The research shows the possibility of extended
preimplantation genetic screening (PGD) that allows
to improve the strategy of selection of embryos that
satisfies not only the requirement of the absence of
chromosomal abnormalities, but also includes their
additional check for predisposition to various diseases,
as well as the choice of the embryo with the most
optimal HLA haplotype in cases with expressed
compatibility of parents for HLA-genes
The results of the questionnaire survey of cadets of
Maritime University, Maritime College and students
who had been given training in the Institution of
Advanced Training are presented. It is revealed that
the level of motivation of ship personnel for first aid
treatment to victims on commercial ships is two times
lower than that of cadets. The conclusion about the
necessity of solution the legal aspects of sailorsparamedics,
working under the Russian flag was made
The retrospective study of patients with retinal vein
occlusion and frequency of pseudoexfoliation
syndrome is made in this study. Retinal vein occlusion
is the second most common type of retinal vascular
disorder, after diabetic retinal disease, and may
develop with a consequent reduction in visual acuity.
Pseudoexfoliation syndrome is an age-related disease
characterized by the production and accumulation of a
fibrillar extracellular material in several ocular tissues
We have analyzed data of 73 patients with retinal vein
occlusion. It was detected that risk of central vein
occlusion is higher in patients with pseudoexfoliation
syndrome (23,3% of patients were diagnosed as having
pseudoexfoliation syndrome). In the branch retinal
vein occlusion group only 9,3% of patients had
pseudoexfoliation syndrome. In control group only
8,6% of patients had this syndrome. Control group was
made of 80 patients similar in age without any retinal
and cardiovascular abnormalities, patients with
episodes of ocular hypertension, glaucoma, and those
who had undergone cataract surgery were excluded
from the study. Pseudoexfoliation syndrome may be
considered as an independent risk factor for
development of central retinal vein occlusion
It is known that there are most widespread the
diseases of cardiovascular system among the
examined seamen. We have carried out the analysis
of distribution of concrete nosological forms of
pathology of cardiovascular system and concomitant
diseases (gastrointestinal tract, nervous system, ENT
organs, the musculoskeletal apparatus and others).
Results of preventive screening of the crew
personnel performed in 2007–2010 by medical
commission of Novorossiysk clinical center of
Russia’s Federal Medico-Biological Agency were
subject to scientific research. 3352 seamen aged
from 18 to 60 years (middle age 27,2+1,3 years),
among which 2627 surveyed – personnel of bluewater
shipping, 725 surveyed – personnel of
domestic shipping have been subjected to studying.
The examined contingent is ranged taking into
account professional groups. It is revealed that
cardiovascular pathology is almost equally
widespread in all professional groups of personnel of
ships of domestic and blue-water shipping. This
pathology occurs at 13,5–21% of the seamen
corresponding who had medical examination. The
main group of the examined seamen (79–96,7%)
belongs to the category «almost healthy» and
suitable for work in the sea. Among revealed
pathology at seamen, the high specific weight (63–
70%) belongs to diseases of cardiovascular system. Probable intensive work and harmful factors of the
ship environment in each of professional groups
exert significant negative impact on psychic and
somatic health of seamen. Neurocirculator dystonia
(hypertensive and cardio types) dominates in
structure of cardiovascular pathology registered at
seamen of both professional groups. The associated
diseases (71–82%) meet more often at persons with
hypertension, manifesting forms of atherosclerosis
and ischemic disease. In both groups, we have noted
the high percent of violations of digestive tract,
musculoskeletal apparatus, alimentary and
constitutional obesity and eyesight. However,
alimentary and constitutional obesity and diseases of
musculoskeletal apparatus are most characteristic of
the personnel of ships performing long maritime
traffic. Obviously, this phenomenon is connected
with hypodynamia
While studying the content of hypoxia-inducible factor
1-alpha and vascular endothelial growth factor in the
tumor tissue, oral fluid and peripheral blood of 141
patients with oral mucosa cancer T1-3N0-2M0, it was
shown that the development of purulent inflammatory
postoperative complications in patients with oral
mucosa cancer is associated with an increased
expression of HIF-1α in the tissue and its translocation
from the cytoplasm to the nucleus, activation of the
neoangiogenesis processes. It is informative to
determine HIF-1α factor in the blood serum, which,
for further development, can be used as a non-invasive
