Scientific Journal of KubSAU

This article presents the possibilities of using organic fertilizers, which allow you to recharge grape raw material with biologically active substances and improve the nutritional value of wine production. The influence of organic fertilizers on activation of process for detoxification of soil toxic compounds has been measured, ensuring food safety of raw materials for the production of grape wine products by reducing of hazardous chemicals. The use of biotechnology preserves and also enhances the uniqueness and diversity of the chemical composition of the grape raw material. We have established that after the four-year application of biotechnology the content of toxic compounds in grape raw material was reduced by 7%, and the concentration of biologically active substances increased in 1,5-2% times in comparison with the production of commercial plantations

The article describes the history of the institute called "Magarach" and the formation of the world's ampelographic collections in the Crimea and Kuban, provides data on the genetic diversity of the original parent Crimean collection of grape, carries out the impact of its long-term ampelo-breeding and genetic work. We have found the best ampelo-genetic rennets, both in the Crimea (zoned in Russia and abroad varieties of Academician Avidzba, Pervanets Magaracha, Jubilee Magarach, Antei magarachsky, Nimrang ustoichivii, Podarok Magaracha, Riesling Magaracha Novoukrainsky Rannii, Granatovii Magaracha, Krymchanin, Rkatsiteli Magaracha, Spartanets Magaracha, Tavkveri Magaracha, Citronnii Magaracha, promising varieties Livya, Solnetchnaja grozd, etc..) and in the Kuban region. Magarach ampelographic collection was and still is the parent of many national collections of gene pools of, the former Soviet republics, including the Russian Federation as well: Anapa ampelographic collection in the North Caucasus NIISiV has 3356 samples collected from 41 countries, including ones from the institute of "Magarach" (about 40%). Using its base we had a comprehensive biological and economic evaluation of the outstanding genotypes for ampelo-genetic and clonal selection – such as zoned Bogotyanovsky, Helios, Dolgogdannii, Kubattik, Libya K, Arathi, Preobragenie, Rochefort K, Chrizolit, Citrine, Anthracite, promising varieties Annie, Baikonur, Gourmet Kra, Memory Master Nesvet Gift, Jubilee Novocherkasska, etc.

The article presents a study on agro-biological and technological indicators of Don indigenous wine grapes in the conditions of the Anapa-Taman area of the viticulture of the Krasnodar region. It was found that the introduced varieties are consistent with the conditions of the area and can be recommended for growing in production plantations in southern Russia. On ripening, the varieties are as follows: the early ripening varieties are Sibirkovy; a group of middle-ripening - Aligote, Plechistik, Varyushkin; to the medium-late - Bessergenevsky 10 and Krasnostop Anapsky. Highest yield was proved to for such grape varieties as Bessergenevsky 10 (150 kg / ha) and Varyushkin (140 kg / ha). Test grapes of Aligoté and Krasnostop Anapsky showed an average yield of 120 and 130 kg / ha, respectively, red and Sibirkovy Plechistik - 140 and 135 kg / ha. Comparing the current literature with the results, we noted the following notable differences: all the studied indigenous wine grapes in the conditions of the Anapa-Taman area of the Krasnodar region matured earlier, had higher productivity indexes and gave higher yields, than in the conditions of their homeland Rostov region

