Scientific Journal of KubSAU

The article discusses the use of automatic systemic-cognitive analysis (ASC-analysis), its mathematical model is a system of information theory and software tools – an intellectual system called "Eidos" for the solution of some problems of ampelography: 1) digitization of scanned images of the leaves and creation of their mathematical models; 2) the formation of mathematical models of specific leaves using the spreading of information theory; 3) the formation of models of generalized images of leaves of various sorts; 4) comparing an image of a specific leaf with a generalized image of the leaf of different varieties and finding a quantitative degree of similarity and differences between them, i.e. the identification of the varieties on the leaf; 5) quantification of the similarities and differences of the varieties, i.e. cluster-constructive analysis of generalized images of the leaves of different varieties. We propose a new approach to digitizing images of leaves, based on using the polar coordinate system, the center of gravity of the image and its external contour. Before scanning images we may use transformation to standardize the position of the still images, their sizes and rotation angle. Therefore, the results of digitization and ASC-analysis of the images might be invariant (independent) relatively to their position, size and rotation. The specific shape of the contour of the leaf is regarded as noise information on the variety, including information about the true shape of the leaf of the class (clean signal) and noise, which distort this true form, originating in a random environment. Software tools of ASC-analysis – intellectual "Eidos" system ensures noise reduction and the selection of the signal about the true shape of the leaf of each variety on the basis of a number of noisy concrete examples of the leaves of this variety. This creates a one way form of a leaf of each class, free from their concrete implementations, i.e., the "Eidos" of these images (in the sense of Plato) is a prototype or archetype (in the Jungian sense) of the images

Vitis vinifera subsp. silvestris Gmel. is the wild subspecies of Vitis vinifera L. and an important source of genetic resources for grapes breeding. Currently, this form is very rare in the world. In this paper, conducted the first study of wild grapes which was found in the North Caucasus with the use of two microsatellite markers. The study have shown a high level of genetic diversity: intrapopulation variability was 75,2%, the interpopulation variability accounts was 24,8%. All populations are characterized by certain unique genetic structure and may be as donor of resistance genes which have a long-term evolutionary selection

In the article we have studied 55 aboriginal varieties, collected in the North Caucasus. We have described allelic diversity and identified four haplotypes. All the populations were closely related while being examined using a factor of Nei's genetic distance (Dn), with the exception of indigenous varieties of Armenia. Maximum differences have been found between the Uzbek population and Astrakhan, Turkmenian, Armenian populations. The most genetically rich were the population of Don area and Krasnodar region

The article reviews the results of studying the influence of mineral fertilizers on the qualitative indices of the Merlot grape variety in the Anapo-Taman zone of the Krasnodar region. The technology of cultivation of grapes on the experimental plot was in accordance with the accepted for the given zone and culture. Agrobiological work was carried out at the optimal time and had a high quality of execution. Grape bushes are laid in a 3.0 x 2.0 m pattern. Formation - a twoshoulder horizontal cordon. On the bushes we formed the same load of shoots and bunches. Scheme of the experiment: option 1 – no fertilizers (control); option 2-superphosphate with a mixture of potassium salt (P90K90) from the autumn; option 3 –nitroammofoska (N120P120K120) from autumn; option 4 – ammonium nitrate (N60) in early spring. Analysis of the results of studies on the study of biological features of growth, fruiting, yield and quality of Merlot grape varieties on the background of the use of mineral fertilizers shows their high efficiency in the conditions of the AnapoTamanskaya zone of the Krasnodar region and can be recommended for use in production conditions. Mineral fertilizers ensures the receipt of dry bulk wine of superior quality control sample (without fertilizers) the concentration of tartaric acid 17.3 25.1 percent, phenolic compounds 17.1 – 40.0 %, and alcohol content of 7.2 and 9.3 %, titratable acids 11.8 and 13.7 %, given extract by 17.4 %, with a decrease of the mass concentration of total sulfur dioxide and pH. The most effective is the introduction of NPK (N120P120K120)

The newest wine white-berry fruitful high-grade breed of grape - Pontos is bred in Greece P.Zamanidi and L.Troshin in 2003 by mating of the Greek breed of Sideritis with a breed of Sovignon. Duration of productivity phase is 156-165 days. A breed is tall. A degree of vines ripening is very high. Productivity is very high, 15-16 t/hectares and more. The medial mass of racemations is 390 gr. Differs with high winter-hardiness, drought resistance and raised sustainability to fungous diseases in comparison with breeds Vitis vinifera. A flower is bisexual. Racemations are big, pencil-point, with medial denseness. Baccas are average, circular, greenish-yellow color, with a dense wax coating. A peel is tight. Pulp and juice with the expressed high quality flavor. Sugar content is high. It dries when over-ripening and stays on bushes. Under morpho-physiological characteristics, it is included in ecological-geographical bunch of breeds of basin of Black sea. The breed is designed for production of dry white wines of the excellent class, and also high-grade sparkling wines; it is applicable for production of high-grade juice and compotes as well