diagnostic marker to predict the postoperative course
in this category of patients
The problem of rendering of first medical aid to
personnel of commercial ships under the Russian flag
is considered. It is shown that the decision of this
problem in our country is impossible without juridical
aspects of activity of sailors-paramedics, questions of
their training in bases of rendering an emergency aid
and due medical support of the Russian ships. The
emergency elementary aid has to be an integral part of
activity of personnel of ships. Some directions of the
decision of a problem of efficiency of providing such
help to personnel of ships are proposed. Development
of the bill directed to introduction in Russia of
requirements for assistance by paramedics on
commercial ships is necessary
The article presents an analysis of features of the
organization of special medical and prophylactic
support on commercial ships. First-aid treatment at
poisoning of crews of the most widespread toxic
matter (poisonous gases, oil products, methane,
coolants, and fumigants) has been considered. It is
noted that medical aspects of toxic dangers on the
water transport have to include prevention of the
pathological states of health of seamen, timely medical
support of the ship, early diagnostics of pathological
states, sorting of injured persons and the organization
of first emergency medical aid
A retrospective immunohistochemical study of lymphocyte and macrophage infiltration in tumor tissue of 84 vulvar cancer patients with a long (over 2 years) and short (less than 2 years) remission was performed to determine a possible role of immunologic microenvironment of tumors in maintaining remission. Vulvar cancer patients with a short remission period were aged 46-85 years, mean age 67 years. 15.8% of them had stage I of the disease, 21% - stage II, 47.4% - stage III and 15.8% - stage IV. The average duration of remission in this group was 11 months. Patients with a long remission period were aged 27-71 years, mean age 48 years. 38.5% of them had stage I of the disease, 53.8% - stage II and 7.7% - stage III. The average duration of remission in this group was 8 years. Histological study showed squamous cell carcinoma in all patients. Stroma and parenchyma of tumor tissues demonstrated lymphocyte and macrophage infiltration of various degrees with the predominance of T-lymphocytes. CD8+ cells prevailed in tumor tissue of patients with a long remission period (over 2 years), while a higher level of CD4+ Т-lymphocytic infiltration was associated with a short remission (less than 2 years) and a poor prognosis. These differences allow prediction of the vulvar cancer course and its early or late recurrence
The aim of the study is to compare the traditional
clinicopathologic factors and prognosis of cell cycle
parameters, such as ploidy, cell cycle kinetics, as well
as the study of DNA content in the tumor tissue of
patients with esophageal cancer. Cancer of the
esophagus - a very aggressive malignant disease, most
commonly manifested impaired swallowing. As a rule,
this type of cancer pathology is characterized by
unfavorable outcome. Ploidy and cell proliferation
activity, defined by the content of the DNA of the
tumor - synthesizing cells are one of the important
indicators of the growth rate of malignancy and
prognosis. The study was performed on tumor tissue
fragments of the 30 patients operated on for cancer of
the esophagus. DNA study - conducted in the open cell
material aftercare. DNA cell content was determined
by flow cytometry BD FACSCanto II. By studying the
DNA-cytometric parameters, we found the prevalence of aneuploid tumors in 66.7%. It was shown that DNAcytometric
parameters are independent of gender.
Major weight of diploid and aneuploid cell tumors of
the esophagus was in G0 / 1 phase of the cell cycle.
Comparative analysis of DNA - cytometric parameters
and the degree of differentiation of esophageal tumor
revealed a prevalence of aneuploid tumors in all the
test cases. content distribution pattern of tumor cells
was found in the G0 / G1-, G2 + M -, S - phase of the
cell cycle at different stages of the disease
Isolation of fetal cells from the blood of a pregnant
woman is more relevant for receiving the information
of the genome of a fetus and implementation in clinical
practice, non-invasive screening methods for prenatal
diagnosis. The work proposes efficient ways of sorting
trophoblasts circulating in maternal blood for the
diagnosis of fetal aneuploidy in early gestation by
analyzing microsatellite marker of the chromosomes
that most prone to numerical aberrations (13, 18, 21, X
and Y). A set of microsatellite markers allowed to
reveal aneuploidy in three pregnant women, and also
to exclude mosaicism and contamination of samples
with maternal cells. A retrospective analysis of data on
invasive cytogenetic studies (chorionic villus
sampling) confirmed the results of the study