The article presents the first results of studies of
somaclonal variability, which take place during
cultivation of tea plants tissues and organs in vitro
culture. As a starting material, there was used
morphogenic callus, isolated from the basal part of tea
microshoots, because callus increases somaclonal
variability. An optimized protocol of the nutrient
medium for induction gemmogenesis to product somatic
clones of tea plants in vitro culture was developed.
Morphogenesis calluses, initiated from the basal part of
tea microshoots and subcultured on the nutrient medium
with 6 – BAP – 2,5 ml + gibberellic acid – 1,0 ml +
Tidiazuron – 4,0 ml + Tryptophan – 1000 mg,
distinguished by high rates of induction of
gemmogenesis – 63,3 %. The article studies the effect of
exogenous growth regulators on morphological and
growth indices of tea callus culture in vitro
The pathogenic agent of apple scab disease, Venturia
inaequalis (Cooke) Winter, causes significant damage
to the industrial production of apples. Studying the
pathogen in pure culture is important for solving the
theoretical and practical issues of its biology. The
sporulation ability of the Venturia inaequalis
laboratory culture is an important diagnostic feature in
morphological culture analysis, in the test for
sensitivity to fungicides, when creating a collection
inoculum for an artificial infection. We have studied
the morphological features of conidiogenesis of pure
V. inaequalis culture, such as the location of
conidiogenic structures and their form. For the first
time in the laboratory culture of the pathogen,
sporulation in the thickness of agar on the substrate
mycelium was demonstrated. Conidiogenesis
proceeded according to the blastic-annellidic type.
Depending on the location of the conidiogenic
structures on the aerial or substrate mycelium, their
morphology was different. Conidiogenic areas in the
substrate mycelium could be observed with the
unaided eye, in the form of hyphal grit, while they
were conidia conglomerations in each annelid.
Annellide had a curved shape. On the aerial mycelium,
annelids were straight and always had only one
conidium. Differences in the morphology of
conidiogenic structures are supposed to be related to
the physical conditions of the environment in which
sporulation takes place. In the agar, each mature
conidium remaining at the apex of the annelid
interferes with the formation of the next one, which
results in its bending. The fixed arrangement of
conidiogenic structures and forming conidia in the
thickness of agar allows the use of substrate
sporification for model studies of the conidiogenesis
process
The work was carried out in the scientific-research
laboratory of the chair of Biotechnology, Biochemistry
and Biophysics of Kuban SAU, the aim of which is the
search of optimal conditions of obtaining the
hydrolyzates of lactic acid bacteria by thermoacid and
enzymatic ways. The pure cultures of microflora of
gastrointestinal tract of quails − Lactobacillus agilis,
Lactobacillus intermedius and Lactobacillus salivarius
were the object of researches. Under conducting the
thermal acid hydrolysis there were used the same
correlations of bacterial mass and water as well as
procedures of clearing of hydrolyzates. The variable
conditions were рН, temperature and time of hydrolysis.