The article discusses the use of automatic systemic-cognitive analysis (ASC-analysis), its mathematical model is a system of information theory and software tools – an intellectual system called "Eidos" for the solution of some problems of ampelography: 1) digitization of scanned images of the leaves and creation of their mathematical models; 2) the formation of mathematical models of specific leaves using the spreading of information theory; 3) the formation of models of generalized images of leaves of various sorts; 4) comparing an image of a specific leaf with a generalized image of the leaf of different varieties and finding a quantitative degree of similarity and differences between them, i.e. the identification of the varieties on the leaf; 5) quantification of the similarities and differences of the varieties, i.e. cluster-constructive analysis of generalized images of the leaves of different varieties. We propose a new approach to digitizing images of leaves, based on using the polar coordinate system, the center of gravity of the image and its external contour. Before scanning images we may use transformation to standardize the position of the still images, their sizes and rotation angle. Therefore, the results of digitization and ASC-analysis of the images might be invariant (independent) relatively to their position, size and rotation. The specific shape of the contour of the leaf is regarded as noise information on the variety, including information about the true shape of the leaf of the class (clean signal) and noise, which distort this true form, originating in a random environment. Software tools of ASC-analysis – intellectual "Eidos" system ensures noise reduction and the selection of the signal about the true shape of the leaf of each variety on the basis of a number of noisy concrete examples of the leaves of this variety. This creates a one way form of a leaf of each class, free from their concrete implementations, i.e., the "Eidos" of these images (in the sense of Plato) is a prototype or archetype (in the Jungian sense) of the images

The article presents the results of the studies of 11 varieties cultivated in the eastern region of South Coast wine-growing zone of Crimea (planting of "Solnechnaja Dolina ", near Sudak mountain) in the area of their natural growing, where was formed the gene pool of grapes for a long time. As a result, the three-year (2010-2012) study found that the majority of indigenous varieties had small number of fruitbearing shoots in the bush: the varieties with colored berry - 53,3-73,3%, in white-berry - 46,6-75,9% except Shabash variety - 79.9%. We have identified the most productive varieties which were Dzhevat kara (62.2 t / ha), Kokur Beliy (48.9 t / ha), Kapselsky Beliy (44.4 t / ha), and varieties with female type of flower: Kefesiya, Ekim kara, Kock pandas, Sary pandas and Crona gave not high yield: 22.2; 22.2; 24.4; 28.9 and 28.9 t / ha. On the strength of growth, productivity and the accumulation of sugars in the juice of berries, we picked out varieties of Dzhevat kara, Crona, Kapselsky beliy. According to the main economic indicators, it turned out that the most profitable varieties are Dzhevat Kara, Kokur beliy and Kapselsky beliy. Hence, these varieties in conditions of the Crimea have much greater prospect of reproduction and development of production

The article presents historical information about the stages of gradual transformation of the first non-large wine-making institution of Crimea, organized in 1828 under the initiative of Prince M.S. Vorontsov in the natural boundary called Magarach near the Imperial Nikitsky botanical garden in the world famous Institute of Grape and Wine "Magarach" - once the chief coordinator of the research work in the field of viticulture and winemaking republics of the USSR and the main forge of scientific staff in this direction. Among the directors of the institute of the last century, there were such talented organizers as A.G. Globa, T.G. Kataryan, P.Y. Golodriga. The Institute operated a network of regional branches, created enotec with a settled in 1836 wine Muscat pink Magarach (World rarity is reflected in the Guinness book of records), the world ampelographic collection (fourth in number of samples), scientists developed area-standard assortment for production, issued 11 Volumes of "Ampelography of USSR" (awarded by OIV), designed a lot of outstanding for yield and stability varieties (Pervenets Magaracha, Ranniy Magaracha, Citron Magaracha et al.) and clones (Muscat white of Red Stone, Pinot Noir yields and others.) grapes. There was a significant contribution in the sphere of research and development of viticulture and winemaking of world, the institute was given an international prestige and this led to establishing the base of the General Assembly of the OIV - International Organization of Grape and Wine, the International Symposium on the selection of wine-town, several international wine tasting competitions and International finest examples of table grapes

Progress of breeding for resistance to diseases, pests and low temperatures is provided by cross-species hybridization. Institute of Vine and Wine "Magarach" is one of the leading centers of selection of grapes in the world. The breeding program of deducing grapes at the institute is based on the study and attracting the world's gene pool, including and industrial of the Russian Federation assortment of grapes. Analysis assortment shows that the share of productive, highquality varieties with group resistance to diseases, pests and extreme environmental factors is very small. The main method of selection of grapes of a new generation who have genetically determined traits earliness, resistance to biotic and abiotic factors is crossing complex interspecific hybrids and forms of their involvement with each other and with the forms of species Vitis vinifera, belonging to different ecogeographical groups, saturating crossing between hybrid forms with complex features, the appropriate selection of reference. For vigorous offspring is most effective as a parent to use a local variety of forms of Crohn and father - interspecific cultivar Alminsky

