Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Коnоnеnко Sergey Ivanovich

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Kuban State Agrarian University
    North-Caucasus Research Institute of Animal

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Articles count: 32

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abstract 1341710034 issue 134 pp. 404 – 426 29.12.2017 ru 1393
A method of obtaining insertion mutants for the hoc gene, which encodes for the main phage antigen, was developed on the model of bacteriophage T4. This gene was cloned in the plasmid pBSL0+ and was disrupted by insertion of foreign DNA. The phage mutants were obtained by in vivo phage-plasmid recombination. The construction of insertion bacteriophage mutants was carried out in two stages. The resulting mutants on this procedure could be grown on wild-type E. coli strains, which is convenient for the production and use of these phages in therapy. The mutants obtained had reduced antigenicity. At the same time, the yield of the mutant strains was high when they were grown on the non-suppressor E. coli laboratory strains. A number of stages of purification of the bacteriophage mutants obtained were performed. Preparations have been studied by transmission electron microscopy and mass spectrometry. By several periodic cultivations of the mutant bacteriophages, it was shown that mutations of this type are stably maintained during more than 50 generations. T4 related bacteriophages of the family Myoviridae, for example, T-even, have the significant homology amongst their genomes, which makes possible to produce similar mutants. Thus, our method was developed to obtain mutants with reduced antigenicity which can be used for both the treatment of systemic infections, and diarrhea in the case, when, bacteriophages penetrate into the bloodstream. Such phages can be used in medicine and veterinary. The reported study was partially supported by RFBR, research projects No. 13-04-00991, 16-44-230855
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abstract 1361802030 issue 136 pp. 189 – 200 28.02.2018 ru 457
The main diseases of lactating cows occur in the first two months of lactation, i.e. during the production peak of productivity. They are caused by the changes in metabolism during the transition period, which are not supported by adequate changes in the organization of feeding, or rather sufficient to ensure cows with nutrients, which causes a number of closely related diseases. To allocate any of them is not possible, as their manifestation depends on a number of reasons, and depending on situation may prevail one or the other. Among the most common diseases, there are ketosis, milk fever (paresis of maternity), dysplasia of the abomasum, acidosis, mastitis, endometritis, laminitis and leukemia. These diseases cause the greatest problems in herds with high productivity, and are caused by changes in metabolism in cows in the transition period and the inability to properly feed the animals at this time. To identify the causes of these diseases we do not need to study each of them individually - it is enough to deal with the peculiarities of metabolism. This period includes the month before calving and the first or second month of lactation, but the most important are 3 weeks before calving and 3 weeks later. In order to prevent metabolic disorders there were developed and tested feed for feeding cows before calving and immediately after calving. Their effect was studied on milk production and health status