Name
Gvozdetskii Nicholai Alekseevich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
Stavropol State Agrarian University
Web site url
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Articles count: 1
The article presents the use of intensive methods of
diet herd reproduction that will increase the
productivity and profitability of the sheep industry.
One of such methods is in vitro fertilization (IVF). For
the success of in vitro fertilization procedure, an
important requirement is the availability of highquality
nutrient media, which help to preserve the
genetic material and contribute to the further
development of the zygote. The main requirement to
the media for sperm is the ability of media not to cause
their agglutination. The aim of our work was to search
for new ways to reduce the agglutination of
spermatozoa in the preparation of freshly prepared
sperm in the process of production of embryos in vitro.
To eliminate the agglutination of spermatozoa in the
semen preparation stage, we used GCY medium,
followed by demolition of seed in SOFw environment,
which has resulted in a significant (almost 15 times!)
reduce of the number of bound sperm. In our opinion,
a decrease in agglutination in GCY buffer was
connected with a specific influence of the constituent
components of sperm.
Conclusion: Thus, our method of preparation of
freshly prepared sperm for in vitro fertilization allows
a sharp decline in sperm agglutination, which will
improve the fertility of eggs during the production of
embryos in vitro sheep