When carrying out the enzymatic hydrolysis in this
study there was used the enzyme from the group of
muramidase lysozyme. The amount of enzymes and
time of carried out hydrolysis were the variable
conditions under obtaining the enzymatic
hydrolyzates. In the process of researches there were
studied: qualitative content of total protein, peptides
with М.м. < 1500, protein and peptides with M.m. >
1500, aminoacids and as well as GMDP
(glucosaminilmuramilpentapeptide). On the basis of
carried out experiments there was stated that according
to all components of biologically active substances the
thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates
by different ways was revealed at destruction of cells
Lb. intermedius. Content of GMDP in thermal acid
hydrolyzate was higher than in enzymatic on
0,028 g/100 ml (51,8 %)
The study of agronomy plants history, its distribution
and use through image analysis and iconography have
been presented. As an object of study, the image of
wheat has been taken as the ancient culture of the
world among agricultural plants. After many years, the
illustrative series takes on a completely different
meaning, namely, it becomes an independent resource
of information. The analysis includes images of wheat
in painting, sculpture, mosaic, tapestries, murals,
botanical illustrations, stamps, porcelain and scientific
drawings. In the work, we have made an attempt to
analyze the images of wheat in the works of art in
order to search for species diversity, directions of
selection and genetic research, and also to present the
morphological characteristics of culture. The method
of analysis is a method of visual notes or sketches,
which consists in comparing and searching
information by image. The analysis is carried out on
grounds: tall wheat, multiflorous forms of wheat, nonspreading,
thickness of straw. The visual analysis of
the images of wheat in works of art by the method of
sketches made it possible to reveal the species
diversity of culture along the ear, to see the ancient
forms that were cultivated in the world before the
"green revolution". The analysis included works of art
from the 14th to the 21st century, the masters of
painting in Italy, Germany, Russia, the Netherlands,
France, the United States, and others
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
In the course of the work, 33 ISSR markers were
evaluated for efficacy in the detection of genetic changes
in regenerants of Galanthus woronowii Losinsk.. Ten
markers were found suitable for genotyping according to
the species under study. Five samples from the selected
ten were analyzed for a sample of 20 plants of
regenerants and a mother plant. The obtained data testify
to genetic stability of plant material in the process of
microclonal propagation
The article presents the structural and phylogenetic
analysis of VIT_02s0033g00410,
VIT_02s0033g00390 and VIT_02s0033g00450
genes of the grapevine genome and the closely
related orthologous gene MYB114 of the
Arabidopsis genome. These genes are responsible
for the biosynthesis of anthocyanin in the organs of
model plants and are of interest not only for practical
production and breeding, but also for fundamental
research. These genes were analyzed for GCcomposition
of nucleotides, the presence of cisregulatory
elements and promoter regions. DNA and
protein sequences were aligned to look for similar
elements, which allowed further analysis of the
ultraconservative domains of four genes. Based on
the results of search and identification of the
conservative regions, a cluster tree was constructed,
which made it possible to identify the separation of
gene sidelines from, presumably, the main one. At
the same time, the construction of consensus trees
based on DNA and protein sequences revealed their
absolute similarity. "The Minimal Evolution Tree"
allowed calculating the approximate dates of the
appearance of the mutations and the divergence
times of the gene branches between each other. At
the same time, the appearance of the Vitis genus and
its separation from the Rosales was taken as the time
first divergence point. In the end, homologous
metabolic pathways were searched between
grapevine and Arabidopsis, which revealed the
presence of homologous proteins in the grape
proteome. In this turn, it already confirms the
existence of similar biosynthetic pathways and, as a
consequence, interactions such as "DNA-protein"
and "protein-protein"
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The analysis of the accumulation and distribution of
heavy metals in the stevia raw material grown in
different agroecological regions is carried out. The
elements present in the soils always have a complex
effect on the plant and at the same time enhance or
weaken each other's action. Plants of stevia are able to
regulate the flow of the heavy metals under study,
assimilating organs accumulate the optimal number of
elements necessary for their growth and development,
regardless of the growing conditions
This article presents the results of testing IRAP DNA
markers Cass1 and Cass2 applied to Prunus spinosa.
The findings suggest the high perspectiveness of
their using for the study of genetic diversity of the
gene pool of this species. According to the results of
the analysis of the sample 12 genotypes were
identified from 6 to 13 fragments in the spectrum of
Cass1 and from 5 to 11 fragments for Cass2. As a
result of cluster analysis in the sample formed three
groups of samples. In one of the groups, which is
most distant from the other two, includes samples
taken in Ukraine, while the remaining two groups
included samples from Armenia, the Krasnodar
region region, the Republic of Adygea, Ukraine and
Moldova, and three cultural large-fruited form. The
distribution of samples in clusters corresponded to
their geographical origin that favors the objective
assessment of genetic distances between the samples
using Cass1 and Cass2 markers. Thus, it was
concluded that the prospects of using DNA markers
to study the genetic diversity within a species of
Prunus spinosa