Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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749 kb

APPROBATION OF ISSR DNA-MARKERS FOR GENOTYPING OF GALÁNTHUS WORONOWII LOSINSK.. AND ANALYSIS OF GENETIC STABILITY OF PLANTS, OBTAINED BY IN VITRO CULTURE

abstract 1331709088 issue 133 pp. 1166 – 1178 30.11.2017 ru 650
In the course of the work, 33 ISSR markers were evaluated for efficacy in the detection of genetic changes in regenerants of Galanthus woronowii Losinsk.. Ten markers were found suitable for genotyping according to the species under study. Five samples from the selected ten were analyzed for a sample of 20 plants of regenerants and a mother plant. The obtained data testify to genetic stability of plant material in the process of microclonal propagation
1481 kb

APPROBATION OF ISSR DNA-MARKERS FOR GENOTYPING OF RARE PLANTS SPECIES OF THE WESTERN CAUCASUS: LILIUM CAUCASICUM MISCZ. EX GROSSH., GALÁNTHUS WORONOWII KOLAK., PANCRATIUM MARITIMUM L.

abstract 1031409037 issue 103 pp. 611 – 623 30.11.2014 ru 1510
The study was aimed on approbation of 11 ISSR DNA-markers for their using in DNA fingerprinting of rare plant species of the Western Caucasus Lilium Caucasicum Miscz. Ex Grossh., Galánthus woronowii Kolak., Pancratium maritimum L. according to results DNA-markers set was selected for further using in the investigation of genetic diversity of the mentioned plant species
1588 kb

APPROBATION OF MICROSATELLITE DNA-MARKERS FOR IDENTIFICATION OF APPLE CLONES

abstract 0841210021 issue 84 pp. 262 – 271 28.12.2012 ru 1714
As the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
182 kb

APPROBATION OF MULTIPLEX SSRANALYSIS FOR DNA-FINGERPRINTS OF RICE VARIETIES

abstract 1141510103 issue 114 pp. 1420 – 1430 30.12.2015 ru 875
Results of testing of multiplex sets SSR-markers for genotyping of rice varieties are presented in the article. Two sets of SSR-markers were formed: 1: RM1+ RM11+ RM70+RM122; 2: RM164+RM167+RM168. The optimal combination of DNA markers in the multiplex sets and PCR conditions allowed obtaining accurate, easily interpretable results when performing fragment analysis on automated genetic analyzer ABIprism3130. Using multiplex sets, genotyping was performed for several varieties of rice: domestic breeding and one variety – IR36 from the breeding of IRRI (Manila, Philippines). For all the studied varieties specific SSR-fingerprints were obtained. RM 168 marker showed in domestic varieties a low level of polymorphism - one allele of 97 bp. However, at the same time, the variety IR-36, showed a second type of allele 107 bp. In addition, the loci of RM1, RM11, RM167 and RM164 have unique alleles in this variety. It is consistent with significant genetic differences of these varieties and the rest of the varieties in studied sample. The proposed SSR multiplexes are promising for use in DNA certification of rice varieties and assessment of genetic diversity
383 kb

APPROBATION OF SSR MARKERS DEVELOPED FOR THE SPECIES OF PRUNUS PERSICA, IN RELATION TO DISCHARGE PLUM

abstract 1241610098 issue 124 pp. 1479 – 1490 30.12.2016 ru 699
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
207 kb

APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS

abstract 1251701009 issue 125 pp. 151 – 163 31.01.2017 ru 566
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
259 kb

APPROVAL OF IRAP MARKERS BASED ON CASSANDRA RETROTRANSPOSON FOR THE ANALYSIS OF GENETIC POLYMORPHISMS IN THE GENUS PRUNUS

abstract 1041410055 issue 104 pp. 733 – 743 30.12.2014 ru 1167
This study was aimed on testing of IRAP markers developed at plum on varieties of peach, Russian plum and cherry plum and genotyping of home and foreign varieties of plum, followed by an analysis of the data. During the testing of the markers have been identified as a high level of polymorphism between genotypes of plum and between the studied species. On the basis of the results obtained by genotyping 15 samples of Prunus was built dendrogram. Cluster analysis was divided into 3 groups corresponding varieties of P. persica, P. domestica and a common group for varieties of Russian plum and cherry plum
200 kb

ARTIFICIAL SCAB RESISTANCE EVALUATION OF MALUS ORIETALIS FORMS – A POTENTIAL SOURCE OF NEW GENES FOR RESISTANCE TO APPLE SCAB

abstract 1311707113 issue 131 pp. 1377 – 1388 29.09.2017 ru 447
Professional apple gardening is bound to particular risks, of which is essential losses of a harvest because of diseases. An apple scab, the caused Venturia inaequalis (Cooke) G. Winter, brings the greatest loss. The main approach in monitoring of a scab of an apple-tree is creation of grades, steady against pathogen. In the present work we have carried out phytopathology testing of generations of the seedlings received from the free pollination of six forms of a crab of Malus orientalis from a collection MOS VIR (Maykop) characterized by a relative resistance to the apple scab in 2-3 classes of damage by long-term data. For infection we used inoculum, consisting both of natural population of a scab, and of strains of pathogen of various cultivars and geographical origin. When carrying out padding infection increase in force of an infectious background that can be bound to selection of the plants of biotypes of a fungus that are most adapted for genotypes was noted. It is recommended for precise elimination of unstable plants at selection at early stages of an ontogenesis to carry out more than one serial infection during the season. By results of the phytopathological testing, we highlighted three Malus orientalis forms from the six studied No. 17982, 17985 and 3080 the most perspective stability genes for an introgressiya in a cultural gene pool of an apple tree
166 kb

ASESSMENT OF STREET PLANTINGS ON THE EXAMPLE OF SOCHI CENTRAL REGION

abstract 1141510051 issue 114 pp. 687 – 697 30.12.2015 ru 793
The article deals with the topical issues of ornamental woody plants used in street landscaping of Sochi Central district. It presents the quantitative composition of these species, numbering 12181 specimens. With the scale, modified for the regional conditions, we carried out an analysis of the studied plantations and their state, which revealed that weakened plants were predominant - 72.48%. Healthy plants make up over 20%. The lowest number of the specimen was recorded among the dead plants (0,26%). The analysis was carried out for all species according to the state categories. For instance, structure-forming species selected for further studies, were analyzed this way, including a large part of plants that belong to the second category of state (89,04%). Healthy plants are less than 8% from the total number of dominant species. The number of dead plants makes up 0,35%
134 kb

ASSESSMENT OF ENVIRONMENTAL CONDITIONS ON THE BASIS OF HEAVY METAL CONCENTRATION IN URBAN SOIL AND VEGETATION

abstract 0811207032 issue 81 pp. 433 – 442 30.09.2012 ru 1628
The assessment results of urban environmental conditions on the basis of accumulation of heavy metals in the soil and vegetation are presented. The questions about the ability of different plants to accumulate heavy metals in the lives are discussed as well
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