Name
Salfetnikov Anatoliy Alekseevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
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Articles count: 17
The article presents experimental data on the research
of gold nanoparticles (GNPs) with mean diameter 15
nm and 50 nm effect on seeds germination and growth
of 3-day-old etiolated coleoptiles and roots. Compared
with distilled water (control) initial colloidal solution
of 15 nm and 50 nm GNPs (57 µg/ml) had no
significant impact on seeds germination and growth of
coleoptiles and roots. However, in both cases a weak
tendency to stimulation of the coleoptile growth and
root growth inhibition was observed. Reduction of 15
nm GNPs concentration down to 10 µg/ml and 1
µg/ml had not effect on the growth of the seedlings,
but stimulated seed germination up to twofold.
Similar concentrations of 50 nm GNPs exerted the
stimulating effect on seed germination (twofold) and
the growth of root and coleoptiles. Seedlings grew
especially intensive in colloidal GNPs solution with
Au concentration of 10 µg/ml. Temperature rise of 2
degrees (from 25°C to 27°C) resulted in growth
increase of control 3-day-old seedlings and opposite
effect of 50 nm GNPs: coleoptiles and roots growth
fell behind control seedlings growth by 16-17%.
However, on the 4th day, the relative growth
slowdown of control seedlings occurred at 27°C and
growth stimulation effect under the influence of 50
nm GNPs appeared again. Over time, the stimulating
effect of 50 nm GNPs decreased: at the end of October it weakened, and in November – beginning of
December, it was no observed for the roots, and there
was a decline in coleoptiles growth. However, in all
cases the effect of stimulation of seeds germination
persisted under the influence of 50 nm GNPs,
weakening by December. We hypothesize the
molecular mechanisms of biological action of GNPs
In cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
We have described the theoretical assumptions and methodological solutions in the present review. They are innovative possibilities in the development of laboratory methods for assessing biological features of grain during the selection process
The article presents data on the morphological and biological characteristics of Agrodeum varieties, the results of the competitive test were shown, as well as the adaptability of varieties to different conditions of cultivation and the main indicators of quality of new grain varieties
This review is devoted to the 60 anniversary of the
winter wheat variety (Triticum aestivum L.)
Bezostaya 1 – a masterpiece of world selection. This
variety played a great role in increasing total grain
yields in the Kuban Region. It also was an important
gene source for Russian and world breeding. This
variety has initiated and was for many years a party
to research molecular biological mechanisms of
wheat frost resistance formation. The article
summarizes data about the peculiarities of
functioning of protein-synthesizing system it sprouts
(coleoptilies) under the influence of hardening
temperature (4oC): translational activity in vitro,
poliribosomts, degree of polyadenilation and stability
of total mRNA and specific mRNA translational
elongation factor α subunit 1 (eEF-1 α). The in vitro
(ommp) system, which adequately characterizes the
relative mRNA stability in vivo was used for these
measurements. The effects of cooling and light on
mRNA stability were shown to be cultivar-specific.
The hardiness of winter wheat has a positive
relationship with the amount of RNA and DNA, and
a negative correlation with the amount of magnesium
cations in mature grain that can serve as a simple
marker of frost resistance. The main feature of the
variety Bezostaya 1 is high content of magnesium
ions (Mg++) in RNA molecules and relatively low
variation of Mg++-depending molecularphysiological
responses for different environmental
conditions. The knowledge gained in the study of
variety of Bezostaya 1 contributes to the
understanding of molecular biological processes that
underlie the selection and determining its future
success