Name
Koshchayev Andrey Georgiyevich
Scholastic degree
•
Academic rank
professor
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
—
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Articles count: 41
In the Krasnodar region there is a unique gene pool of animals both for dairy and meat. To assess the heifers the authors formed five experimental groups of animals, the most common species in the region. The authors studied the main economic, biological and exterior features: live weight, measurements, assessment of the exterior. The studies found that among intensive species with the largest body weight throughout the growing period, in 8-month old Charolais heifers superior Simmental ones at 17.4 kg. In one year old Simmental heifers were 9.2 kg (R˂0,05) ahead of Charolais and in 15 months – 10 kg (R˂0,05). Over the entire period of growth the highest rates of relative growth rates of heifers were obtained by red steppe breed type named Kuban, which had an advantage over the Aberdeen-Angus – 19.1%, Charolais – 5.6%, Hereford – 6.2%, and the difference between them and Simmental weren’t fixed. When growing, the animals become less tall, more stretched, wide and massive. We have noted that during the period of cultivation we had increased indices: stretch (9% – Red Steppe, 13.8% – Charolais, 16.1% – Hereford); chest (5.4% Aberdeen Angus). This reduces the index of long legs of red steppe – 5.6%, 10.9% for Charolais. The study of linear growth of animals confirms that the animal meat breeds of live weight increase was due to the extension of the body and increase the width of the pelvis and chest. Some different facts were observed in heifers of Red Steppe breed. For them, the most characteristic increase in the width of the chest was 1.44 times, as well as increasing the depth of the chest and chest girth behind the shoulders
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
The revolutionary discoveries of recent years in immunology has proved that protection from infections and biological aggression leading role played by innate immunity, which leads to the launch and subsequent work of specific adaptive immunity. Today, it is well-known that almost any pathology of the animal affects the immune system and creates an immune imbalance, leading to development of chronic pathological process, frequent relapses and to the development of different kind of complications. Secondary immunodeficiency states are widely distributed and differ in many etiological factors age, nutritional, medicinal, infectious and neoplastic nature, which leads to increased susceptibility of animal to various раthogenic factors, the weakening of the body. Awareness of the role of immunological disorders in the pathogenesis of a number of diseases has led to the need use in veterinary medicines that are aimed to influence on the immune system of the animal at the known molecular mechanism, to combine safety with high clinical efficacy. In this respect, a promising immunomodulators of microbial origin, due to the knowledge of the recognition mechanisms of the innate immune system such as bacteria as a whole, separate structural units, including mini-optimal biologically active fragments. This review is dedicated to one of the immunomodulator of microbial origin – which is an analog of biologically active fragment of muramyldipeptide of bacterial cell walls ‒ glucosaminylmuramildipeptide (GMDP), which is the active substance of registrated in Russia drug with the trade name Glicopin for use in veterinary practice. The drug showed high efficiency when used in cats, dogs, rodents, ferrets with infections of bacterial and viral etiology; cancer; with the aim of improving the natural resistance of the organism of young and old animals; enhance the effectiveness of vaccination in immunocompromised animals; postvaccine reduce complications; reduce stress financial position, such as during transportation and various veterinary treatments; injuries and on-line intervention (for stimulation of repair processes and tissue regeneration); prevention of immunodeficiency in newborn animals; the reduction of postpartum involutional processes in the uterus. Analysis of literature data allows us to consider an important outcome of immunologiс discoveries of recent years is the change of views on the question of the treatment of infectious diseases and the role of drugs as activators of innate immunity, among which an important role is played by a synthetic analogue of muramyldipeptide (GMDP) is a drug with a known molecular mechanism of action
