Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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№ 143(9), November, 2018

Date issued: 30.11.2018

Life Sciences
188 kb

SEED PRODUCTION AND TECHNOLOGICAL METHODS OF PRODUCTION OF SUGAR BEET SEEDS IN THE KRASNODAR REGION

abstract 1431809011 issue 143 pp. 1 – 15 30.11.2018 ru 920
The article discusses the organizational scheme of sugar beet seed production, as well as technological methods of seed production using the stick method and the cultivation of basic seeds in NRU. We substantiate the key elements of the cultivation of uterine sugar beet of summer sowing season
429 kb

A MODIFIED PROTOCOL OF RNA ISOLATION FROM MATURE LEAVES OF GRAPES FOR RT-PCR

abstract 1431809012 issue 143 pp. 16 – 30 30.11.2018 ru 492
Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
162 kb

CLONALE MICROPROPOGATION AND SANITATION OF PRUNUS DOMESTICA FROM THE PLUM POX POTYVIRUS (PPV)

abstract 1431809013 issue 143 pp. 31 – 40 30.11.2018 ru 645
In the article we present the analysis of the results of studies of russian, foreign scientists, as well as our own data, obtained in the clonal micro-multiplication and sanitation of the plum domestic from Sharkey's virus (PPV). The urgency of work is determined by the fact that the plum is the second on the significance culture in horticulture of the south of Russia. One of the most economically significant objects of virus etiology on the discharge of relative is considered to be Sharkey's virus of plum (Plum pox potyvirus). In the Krasnodar region and in the Russian Federation for the first time, Sharkey's virus of plum was discovered in the end of the 1980th, after which it widely extended into other regions of the country. In the complex of the methods of sanitation from Sharkey's virus of plum we successfully use thermo- and chemotherapy in combination with the method of apical meristem. The mechanism of the formation of virus-free meristem consists of the delay of the process of the replication of virus particles from the rapid, anticipating increase in the rudimentary cloths and organs, especially, if plant undergoes by thermo- or chemotherapy. As virucide there are used the preparations neo -DHt (85 mG/l), and also salycilic acid in the concentration of 3kh10-4 M with the simultaneous magnetic-pulse working of mericlons. As the most suitable nutrient medium for the micro-multiplication of plum we considered Murasige- Skoog medium, on basis of which we have prepared different modifications. As the growth factors, which increase the effectiveness of multiplication, we used 6-BAP in the concentration 0,5-1 mG/l (in the stage of introduction into the culture and the stage of animated cartoon). For the improvement in the quality of the micro-shoots of plum, additionally to the growth factors, one should apply succinic acid, succinates of potassium and sodium in the concentration 4 mG/l. Obtained in the course of clonal micro-multiplication and adapted mericlons will be tested to the virus carrying ability and will be approved according to the quality signs. The ovaries of initial plants are embedded by healthy quality seedlings
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INHERITANCE OF PRODUCTIVITY TRAITS IN RICE HYBRIDS

abstract 1431809014 issue 143 pp. 41 – 48 30.11.2018 ru 280
Great success in increasing rice production, and it has more than doubled, has occurred worldwide thanks to the use of modern high-yielding rice varieties developed by conventional breeding methods. In order to develop a new variety, it is important for the breeder to study the genetic and breeding value of various cultural and wild species - carriers of economically valuable traits, the identification of genes and groups of genes controlling the economically valuable traits, to study the patterns of their inheritance, to establish patterns of interaction between the genotype and the environment, explore the genetic and physiological-biochemical basis of the phenomenon of heterosis, improve methods of intraspecific, complex, stepwise and distant hybridization, to study a phenomenon of a sharp increase in the quantitative characteristics of crop plants, a more powerful growth of hybrids of the first generation compared with the parent individuals. Scientists of the past centuries, such as A.F. Wigman, W.Noden, V.Foke, and many others have been studying the phenomenon of hybrid power, which was first named “heterosis” in 1908 by J. Schell. At present, heterosis is understood as the phenomenon when hybrids of the first generation exceed their parental forms in terms of their biological values and economically valuable traits. The magnitude of heterosis of the first generation hybrids is determined by several methods in terms of percentages to the paternal, to the maternal form, to the average of both parents, and to a better or regionalized standard. By the magnitude of the dominance coefficient, researchers often determine the inheritance of a trait
Life Sciences
3720 kb

MATHEMATICAL AND NUMERICAL MODELING OF THE RELATIONSHIP BETWEEN MORPHOLOGICAL, BIOCHEMICAL AND TRACE ELEMENT COMPOSITION OF BLOOD OF HEREFORD BREED CALVES AND THEIR SIZE

abstract 1431809033 issue 143 pp. 49 – 88 30.11.2018 ru 658
The researchers obtained data on the morphological, biochemical and trace element composition of the blood of bull-calves of Hereford breed of different sizes. In this regard, scientists and business executives have three natural questions: 1) whether it is possible to predict the size and thus the meat productivity of bulls using these blood indicators; what are the strength and direction of the influence of certain values of blood indicators on the size and weight of bulls; what blood indicators are similar in meaning, and what are different and how much (to what extent). The article is devoted to the reasoned answers to these questions by applying modern methods of mathematical and numerical modeling to solve the corresponding problems. The results of the study can be used by anyone, due to the fact that Eidos the universal automated system, which is a tool of ask-analysis, is in full open free access on the author's website at: http://lc.kubagro.ru/aidos/_Aidos-X.htm, and numerical examples of solving the mentioned problems with the use of artificial intelligence technologies are placed as a cloud Eidos-application #133
.