Name
Prudnikov Sergey Mikhailovich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Krasnodar Research Institute of Agricultural Products Storage and Processing
Web site url
—
Articles count: 3
To determine the mass fraction of linolenic acid in flax
seed oil the authors developed a rapid method based on
the pulse method of nuclear magnetic resonance with
the use of NMR analyzers low resolution. This method
has some significant advantages compared with the
known method on the basis of chromatographic
method for the determination of the mass fraction of
linolenic acid in the oil extracted from the seeds of
flax, namely, does not require additional sample
preparation (extracting oil by solvent), eliminates the
use of toxic chemicals, requires a much lower
investment of time to conduct a single analysis, highly
automated, and eliminates the influence of human
factor on the research results. The method is based on
the revealed correlation between the weighted average
time spin-spin relaxation of protons contained in the
triacylglycerols of the oil of the flax seed, and a mass
fraction of linolenic acid in the seed oil at a
temperature of 23ºc. In article results of research of
influence of temperature on the weighted average time
spin-spin relaxation of protons contained in the flax
seed oil. Installed directly proportional linear
relationship between the temperature of flax seeds and
measured the average time spin-spin relaxation of
protons oil contained in the flax seeds with the correlation coefficient is 0,930, the temperature change
of the sample of seeds at 1 ° C leads to the change of
the coefficient is 0.011 kt. The developed
methodaccuracy in the temperature range from 20 to
26 ºC
In recent decades, Russia has increased the demand for
high oleic sunflower oil and, consequently, for the raw
material from which it is derived – high oleic sunflower
seeds. Currently existing methods of control of mass
fraction of oleic acid in the seed oil of sunflower, have
significant drawbacks, due to which sharply raises the
question of the development of the Express methods of
control of mass fraction of oleic acid. The study presents
the results of determining the mass fraction of oleic acid
in the oil of sunflower seeds, obtained by known
methods (GC and ri), and developed a method based on
NMR. The developed method of determining the mass
fraction of oleic acid in sunflower seed oil has a number
of advantages compared with the known: is not
destructive (does not require the extraction of oil from
seeds), does not require special sample preparation
excludes the use of toxic chemicals, the analysis duration is 5 minutes, ensuring high representativeness of the
sample, and the simplicity and automation of the analysis
process reduces the requirements for qualifications of
laboratory personnel. The developed method of
determining the mass fraction of oleic acid in the oil of
sunflower seeds can be carried out simultaneously with
the determination of oil and moisture content of seeds on
commercially available analyzers, but I hope it 1006М,
which is currently applied at more than 250 companies of
oil and fat industry
The article presents data, on the basis of which we
have concluded that the oil and phospholipids
contained in soy lecithins, the degree of unsaturation
of fatty acids differ significantly from oil and
phospholipids contained in sunflower lecithins, with
differences in the degree of unsaturation of fatty acids
of oil and phospholipids contained in sunflower and
soy lecithins, leads to differences in the content of
resonating protons, and, consequently, the level of
amplitude values of NMR signals of protons of the
investigated lecithins. It is established that the values
of the amplitudes of the NMR signals of protons of
each of the four components of sunflower lecithins
differ from the values of the amplitudes of the NMR
signals of protons the component of soy lecithins,
which confirms the differences in the composition and
content of fatty acids lecithins studied. Considering
quantitative characteristic of mass fraction of
substances insoluble in acetone, i.e. phospholipids, is
the sum of the amplitudes of the NMR signals of
protons of the third (A3) and fourth (A4) component,
was built the graphical dependence of the mass
fraction of substances insoluble in acetone, from the
value of (A3+A4)/ASIS, % which is described by the equation (correlation coefficient R2
=0,9911) of the
following form: y=0,8177x +2.52. Based on these
data, we have developed a rapid method of
determining the mass fraction of substances insoluble
in acetone, soy lecithin, eliminating the use of toxic
organic solvents, is environmentally safe, which is
protected by RF patent for the invention