Name
Zvyagin Andrey Sergeyevich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
Kuban State Agrarian University
Web site url
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Articles count: 15
Extraction of the DNA from leaves of genus Vitis is a very difficult task, as a grape has a lot of elements, which make difficult to use it in the genetic manipulation. In that article used the methods of extraction of the DNA with silica (SiO2) particles as the absorbent material for getting better quality DNA
Two methods were used for DNA extraction from herbarium leaves of two subspecies: V. vinifera subsp. sativa D.C. и Vitis vinifera ssp. silvestris Gmel. DNA extraction is difficult from herbarium leaves plants because of the presence of metabolites that interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning. The quality of DNA was checked on a spectrophotometer by measuring the level of absorption at A260/280 nm. The results showed the successful extraction from mature and dried leaves of DNA when using the СТАB-method №2. Another method hasn’t shown satisfactory results in amplification of DNA
The clone selection has very outstanding position behind the actual problems of modern viticulture in the world and in our country. This problem usually has the primary importance behind the scientist who works with grape in all the countries such as USA, German, French, Spain, Italy, Hungary, Bulgaria, Moldavia, Ukraine and etc. In our country last years some positions becomes solving in the plane of scientific research work. Consequently, in the article the history and the situation of clone selection of grape on the Eurasian continent is widely covered.
The polymorphism is the diversity of varieties in ampelografy - the presence simultaneous permanent in the population of two or several phenotypic and genotype differing groups of plants of one variety – i.e. clones. In viticulture the isogenic population is usually called “clone” and variety as the total of clones or forms – i.e. it is heterogenic. Polymorphism of varieties has big practical sense, as varieties-clones have the harmonic complementary adaptive ampelocenosis and higher economical effect for production. In the article the polymorphism of two heterogenic population - the group Pinot and Riesling, which are the best specimen of French and German viticulture is described on the phenotypic level of leaves