Name
Malyarovskaya Valentina Ivanovna
Scholastic degree
•
Academic rank
—
Honorary rank
—
Organization, job position
SSI "Institute of Floriculture and Subtropical Crops"
Web site url
—
—
Articles count: 4
It is shown that under in vitro conditions, regenerated plants of Lilium caucasicum cultivated in the light of varying intensity and spectral composition didn’t have the same biometric parameters (length and width of leaves) or number of micro bulbs. There were established some specific effects of the two main areas
of photosynthetically active radiation - blue and red on the growth and development of the plants as well as on the content of photosynthetic pigments
There were developed some methods of sterilization and introduction of the following endangered species of Caucasian flora to in vitro culture: Campanula sclerophylla Kolak., Lilium caucasicum Miscz. ex Grossh., Daphne woronowii Kolak., Pancratium maritimum L.. The effect of different plant hormones as well as their combinations and concentrations on the regeneration, growth and development of plants is studied. As a result of clonal propagation, more than 100 plants of Campanula sclerophylla Kolak, about 30 plants of Pancratium maritimum L., and 150 samples of Lilium caucasicum Miscz. Ex Grossh are saved in tissue culture
The study was aimed on approbation of 11 ISSR DNA-markers for their using in DNA fingerprinting of rare plant species of the Western Caucasus Lilium Caucasicum Miscz. Ex Grossh., Galánthus woronowii Kolak., Pancratium maritimum L. according to results DNA-markers set was selected for further using in the investigation of genetic diversity of the mentioned plant species
In the course of the work, 33 ISSR markers were
evaluated for efficacy in the detection of genetic changes
in regenerants of Galanthus woronowii Losinsk.. Ten
markers were found suitable for genotyping according to
the species under study. Five samples from the selected
ten were analyzed for a sample of 20 plants of
regenerants and a mother plant. The obtained data testify
to genetic stability of plant material in the process of
microclonal propagation