Scientific Journal of KubSAU

The key stage of clonal micropropagation, the micropropagation sensu stricto, affords to increase the propagation index for plants, especially perennials. In this study, we consider ways to optimize clonal micropropagation of remontant raspberry cultivars. It is found that microsprout regeneration rate is 78,5–96,0 % on the multiplication s. str. stage. The most multipurpose nutrient medium is selected with containing 0,5 mg/l both of BAP and GK. When one uses this medium, regeneration rate runs 79,0–94,0% depending on genotype with sprout high running 24,8–32,7 mm and sprout number – 1,2–2,2 per node. The optimal duration of growing cycle is 30 days with the propagation index within 6,6–7,8, depending of genotype

We have studied survival and regeneration rate of raspberries and blackberries buds when introducing into cultivation in vitro during October. The rate was significantly higher for buds taken in October than that for ones taken in February. At the first stage of micropropagation, for the most of studied varieties of raspberries, cultivation on nutrient medium contained BAP (0.5 mg/l) and GA (0.2 mg/l) was most successful; for blackberries, increasing concentration of hormones (BAP to 1.0 mg/l and GA to 0.5 mg/l) was better