Name
Sibityatkin Sergey Vasilievich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
NPF “Sady Chechni”
Web site url
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Articles count: 1
The article presents the results of the study of the
effect of the size of the explant clonal rootstocks for
stone fruit crops of the Krymskaya OSS VIR VSL-1,
VSL-2, LC-52, RVL-1, RVL-7, VVA-1, AP-1 injected
in vitro and concentration vitamins and
phytohormones in a nutrient medium on the efficiency
of introducing in vitro of these rootstocks. The optimal
size of the injected in vitro explant was determined and
nutrient medium in the first stage of micropropagation
was optimized. The optimal size of the dissected
meristem, which provides the maximum percentage of
explant initiation is 0.4 mm. The modified version of
the nutrient medium based on the Murashige and Skug
prescriptions with a reduced content of vitamins B1 0.1
mg / l, B6 0.4 mg / l, PP- 0.4 mg / l, and phytohormone
6-BAP - 0.3 mg / l. at the first stage of cultivation of
explants of clonal rootstocks for stone fruit crops is
optimal. The survival rate of explants in all tested
samples of clonal rootstocks on this modified nutrient
medium was in the range of 92.5-97.5%