Name
Suprun Ivan Ivanovich
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
All-Russian Research Institute of Rice
Web site url
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Articles count: 22
In the presented study, we have performed genotyping
of modern Russian rice cultivars using microsatellite
DNA-markers. The markers showed different level of
allelic polymorphism: from 2 to 8 alleles per locus. For
all studied cultivars,unique DNA-fingerprints were
obtained
The wide variety of microorganisms has been identified in many wine-making countries on the berries of grapes. These are yeasts of different families, forms and kinds, bacterium, mold fungi. In the article, we present the results of investigating species composition of microflora of berries of white and red types of grape, which grows in different economies of the Krasnodar region and the republic of Abkhaziya. The sowings onto the elective media were conducted for the development of entire spectrum of yeast. The grown colonies after preliminary microscoping were separated into the cultures and subjected to testing according to the culturalmorphological signs, being guided by determinants and benefits. It was established the specific variety of microflora on the surface of the berries of grapes of all investigated types, without dependence on the place of their growth. Obtained data showed that the group of yeast, which constantly is present in the complex of the epiphytic microorganisms of grapes of Saccharomyces, Pichia, Hansenula, Hanseniaspora was characteristic for all types of grapes in all investigated regions. The heterogeneity of the taxonometric composition of microflora is shown. Prevailed yeasts were of family Saccharomycetaceae, form Saccharomyces vini. A quantity of yeast of Saccharomyces vini decreases in a number of Myskhako-Caucasus-Fanagoriya, that as a whole will be coordinated with the climatic conditions. Only the type of Pinot nuar grapes had yeasts of Brettanomyces Dekkera. On the berries of Cabernets and Karaburnu we have discovered yeasts of Schisosaccharomyces acidodevoratus, causing acid-reduction. On the berries of the grapes, which grew in joint stock company APF “Fanagoriya” we haven’t revealed the presence of lactic acid bacteria Lactobacillus brevis and yeasts of the form of Schisosaccharomyces acidodevoratus. In the same farm the smallest quantity of yeastswreckers is noted, which we the forms of Pichia and Candida. In the microflora of Cabernets, Risling and, especially, Karaburnu grapes, we have found lactic acid bacteria Lactobacillus plantarum and Lactobacillus brevis. Lactobacillus plantarum. Among the bacteria the total quantity of coccic flora composes - 56 %, the rod-shaped - 44 %. Two types of active acetous bacteria are identified – Acetobacter aceti and Acetobacter xelinum. Especially high was their surface concentration on the berries of Kachich type of grapes
This article discusses the actual problem of using SSR-markers in studies of the genus Prunus. We have examined the works on the most important cultures of genus, collected and analyzed contemporary literature of the subject. Data of the initial stage of Prunus SSR-based genetic diversity study in the North-Caucasian region has been described in the presented publication
This article presents the results of testing IRAP DNA
markers Cass1 and Cass2 applied to Prunus spinosa.
The findings suggest the high perspectiveness of
their using for the study of genetic diversity of the
gene pool of this species. According to the results of
the analysis of the sample 12 genotypes were
identified from 6 to 13 fragments in the spectrum of
Cass1 and from 5 to 11 fragments for Cass2. As a
result of cluster analysis in the sample formed three
groups of samples. In one of the groups, which is
most distant from the other two, includes samples
taken in Ukraine, while the remaining two groups
included samples from Armenia, the Krasnodar
region region, the Republic of Adygea, Ukraine and
Moldova, and three cultural large-fruited form. The
distribution of samples in clusters corresponded to
their geographical origin that favors the objective
assessment of genetic distances between the samples
using Cass1 and Cass2 markers. Thus, it was
concluded that the prospects of using DNA markers
to study the genetic diversity within a species of
Prunus spinosa
Estimation of self-incompatibility allelic diversity in Russian apple varieties was conducted. We have established S-allele gene set for a number of varieties. The most common allele of gene was identified. Alleles frequency data obtained are consistent with those in the world apple germplasm
Industrial horticulture assumes the most effective use of
the potential of varieties. One of the key factors
determining the yield of garden plantings is the
effectiveness of pollination. To obtain the maximum
yield, it is necessary to ensure maximum pollination
during the flowering period. For this reason, much
attention has been paid to the selection of pollinators.
Crab-apple forms are promising for use as pollinators, so
this work was aimed at identifying the most common
alleles of the self-incompatibility gene in the crab-forms
using the molecular genetic method of analysis. The
object of the study was 29 apple-tree creams and 3 elite
selection forms. They carried out the molecular genetic
identification of alleles S2 and S10, which are among
the most common apple trees in the world gene pool.
Allele S2 was identified in 16 samples (14 forms and 2
elite selection forms), while S10 allele in one
sample (elite form 12/2-20 (24-28)). Data on the allelic
composition of the S gene in the samples studied are of
value for the formation of a genetic passport on the
compatibility of the studied samples of apple with
modern industrial varieties
Estimation of blast resistance of rice breeding lines, carrying resistance genes Pi-b, Pi-z and Pi-40 has been done. For phytopatological test isolates of pathogen from Krasnodar territory were used. As the results of the study Pi-40 gene showed maximal resistance to blast isolates. On the other part, rice line, carrying both genes – Pib and Pi-z showed significantly higher level of resistance with compare to standart variety Khazar
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
Multiplex DNA-marker set for PCR identification for rice blast resistance genes Pi-40 and Pi-b was developed in this study. Optimal primers combinations and PCR conditions allows to identify both abovementioned genes in the single PCR
The results of development of rice breeding lines, carrying the wide range resistance gene to rice blast disease - Pi-40. For identification of the dominant allele of the gene the DNA - marker analysis was used. With co-dominant DNA markers plants from inbred populations that carry a dominant allele of this gene in the homozygous state were selected