In this study we show the results of the use of natural mineral compounds in metabolic disorders in cattle-breeding complexes of industrial type. According to official data for 2013-2015, based on the biochemical analysis of blood of cattle, that was held in some farms of Samara region, significant metabolic disorders were determined due to decrease in total protein level on 31,5% in average, decrease in alkali reserve – on 26,9% of samples, decrease in calcium concentration – on 40,7%, decrease of the phosphorus – on 8,1%, decrease in carotene – on 81,7% and glucose – on 84,3% of studies. Clinical examination of herd revealed a high level of animals with obvious signs of mineral metabolism disorders (osteodystrophy and rickets of alimentary origin) and comorbidity (nutritional anemia, gastroenteritis, pneumonia, disturbance of protein, carbohydrate and vitamin metabolism, and others). The use of complex natural compounds improves the efficiency of the traditional complex of treatment and prophylaxis, causing an increase in the total concentration of calcium in the blood serum on 10-55%, increase in calcium-phosphorus ratio till 1,27-2,3, increase in annual growth rate of young animals’ body weight on 8,2-44,8%, increase in milk production on 5,9-16,3%. Preparations have an antianemic action, normalize indicators of micronutrient metabolism of proteins and carbohydrates, enhance non-specific resistance factors and prevent the development of non-contagious internal pathology
We have studied biochemical changes in the body of
cattle, sick with nodular dermatitis in the initial stage
of the clinical manifestation of the disease. It is
proved that in case of disease in animals there is a
significant increase in the level of creatinine up to
295.5 mg%. The excess of the norm by 82.1%. In
treatment, this indicator was significantly reduced (189.2 mg%), but did not reach the upper limits of the
norm (162.4 mg%). In the blood serum of patients, the
carotene content was reduced to 0.09 mg%, which
was restored to normal three days after the start of
treatment (1.0 mg%). The glucose level at the
beginning of the disease was 18% below normal and
reached the lower limit of the norm 8 hours after the
start of treatment. When studying the activity of CKK,
it was found that the activity of the enzyme in animals
before the introduction of a 5% solution of sodium
hydrogencarbonate is 82% higher than the upper limit
of the norm. After the treatment, this indicator
returned to normal. The inorganic phosphorus index
did not reach the physiological norm (2.5 mmol / l)
before and after treatment, and remained 11.6%
higher (2.9 mmol / l), which indicates a violation of
mineral metabolism in sick animals. The level of
reserve alkalinity began to recover after 8 hours of
observation and reached a minimum limit of 48.91
vol. % CO2. During the entire period of the
experimental studies, before and after the
administration of the drug, there were no significant
changes in the biochemical parameters of total
protein, calcium, vitamin E and urea. The nature of
the established changes varied within the limits of
physiological norms. The use of a 5% solution of
sodium hydrogen carbonate in nodular dermatitis
increases the effectiveness of symptomatic treatment,
reduces the likelihood of complications and stimulates
the production of specific antibodies against viruses
and bacteria. We have recommended intravenous
injection of 5% sodium hydrogencarbonate solution to
sick animals at the rate of 1 ml per 1 kg of live weight
of the animal three times with an interval of 3 days
The work was done in the research laboratory of the
department of Biotechnology, Biochemistry and
Biophysics of Kuban State Agrarian University, the
aim of which was to select the most appropriate
nutrient medium for maximum growth of lactic acid
microorganism growth. The object of the study was its
own microflora of gastrointestinal tract of quails ‒
Lactobacillus agilis, Lactobacillus intermedius and
Lactobacillus salivarius. As a nutrient substrate there
were used 4 of the nutrient media: the medium for
lactic acid bacteria (city Uglich), the melasse-autolysis
medium, the glucose-peptone medium and the MRS.
During the cultivation of the microflora of the variable
parameters were the time and temperature of
cultivation. During the cultivation of microflora the
time and the temperature of growing were the variable
parameters. In the process of growing there was
carried out the analysis of dynamics of consumption of
reducing substances and titers of microorganisms. According to the results of growing of microbial
cultures there was revealed the active consumption of
carbon substrate in used variants of nutrient media,
and it was found that there was observed the maximum
of cells to 24 h of cultivation. On the basis of carried
out results of cultivation on different media and at
different parameters there was determined that the
most titer of cells was reached to 24 h at the
temperature 38,0 °С on the melasse-autolysis medium.