Peculiarities of breeding oidium-resistant genotypes in crossing Crimea autochthonous grape varieties with complex interspecific hybrids were studied. Twentytwo populations consisting of 1323 seedlings were analyzed over a period of 2008-2014. The highest oidium resistance was registered in the progeny of the cross Khersonesskii х ЖС 26-205 (6.8 points), and the average estimate of oidium resistance across all the study populations was higher than that of the initial autochthonous grapes of the Crimea. The level of hypothetical heterosis in the majority of the study populations indicates that, on the whole, the hybrid seedlings possess a higher oidium resistance relative to the initial Crimea autochthonous grapes. The highest breeding value was associated with cross combinations having the complex interspecific hybrids Muscat JIM, Spartanets Magaracha and Magarach 31-77-10 in their pedigrees. The cross combination Muscat JIM х Shabash was the most efficient, yielding 10.7 per cent of oidium-resistant seedlings. It was reliably established that a high level of the genotypically determined inheritance of oidium resistance is found in crosses with the participation of the interspecific forms Magarach № 31-77-10 and Muscat JIM (female parents) and Spartanets Magaracha and Tsitronnyi Magaracha (male parents). The expediency of this direction of breeding research was proved

This article presents information about the features of biotechnology as the driving force of scientific and technological progress. The national programs of the leading countries of the world, it is one of the priority sectors, reflecting the level of the socio-economic condition of the society. Biotechnology is now successfully solves such vital tasks as providing food, the establishment of effective medicaments, obtaining fuel based on renewable raw materials, maintaining ecological balance, conservation of biological resources of the Earth. The development of agriculture in modern conditions is impossible without agricultural biotechnology. It is directly related to viticulture. Choosing an object of an integrated system (embryos, apical meristem, axillary buds), it is possible to clone plants, i.e. produce plants identical to the original. If the same as the object to use isolated cells or protoplasts, in this case, there will most likely altered versions, creating diversity for the breeder. Genetic engineering – the science of younger, since the establishment of the first chimeric DNA molecule. The origin of genetic engineering is rooted in the development of molecular genetics, biochemistry. These technologies, undoubtedly progressive, but their biological safety is still insufficiently explored and is a danger to all life on Earth. The leading Western powers carried out strict control over the introduction of transgenic crop plants, as they are in agrocenosis new biological risks that may adversely affect the plants, animals and humans. In Russia, as in other countries, have already adopted the law “State regulation of genetic engineering”

The article discusses the application of automated system-cognitive analysis (ASC-analysis), its mathematical model is a system of information theory and implements, its software tools – intellectual system called "Eidos" for solving one of the important tasks of ampelography: to quantify the similarities and differences of different clones of grapes using contours of the leaves. To solve this task we perform the following steps: 1) digitization of scanned images of the leaves and creation their mathematical models; 2) formation mathematical models of specific leaves with the application of information theory; 3) modeling the generalized images of leaves of different clones on the basis of specific leaves (multiparameter typing); 4) verification of the model by identifying specific leaf images with generic clones, i.e., classes (system identification); 5) quantification of the similarities and differences of the clones, i.e. cluster-constructive analysis of generalized images of leaves of various clones. The specific shape of the contour of the leaf is regarded as noise information on the clone to which it relates, including information about the true shape of a leaf of this clone (clean signal) and noise, which distort the real shape, due to the random influence of the environment. Software tools of ASA-analysis which is intellectual "Eidos" system provides the noise suppression and the detection of a signal about the true shape of a leaf of each clone on the basis of a number of noisy concrete examples of the leaves of this clone. This creates a single image of the shape of the leaf of each clone, independent of their specific implementations, i.e. "Eidos" of these images (in the sense of Plato) - the prototype or archetype (in the Jungian sense) of the images