In the course of the work, 33 ISSR markers were
evaluated for efficacy in the detection of genetic changes
in regenerants of Galanthus woronowii Losinsk.. Ten
markers were found suitable for genotyping according to
the species under study. Five samples from the selected
ten were analyzed for a sample of 20 plants of
regenerants and a mother plant. The obtained data testify
to genetic stability of plant material in the process of
microclonal propagation
The study was aimed on approbation of 11 ISSR DNA-markers for their using in DNA fingerprinting of rare plant species of the Western Caucasus Lilium Caucasicum Miscz. Ex Grossh., Galánthus woronowii Kolak., Pancratium maritimum L. according to results DNA-markers set was selected for further using in the investigation of genetic diversity of the mentioned plant species
As the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
Results of testing of multiplex sets SSR-markers for
genotyping of rice varieties are presented in the article.
Two sets of SSR-markers were formed:
1: RM1+ RM11+ RM70+RM122;
2: RM164+RM167+RM168.
The optimal combination of DNA markers in the
multiplex sets and PCR conditions allowed obtaining
accurate, easily interpretable results when performing
fragment analysis on automated genetic analyzer
ABIprism3130. Using multiplex sets, genotyping was
performed for several varieties of rice: domestic
breeding and one variety – IR36 from the breeding of
IRRI (Manila, Philippines). For all the studied
varieties specific SSR-fingerprints were obtained.
RM 168 marker showed in domestic varieties a low
level of polymorphism - one allele of 97 bp. However,
at the same time, the variety IR-36, showed a second
type of allele 107 bp. In addition, the loci of RM1,
RM11, RM167 and RM164 have unique alleles in this
variety. It is consistent with significant genetic
differences of these varieties and the rest of the
varieties in studied sample. The proposed SSR
multiplexes are promising for use in DNA certification
of rice varieties and assessment of genetic diversity
Microsatellite DNA markers are currently used
effectively in the study of the genetic diversity of the
gene pool of fruit crops and DNA certification of
varieties. For plum now there is rather limited list of
works on the development of this type of DNA
markers. Most often, the search for new SSR-markers
for this species is carried out by checking of crossreproducibility
of SSR-markers developed in other
species of the genus Prunus. In this study, for the 18
SSR-markers previously developed on a peach, there
was performed testing and evaluation of the prospects
for the use of the genotyping of plum cultivars. Testing
was made on the 4 varieties of genetically distant,
belonging to the 4 different subspecies of Prunus
domestica L., showed the effectiveness of their use.
During the study, all tested DNA-markers were
grouped together in multiplex sets comprising 3-4
markers. This allows simultaneous genotyping of 3-4
loci in a single PCR reaction. These multiplex kits are
available for use in the study of genetic polymorphism
of species Prunus domestica L
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
This study was aimed on testing of IRAP markers developed
at plum on varieties of peach, Russian plum and cherry plum and genotyping of home and foreign varieties of plum, followed by an analysis of the data. During the testing of the markers have been identified as a high level of polymorphism between genotypes of plum and between the studied species. On the basis of the results obtained by genotyping 15 samples of Prunus was built dendrogram. Cluster analysis was divided into 3 groups corresponding varieties of P. persica, P. domestica and a common group for varieties of Russian plum and cherry plum
Professional apple gardening is bound to particular
risks, of which is essential losses of a harvest because
of diseases. An apple scab, the caused Venturia
inaequalis (Cooke) G. Winter, brings the greatest
loss. The main approach in monitoring of a scab of an
apple-tree is creation of grades, steady against
pathogen. In the present work we have carried out
phytopathology testing of generations of the seedlings
received from the free pollination of six forms of a
crab of Malus orientalis from a collection MOS VIR
(Maykop) characterized by a relative resistance to the
apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both
of natural population of a scab, and of strains of
pathogen of various cultivars and geographical origin.
When carrying out padding infection increase in force
of an infectious background that can be bound to
selection of the plants of biotypes of a fungus that are
most adapted for genotypes was noted. It is
recommended for precise elimination of unstable
plants at selection at early stages of an ontogenesis to
carry out more than one serial infection during the
season. By results of the phytopathological testing,
we highlighted three Malus orientalis forms from the
six studied No. 17982, 17985 and 3080 the most
perspective stability genes for an introgressiya in a
cultural gene pool of an apple tree
The article deals with the topical issues of ornamental
woody plants used in street landscaping of Sochi
Central district. It presents the quantitative
composition of these species, numbering 12181
specimens. With the scale, modified for the regional
conditions, we carried out an analysis of the studied
plantations and their state, which revealed that
weakened plants were predominant - 72.48%. Healthy
plants make up over 20%. The lowest number of the
specimen was recorded among the dead plants
(0,26%). The analysis was carried out for all species
according to the state categories. For instance,
structure-forming species selected for further studies,
were analyzed this way, including a large part of plants
that belong to the second category of state (89,04%).
Healthy plants are less than 8% from the total number
of dominant species. The number of dead plants makes
up 0,35%
The assessment results of urban environmental conditions on the basis of accumulation of heavy metals in the soil and vegetation are presented. The questions about the ability of different plants to accumulate heavy metals in the lives are discussed as well