92 indigenous varieties and 15 wild genotypes by 24 microsatellite markers. As a result a locus database about germplasm in the collection stored at the Russian ampelographical collection AZOSViV (Anapa Zone Experimental Station of Viticulture and winemaking) was created. During the research of varietal breeding populations confirmed the validity in the production of the cultivar of Krasnostop anapsky with obvious changes in microsatellite sequences as a clone of famous indigenous Don cultivar Krasnostop Zolotovskii. This clone was selected in 1953 by prominent agrotechnologist Zotkin Ivan Ivanovich which is propagated by the author and his followers, and after a formal registration submitted to state testing in 2007 in GSK RF, zoned in the North Caucasus region (number 6) in 2014 year. Sort -clone Krasnostop anapsky only in the Kuban today cultivating in 35 hectares. By using molecular genetic techniques, now clonal selection of vine grapes is reasonably improving. Recent data collected by us in the work process, were analyzed a comprehensive manner, making it possible to draw conclusions about distinctness of grape varieties. Some clones were studied by DNA primers, based on retrotransposon sequences

It is well known that genetics studies the mechanisms of variation/heredity and widely uses the concept of "genetic information". While genetics considers the information as the content of the genetic code - structure of DNA and RNA included in the cell of a living organism. Genetics examines the mechanisms of recording, copying, readout of genetic information, the possibility of its modification and its influence on the characteristics and properties of the organism. In conversational and scientific language we know phrases, such as "Genes contain information about the characteristics/properties of the body." Paradoxically, we see no attempts to determine the amount of information contained in specific genes on specific characteristics or phenotypic properties of the organism. It would seem that the application of information theory in genetics is a completely natural and suggests itself. More strange that there are practically no works devoted to the application of information theory for solving problems of genetics. This article is intended, to some extent, to fill this gap on the example of calculating the amount of information in the genes of the characteristics or properties of different grape varieties. It examines the application of automated system-cognitive analysis (ASC-analysis), its mathematical model – system of information theory and software tools – intellectual system called "Eidos" for solving one of the important tasks of genetics: determine the amount of information contained in the genes on various phenotypic characteristics/properties of the grapes. To solve this problem, we perform the following steps: 1) cognitive-targeted structuring of the subject area; 2) the formalization of the subject area, i.e. development of classification and descriptive dials and graduations and training samples; 3) synthesis and verification of information model, reflecting the amount of information in the genes on the phenotypic characteristics/properties (multiparameter typing); 4) displaying the information about the genetic determination system of phenotypic characteristics/properties (SWOT analysis of Fennovoima); 5) displaying the information about the strength and direction of influence of a specific gene on phenotypic characteristics/properties (SWOT-diagrams of genes); 6) the solution to the problem of system identification phenotypic characteristics/properties by the presence of certain genes; 7) quantification of the similarities-differences of the various phenotypic characteristics/properties, upon determination system genes. A specific phenotypic property (or characteristic) is regarded as a noisy genetic text, including genetic information about the true gene property (clean signal) and the noise that distorts this information due to the random effects of the environment. The software tool of the ask-analysis which is "Eidos" intellectual system provides the noise suppression and the selection of true signal

The article presents the data on the formation of the embryonic fruitfulness of central ovaries of wintering buds of the group of technical grape varieties with white berries - White Muscat; Pinot White, Chardonnay, Citron Magaracha, Pervenets Magaracha; Riesling, Viorica, Riton, Crystal in the conditions of Anapa-Taman zone. There were revealed the rates of embryonic fruitfulness of central ovaries of buds of studied cultivars and fruiting indices of vegetative shoots developed from them. In all studied grape varieties there was revealed a high percentage of fruitful buds from 84,1 in the variety of Riton at 97.2 at Viorica; and the percentage of fertile vegetative shoots from 81,8 at the variety Citron Magaracha to 97.2 in the variety White Muscat. At the leveled load of bushes, vegetative shoots and the same scheme of planting of bushes (3 x 2 m), the highest yield in terms per hectare showed the varieties Pervenets Magaracha, Viorica, Riton, Crystal, Riesling and Citron Magarach. When assessing the economic efficiency the highest net income and level of profitability were identified in the varieties of Citron Magaracha, Pervenets Magaracha, Viorica and Riton. In order to determine which buds will give us shoots with large, well-developed (well-differentiated) buds, and which will not give (weakly differentiated), it is necessary "to look inside a bud". But even already formed germs of inflorescences in the bud are able in a few days in spring or dedifferentiate or degrade depending on the influence of external conditions. Scientists have learned to use this ability to increase the maximum possible yield in years of severe damage of grape by frosts. Firstly, in frosty winters the central buds wither out. The replacing buds usually have poor fruiting and bad productivity during years. In such cases, it is more profitable to conduct a small cutting of angle buds arranged in a circle at the base of a shoot. At the beginning of the second vegetation phase, these buds will obtain the great bulk plastic substances, which will cause the dedifferentiation of rudiments of inflorescences in them. Thus, it is possible not only to restore rapidly the normal shape of a bush, but to obtain a good harvest this year