So, the melasse-autolysis medium can be
recommended in production conditions as the cheaper
substrate at the further development of biological
preparations for poultry breeding
The article reflects information characterizing the significance of mastitis and endometritis in the indicator of premature disposal of cows from herds of large farms in the western regions of the Krasnodar region: an average of 30% of the total culling of cows. The effectiveness of veterinary measures in Pravoberezhny JSC of the Temryuk district for mastitis and endometritis in cows was assessed: 81-95%; fertilization index value ‒ 1.8; the duration of the service period exceeds the requirements by 20 days. The studies the nature of the influence of the drugs called Sepranol and Uteroton on the calving process and the occurrence of postpartum complications in cows from the herd, which was formed with violation of breeding principles. The use of the drugs Uteroton and Sepranol contributed to a 50% decrease in the calving index in calving cows by 50% compared with the results of the combined use of estrophan and oxytocin. The use of Uteroton reduced the damage from the lack of milk by 87880 rubles, and the use of Sepranol - by 171164 rubles. We have given practical recommendation on the use of Uteroton and Sepranol for veterinary effects on the body of cows in the early postpartum period in order to reduce the occurrence of complications
The work was carried out in the scientific-research
laboratory of the chair of Biotechnology, Biochemistry
and Biophysics of Kuban SAU, the aim of which is the
search of optimal conditions of obtaining the
hydrolyzates of lactic acid bacteria by thermoacid and
enzymatic ways. The pure cultures of microflora of
gastrointestinal tract of quails − Lactobacillus agilis,
Lactobacillus intermedius and Lactobacillus salivarius
were the object of researches. Under conducting the
thermal acid hydrolysis there were used the same
correlations of bacterial mass and water as well as
procedures of clearing of hydrolyzates. The variable
conditions were рН, temperature and time of hydrolysis.
When carrying out the enzymatic hydrolysis in this
study there was used the enzyme from the group of
muramidase lysozyme. The amount of enzymes and
time of carried out hydrolysis were the variable
conditions under obtaining the enzymatic
hydrolyzates. In the process of researches there were
studied: qualitative content of total protein, peptides
with М.м. < 1500, protein and peptides with M.m. >
1500, aminoacids and as well as GMDP
(glucosaminilmuramilpentapeptide). On the basis of
carried out experiments there was stated that according
to all components of biologically active substances the
thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates
by different ways was revealed at destruction of cells
Lb. intermedius. Content of GMDP in thermal acid
hydrolyzate was higher than in enzymatic on
0,028 g/100 ml (51,8 %)
In this work, we have found shortened particle forms of the filamentous bacteriophage Pf, when the lysis of the Pseudomonas aeruginosa PAO1 strain was induced by extracts of bison feces to study the intestinal bacteriophages of this animal. The sizes of the shortened particles from 250 to 950 nm and their thickness 17 nm were determined. The particles had a slightly pointed one end and sometimes a flag on the other extended end. The particles were formed in the process lysis of this strain by other bacteriophages. These lysates also contained a high concentration of vesicles from the pseudomonad membrane. The article discusses the mechanisms of prophage induction in the Pseudomonas aeruginosa PAO1 strain due to phage-phage induction and substances of the investigated extract
The article demonstrates the results of therapeutic and preventive effectiveness of the use of lactic acid bacteria – Lactobacillus brevis and Lactobacillus parabuchneri in laboratory animals, in particular mice and rats. They were isolated from the gastrointestinal tract of a wild quail in experimental violation of the intestinal microbial background. Artificial imbalance of the intestinal microbiota of experimental animals was caused by the use of the antibiotic. As a result of tests it was established that the application of probiotic cultures improved microbial disorders of the gut, however, the use of their consortium in the probiotic promoted consistent clinical result characterized by an elevated level of representatives of the normal flora (lacto- and bifidobacteria) and reduction of the number of representatives of pathogenic and conditionally pathogenic microbiota, with subsequent achievement of a level characteristic of healthy animals