Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
AGRIS logo UlrichsWeb logo DOAJ logo

Name

Stepanov Ilya Vladimirovich

Scholastic degree

Academic rank

Honorary rank

Organization, job position

State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
   

Web site url

Email


Articles count: 9

Sort by: Date Title Views
749 kb

APPROBATION OF ISSR DNA-MARKERS FOR GENOTYPING OF GALÁNTHUS WORONOWII LOSINSK.. AND ANALYSIS OF GENETIC STABILITY OF PLANTS, OBTAINED BY IN VITRO CULTURE

abstract 1331709088 issue 133 pp. 1166 – 1178 30.11.2017 ru 189
In the course of the work, 33 ISSR markers were evaluated for efficacy in the detection of genetic changes in regenerants of Galanthus woronowii Losinsk.. Ten markers were found suitable for genotyping according to the species under study. Five samples from the selected ten were analyzed for a sample of 20 plants of regenerants and a mother plant. The obtained data testify to genetic stability of plant material in the process of microclonal propagation
1588 kb

APPROBATION OF MICROSATELLITE DNA-MARKERS FOR IDENTIFICATION OF APPLE CLONES

abstract 0841210021 issue 84 pp. 262 – 271 28.12.2012 ru 1176
As the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
383 kb

APPROBATION OF SSR MARKERS DEVELOPED FOR THE SPECIES OF PRUNUS PERSICA, IN RELATION TO DISCHARGE PLUM

abstract 1241610098 issue 124 pp. 1479 – 1490 30.12.2016 ru 276
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
259 kb

APPROVAL OF IRAP MARKERS BASED ON CASSANDRA RETROTRANSPOSON FOR THE ANALYSIS OF GENETIC POLYMORPHISMS IN THE GENUS PRUNUS

abstract 1041410055 issue 104 pp. 733 – 743 30.12.2014 ru 641
This study was aimed on testing of IRAP markers developed at plum on varieties of peach, Russian plum and cherry plum and genotyping of home and foreign varieties of plum, followed by an analysis of the data. During the testing of the markers have been identified as a high level of polymorphism between genotypes of plum and between the studied species. On the basis of the results obtained by genotyping 15 samples of Prunus was built dendrogram. Cluster analysis was divided into 3 groups corresponding varieties of P. persica, P. domestica and a common group for varieties of Russian plum and cherry plum
200 kb

ARTIFICIAL SCAB RESISTANCE EVALUATION OF MALUS ORIETALIS FORMS – A POTENTIAL SOURCE OF NEW GENES FOR RESISTANCE TO APPLE SCAB

abstract 1311707113 issue 131 pp. 1377 – 1388 29.09.2017 ru 92
Professional apple gardening is bound to particular risks, of which is essential losses of a harvest because of diseases. An apple scab, the caused Venturia inaequalis (Cooke) G. Winter, brings the greatest loss. The main approach in monitoring of a scab of an apple-tree is creation of grades, steady against pathogen. In the present work we have carried out phytopathology testing of generations of the seedlings received from the free pollination of six forms of a crab of Malus orientalis from a collection MOS VIR (Maykop) characterized by a relative resistance to the apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both of natural population of a scab, and of strains of pathogen of various cultivars and geographical origin. When carrying out padding infection increase in force of an infectious background that can be bound to selection of the plants of biotypes of a fungus that are most adapted for genotypes was noted. It is recommended for precise elimination of unstable plants at selection at early stages of an ontogenesis to carry out more than one serial infection during the season. By results of the phytopathological testing, we highlighted three Malus orientalis forms from the six studied No. 17982, 17985 and 3080 the most perspective stability genes for an introgressiya in a cultural gene pool of an apple tree
740 kb

IDENTIFICATION OF S2 AND S3 ALLELES OF SELF-INCOMPATIBILITY GENE IN CRAB APPLE AND ADVANCED BREEDING SELECTIONS

abstract 1321708084 issue 132 pp. 1076 – 1085 31.10.2017 ru 107
Industrial horticulture assumes the most effective use of the potential of varieties. One of the key factors determining the yield of garden plantings is the effectiveness of pollination. To obtain the maximum yield, it is necessary to ensure maximum pollination during the flowering period. For this reason, much attention has been paid to the selection of pollinators. Crab-apple forms are promising for use as pollinators, so this work was aimed at identifying the most common alleles of the self-incompatibility gene in the crab-forms using the molecular genetic method of analysis. The object of the study was 29 apple-tree creams and 3 elite selection forms. They carried out the molecular genetic identification of alleles S2 and S10, which are among the most common apple trees in the world gene pool. Allele S2 was identified in 16 samples (14 forms and 2 elite selection forms), while S10 allele in one sample (elite form 12/2-20 (24-28)). Data on the allelic composition of the S gene in the samples studied are of value for the formation of a genetic passport on the compatibility of the studied samples of apple with modern industrial varieties
246 kb

MOLECULAR-GENETICS BASE OF APPLE SELF-INCOMPATIBILITY

abstract 0801206025 issue 80 pp. 318 – 327 30.06.2012 ru 1162
Estimation of self-incompatibility allelic diversity in Russian apple varieties was conducted. We have established S-allele gene set for a number of varieties. The most common allele of gene was identified. Alleles frequency data obtained are consistent with those in the world apple germplasm
296 kb

USING OF RETROTRANSPOSONE CASSANDRA BASED DNA-MARKERS FOR EVALUATION OF GENETIC DIVERSITY OF PRUNUS SPINOSA SPECIES

abstract 1341710094 issue 134 pp. 1166 – 1176 29.12.2017 ru 20
This article presents the results of testing IRAP DNA markers Cass1 and Cass2 applied to Prunus spinosa. The findings suggest the high perspectiveness of their using for the study of genetic diversity of the gene pool of this species. According to the results of the analysis of the sample 12 genotypes were identified from 6 to 13 fragments in the spectrum of Cass1 and from 5 to 11 fragments for Cass2. As a result of cluster analysis in the sample formed three groups of samples. In one of the groups, which is most distant from the other two, includes samples taken in Ukraine, while the remaining two groups included samples from Armenia, the Krasnodar region region, the Republic of Adygea, Ukraine and Moldova, and three cultural large-fruited form. The distribution of samples in clusters corresponded to their geographical origin that favors the objective assessment of genetic distances between the samples using Cass1 and Cass2 markers. Thus, it was concluded that the prospects of using DNA markers to study the genetic diversity within a species of Prunus spinosa
153 kb

USING OF SSR MARKERS IN GENETIC STUDIES OF PRUNUS GENUS

abstract 0901306017 issue 90 pp. 294 – 307 30.06.2013 ru 1112
This article discusses the actual problem of using SSR-markers in studies of the genus Prunus. We have examined the works on the most important cultures of genus, collected and analyzed contemporary literature of the subject. Data of the initial stage of Prunus SSR-based genetic diversity study in the North-Caucasian region has been described in the presented publication
.