The analysis of genetic polymorphisms of 12 autochthonous grape varieties grown in the National ampelographic collection of Russia (Anapa district of the Krasnodar region) through the study of allelic diversity at six microsatellite loci: VRZAG79, VVMD5, VVMD7, VVMD27, VRZAG62, VVS2 has been done. We have found that all native varieties have a unique set of allele. The assessment of genetic relationships varieties has been performed using cluster analysis. Data for DNA certification of the investigated genotypes of the grapes has also been obtained in the article

Brandy alcohols from the grades of Muscat Berkatu, Kakhet and mixes of white grades of grapes became objects of researches. The content of some aromatic components of wine alcohols was investigated using the device of a gas-liquid chromatography Clarus-400 (with the ardent and ionization detector, a capillary column, 60 m x 0,32 mm, Elite-WAX ETR, gas-carrier-helium). Muscat alcohol according to the general content of aromatic substances (919,35 mg / 100 ml a.a.) was different from the other studied samples at the expense of the high content of ethyl acetate and izoamyl alcohol. As a result of the organoleptic analysis of a blend samples it was allocated a brandy blend with the following ratios of alcohol and wine - a blend alcohol – 74,37 %, liqueur wine – 25,63 % with strong muscat aroma and shades of taste

The article presents the structural and phylogenetic analysis of VIT_02s0033g00410, VIT_02s0033g00390 and VIT_02s0033g00450 genes of the grapevine genome and the closely related orthologous gene MYB114 of the Arabidopsis genome. These genes are responsible for the biosynthesis of anthocyanin in the organs of model plants and are of interest not only for practical production and breeding, but also for fundamental research. These genes were analyzed for GCcomposition of nucleotides, the presence of cisregulatory elements and promoter regions. DNA and protein sequences were aligned to look for similar elements, which allowed further analysis of the ultraconservative domains of four genes. Based on the results of search and identification of the conservative regions, a cluster tree was constructed, which made it possible to identify the separation of gene sidelines from, presumably, the main one. At the same time, the construction of consensus trees based on DNA and protein sequences revealed their absolute similarity. "The Minimal Evolution Tree" allowed calculating the approximate dates of the appearance of the mutations and the divergence times of the gene branches between each other. At the same time, the appearance of the Vitis genus and its separation from the Rosales was taken as the time first divergence point. In the end, homologous metabolic pathways were searched between grapevine and Arabidopsis, which revealed the presence of homologous proteins in the grape proteome. In this turn, it already confirms the existence of similar biosynthetic pathways and, as a consequence, interactions such as "DNA-protein" and "protein-protein"

Steady in a complex seedless Athena sort is born in Greece by Panteley Zamanidi and Leonid Troshin in 2001 by crossing of Sidiritis and Cabernet-Sauvignon sorts. Under morphological and physiological characteristics it is close to the group of Convar. pontica subconvar balcanica Negr. varieties of pool of Black sea. Duration of productional period is 156-165 days. Growth of arms is strong. Degree of ripening of a rod is high. Productivity is high. Percent of fructiferous arms is 90. The average weight of clusters is 400. It differs with high winter hardiness, drought resistance and raised stability to mushroom illnesses in comparison with seedless Euroasian grapes. A blossom bud has green color with yellowy-brown tones. A top of young arm of green color with a weak wine-red border and very strong web-like fluff. Young arm is red from the back, green with a belly with an average web-like fluff. Adult leaf is symmetric. Leaf plate is average or big, green color, five bladed, it is divided weakly. Both parts of a local teeth form straight lines. The form of petiolar dredging is open. Length of footstalk is average. One arm usually has two inflorescences, on 4th and 6th knots. Inflorescences are formed both on the arms which have grown from replacing buds, and from the arms which have been developed from sleeping buds on a long-term wood. The flower is hermaphroditic. Custer is large, branchy, winged, average density. Berry of the average size, roundish, blue and black color. Skin is thin, strong. Pulp is juicy, with solanaceous taste. Sugar content is high. Rudiments of seeds are small. The sort is intended for consumption in a natural way and for manufacturing dry red wines of various categories

The generalized long-term experience on creation of base of Queen cells in grape varieties, improved in vitro culture, on the sandy array of Ust-Donetsk district of Rostov region. Problems frequently encountered on sandy soils and ways of their solution are shown. We focus on: rehabilitation of the landing of plants of grapes in the conditions of greenhouses, open ground, the optimum time and methods of planting. The work shows the effectiveness of different dressings and types of fertilizers, influence of drugs of new generation, affected the uterine protection of plantations from pests and disease, deep sandy soils freezing in winter, especially the development of root system of different varieties. On the basis of generalization of long-term data, we suggest the most appropriate solutions, and developed technology bookmarks and conducting basic queen cells from healthy in vitro planting material of grapes in the sandy array