Clonal micro-multiplication – is this the basic method of obtaining the qualitative virus-free planting material. The high quality specificity of the explants reaction of the grapes varieties to the composition of nutrient medium requires the individual selection of the medium components for the most successful multiplication in vitro. In the article we present the results of studies on the cultivation of the grapes apexes in vitro the modified medium with the reduced content of macrocells. As a result of conducted investigations we have established that for the grapes varieties of Academic Trubilin, Artemis, Gurman Kraynova, Maria Kallas, Nizina, Petit Verdo and Traminer Black the most effective introduction into the in vitro culture (acclimatization of apexes 80- 100%) occurs on modified nutrient medium to Murasige and Skoog (1962), by being differed in terms of the content macro-elements (mG/l): NH4NO3 – 1237; KNO3 – 1425; MgSO4 · 7N2O – 277,5; KN2PO4 · H2O – 277,5, of the vitamin Of v1 – 10,0 mG/l, nicotinic acid – 4 mG/l. Remaining varieties of Kishmish luchistiy, Preobrajenie, Roshfor K and Yubiley Novocherkasska in the same medium were developed slowly and in a different way

In this article we present information on features of microcloning in culture of in vitro of perspective for Eurasia, pink and white-berry table genetic grades of grapes by I. A. Kostrikin, V. N. Kraynov and V. V. Zagorulko. Researches were conducted at an input stage in culture of in vitro of meristems of 0,1-0,2 mm in size. At the majority of grades and forms, we noted good regeneration abilities in the conditions of sterile culture. It is bound to the fact that almost all the studied grades: Anyuta, Bogatyanovsky, the Princess Olga, Preobrazhenie, Anniversary of Novocherkassk, Favor are received from crossing of couple the Mascot × Sultana-ray grape. And only grades called Libya and Lowland which showed lower regeneration properties at stages of proliferation and rooting of shoots are received from crossing the couple of Flamingo × Arkadya and (Talisman x Tomaysky) respectively. At the same time, the grade of Talisman, being one of the parents of the majority of the studied grades, showed stable results at all stages of cultivation

Numerous studies have found a different quality of the buds along the length of the fruit arms, which expressed various degrees establishing embryonic inflorescences in the central buds. It is revealed that many of the grapes have more fruit-bearing eyes formed on average (4-6 knots) and upper (7 to 10 knots) levels of fruit escape, compared to the lower (1-3 nodes) layer. In connection with the different quality of the buds along the length of fruit vines should be used and different ways of cutting them. It is necessary to consider the biological characteristics of varieties, ways of doing and shaping the bushes, the weather conditions of the area during the growing season and relative calm. To receive annually a stable high yield of grapes, we must first set the optimal length of pruning fruit shoots and optimal load on the Bush healthy eyes. This requires for each class on the eve of trimming bushes to carry out the analysis of the wintering holes along the length of the fruit shoots and to identify the embryonic fruitfulness of Central buds, i.e. to carry out the prediction of grape yield next year. The article presents data on the formation of the embryonic fruitfulness of Central buds of wintering buds at the Taman group of grapes – Arcadia, Viorica, Hibernal, Cabernet Sauvignon, Merlot, Moldova, Pinot white, Riesling, Saperavi, Traminer pink, Tsimlyansky black, Chardonnay. Found higher rates of embryonic fruitfulness of Central buds buds in the studied grape varieties - Chardonnay, Riesling, Viorica, Hibernal, Merlot, Moldova, where the coefficients of fruiting ranged from 1.40 m from Riesling to 1.69 from Merlot; factor productivity of 1.58 from the Chardonnay to 1.77 from Merlo. The high percentage of fruitful buds from 80,8 from Cabernet Sauvignon to 95.6 in the cultivars Merlot and Chardonnay. A higher percentage of eyes with 2 embryonic buds in the cultivars Merlot and Viorica who have this indicator amounted to, respectively: 75,6 and 70.6%. Forecasting a crop of grapes next year to trim the bushes on the proposed method will provide a full crop of grapes

The article presents results of the study of yield, mechanical composition and quality of bunches of newest white grapevine varieties called Artemis, Sauvignon tamanskii and Inkroche tamanskii, in the conditions of Taman in the Krasnodar region. The yield of the studied genotypes ranged from 10.4 to 13.3 t / ha: the highest yield was in Artemis, and the lowest – in Sauvignon tamanskii. The average weight of the bunch was in Sauvignon tamanskii (152 g), the variety Artemis had 143 g and Inkroche tamanskii had 130 g of bunch weight. The percentage of berries in clusters ranged from 95.3 in Artemis to 95.9 in Inkroche tamanskii and Sauvignon tamanskii. The ratio of fruit mass to the mass of crests was 20.3 in the varieties of Artemis and 23.5 in Inkroche tamanskii and Sauvignon tamanskii. In genotypes of Artemis, Sauvignon and Inkroche Taman Taman the weight of 100 berries was 200, 160 and 130 g, respectively, the mass of 100 seeds - 4.6; 3.1 and 2.9 g, the average weight of the pulp from the juice to the weight of the skin - 24.3; 19.5; 15.9. The percentage of pulp and juice in clusters of the studied varieties was 85,9-89,1%, and the ratio of pulp to juice to the solid residue 6,1-8,2. Yield of musts from the studied sample was 74,5-75,4%. The content of sugars in the berries (17,5-19,6 g / 100 cm3) was dependent on the yield, the characteristics of varieties and timing of the harvest, but the harvest was favorable for the production of beverages and wine materials