Scientific Journal of KubSAU

Vitis vinifera subsp. silvestris Gmel. is the wild subspecies of Vitis vinifera L. and an important source of genetic resources for grapes breeding. Currently, this form is very rare in the world. In this paper, conducted the first study of wild grapes which was found in the North Caucasus with the use of two microsatellite markers. The study have shown a high level of genetic diversity: intrapopulation variability was 75,2%, the interpopulation variability accounts was 24,8%. All populations are characterized by certain unique genetic structure and may be as donor of resistance genes which have a long-term evolutionary selection

In the article the questions of grape plantings spareness elimination by the ways of filling-up saplings with cuttings of scleroticе rods, green arms or a bush and reinoculation of bushes. With the preventive purpose, for reduction of plantings spareness degree on a vineyard, stamping of arms, application of potash fertilizers in the summer and in the autumn, stopping of watering in due time, normal loading of bushes with a crop and harvesting in due time are done, i.e. all the conditions for the best ripening of a rod and preparation of plants for winter are made

In this innovational article the results of researches of influence of "RADIKS" - the new-in-Russia root stimulator on reclaiming properties of grape cuttings, its various concentration, an amount and quality of vegetative, own-rooted and imparted saplings of various grapes, and also the results of working out of regulations of application of this preparation are presented

In innovational section the results of researches of application of “RADIKS” biologically active substance are presented at prelanding processing of cuttings and desktop inoculations on an amount and quality of own-rooted, imparted and vegetative grapes saplings

In this innovational article the results of researches of the influence of new-in-grape nursery-garden practice of Russia GUMINA preparations - LIGNOGUMAT KALIUM (GUMAT KALIUM) with microelements and GUMAT KALIUM LIQUID PEAT - on reclaiming properties of grape cuttings, amount and quality of own-rooted and imparted saplings of various grapes varieties are presented

Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA

In this innovational section the results of two-year test of technology of influence of biologically active substances "Rostock", "Kremniy", «Lignogumat kaliya» and "Zircon", and also water activated through the matrixes of these substances by means of "Akvator" device grape plants of Bianka variety are presented. Researches have shown, that the water activated by "Akvator" device, possesses surprising abilities, its influence on a grape plant exceeds influence of BAS solutions more than in 1,3 times by efficiency

In the article we have studied 55 aboriginal varieties, collected in the North Caucasus. We have described allelic diversity and identified four haplotypes. All the populations were closely related while being examined using a factor of Nei's genetic distance (Dn), with the exception of indigenous varieties of Armenia. Maximum differences have been found between the Uzbek population and Astrakhan, Turkmenian, Armenian populations. The most genetically rich were the population of Don area and Krasnodar region

In the given article, innovations of application of Nutrivant-plus spray feeding at Viorika variety vineyards are presented. As a result, it has made more considerable impact on the basic agro biological and technological indicators of a variety, than monokalium phosphate standard fertilizer; it has promoted quantity increase of embryonic inflorescences and their differentiation; stimulated an increase in a weight of a cluster and, as a consequence, increase of a crop from a bush and hectare; considerably accelerated and stimulated process of accumulation of sugars in juice of berries; led to increase in the area of a sheet plate; provided increase of economic efficiency of manufacture of grapes

This article features the recommendations on microclonal grapes reproduction at all stages of their realization