Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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222 kb

DEVELOPMENT OF MULTIPLEX SETS OF SSR MARKERS FOR GENOTYPING APRICOT VARIETIES (PRUNUS ARMENIACA L.)

abstract 1441810013 issue 144 pp. 32 – 43 28.12.2018 ru 327
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.
104 kb

RECOMMENDATIONS FOR THE INSTALLATION OF PROTECTIVE PLANTINGS ALONG ROADS (SNOW BREAKAGE)

abstract 1451901011 issue 145 pp. 1 – 6 31.01.2019 ru 413
The significant part of our country's roads is subject to snowdrifts in winter. To ensure smooth and uninterrupted traffic in road transport during winter time, road facilities have to use vast amounts of mechanized and manual labor, as well as spend large sums of money. There are cases of snowdrifts on roads and interruptions in the traffic of road transport causing serious damage to the national economy of the country. Road workers face an important task of ensuring uninterrupted traffic in road transport in winter and reduce the cost of the road maintenance. In this regard, road facilities use a variety of means in order to protect motor roads from snow drifts: installation of portable shields, permanent fences, snow-protective plantings of various widths and structures. The evidence from practice shows that forest stand is the most durable, reliable and economical means of protecting roads from snowdrifts (snow breaking tree belts)
258 kb

HYGROSCOPICITY OF MATURE GRAIN AS A MARKER OF WINTER BARLEY AND SUNFLOWER FROST RESISTANCE

abstract 1451901012 issue 145 pp. 7 – 30 31.01.2019 ru 556
In the review article we present a comparative analysis of frost resistance of varieties of winter barley and sunflower (Helianthus annuus L.) by the results of freezing in the refrigerating chambers and by the hygroscopicity of mature grain. On the example of a number of varieties of both cultures, it is shown that the higher the frost resistance of the variety, the smaller the volume of the supernatant can be obtained by extracting the press cake with a solution containing magnesium cations. It is assumed that increased hygroscopicity of winter barley and sunflower press cake is associated with a relatively high content of so - called "water-soluble starch" - β-glucan polysaccharides in its grain. The possibility of regulation of the degree of hygroscopicity under the influence of potassium (KNO3), zinc (ZnSO4) and gibberellic acid (GA3) was studied on a number of sunflower varieties. It was shown that treatment with a solution of potassium (a concentration of 50 ppm KNO3) of sunflower plants at the stage of 4 leaves leads to a significant increase in hygroscopicity of grain and hardiness of plants, little effect was given treatment with zinc (30 ppm ZnSO4) and a lesser effect was observed when treating plants gibberellovaja acid (20 ppm). Potassium treatment significantly intensified the biosynthesis of free Proline, phenolic compounds and soluble proteins. When processing zinc significantly increased the content of carbohydrates in sunflower plants. Hygroscopicity showed a significant correlation with the content of free Proline (R2=0.621), phenolic compounds (R2=0.907), total carbohydrates (R2=0.673) and soluble proteins (R2=0.708). It is assumed that the treatment of plants with potassium and zinc plays a key role in increasing the hygroscopicity of grain. The results of comparative studies of frost resistance on the degree of survival of plants during freezing in cold rooms and the degree of hygroscopicity of mature grain showed that these two methods of assessing frost resistance give very close data. At the same time, in terms of simplicity and low economic costs, the proposed method of assessing frost resistance is many times superior to the method of direct freezing of plants
155 kb

THE STUDY OF THE RANGE OF TECHNICAL GRADES OF GRAPES IN THE ALUSHTA VALLEY

abstract 1461902011 issue 146 pp. 38 – 48 28.02.2019 ru 403
The analysis of varietal and age structure of vineyards occupied by 29 varieties and clones of the technical direction of use in the "Alushta" branch of FSUE "PJSC "Massandra". Taking into account the ecological and climatic conditions of the Alushta valley and the specialization of the economy, the largest share in the assortment of technical varieties is occupied by red grape varieties: Cabernet Sauvignon and clones of Cabernet Sauvignon R5, R8, VCR8, No. 169 including (26.68%), Bastardo Magarachsky and Bastardo clone VCR1 (12.06%), Saperavi (7.33%), Krasnostop zolotovsky (5.69%), Merlot and clone Merlot R3 (4.62 %). White varieties are in a smaller percentage: white Muscat and white Muscat clone R3 (8.47 %), white Kokur (7.1%), Aligote (3.82%), Semillon and clones Semillon № 173 and № 299 (3.68%), Gars level (2.99%). Plantings in the most productive age of 6-10 and 11-15 years are 57.5 % of the total area. At the same time, the share of introduced clones of varieties accounts for 26.65 %. In general, the age structure of the vineyards is favorable. The company produces high quality wine materials for production of traditional Federal state unitary enterprise "PJSC "Massandra" wines: white Port Alushta, Alushta red Port, pink Port Alushta, Alushta red Table, Pinot Gris, Massandra, Madera Crimean Alushta Tokay, white Muscat, Massandra Kokur, Semillon Alushta and sherry wine. In addition, there are new brands of semi-sweet white and red wines Pearl Massandra, as well as varietal wine materials Aligote, Cabernet, Sauvignon, Merlot, Saperavi, Chardonnay, Bastardo and Semillon. Analysis of the assortment shows that in the production plantings of "Alushta" branch of FSUE "PJSC "Massandra" there are varieties of medium and late ripening, which does not create peaks during harvesting and processing, ensuring uniform flow of raw materials for winemaking. It is necessary to expand the assortment of the economy due to a number of promising varieties of Syrah, Petit Verdot, Malbec, Sangiovese and others with a complex of economically valuable properties introduced from other regions
244 kb

TEMPERATURE REGIME OF THE TREATED SOIL LAYER

abstract 1461902012 issue 146 pp. 49 – 57 28.02.2019 ru 337
The article discusses the influence on the thermal regime of soil by agricultural activities, as well as the results of studies on determination of temperature in different soil layers under conditions of insufficient and unstable moistening of southern Russia. Experiments were performed in the experimental field in FSRI of the «ASC «Donskoy» (Zernograd, Rostov oblast) in terms of long-term stationary experience in the cultivation of soybeans with different technologies (traditional, zero). We performed the soil temperature checking at a depth of 30 and 60 cm using the temperature sensors with a measurement range from -40S to + 85S with a measuring accuracy of ±0,6S. For accumulation of data were used in the logger (weather station) Watch Dog 1400 series Micro. The purpose of the research was to determine and compare the influence of technologies of processing of soil on the temperature of the soil at different levels. On the basis of the conducted researches it is established that the average value of the ambient temperature stood at 23,8S a standard deviation of 3,1S. These figures are higher than the temperature of the soil at the background processed by traditional technology, and the raw background. The lowest average soil temperature at a depth of 30 cm (21,2S) recorded at zero background, obviously due to the presence on the soil surface of plant residues that protect the soil from solar radiation. Thus, the soil as a dynamic system converts the input signal (the ambient temperature) so that it is reduced in size and the rate of change (dynamic). These soil characteristics are determined by the technologies of soil processing
236 kb

PLANNING AND CALCULATION OF ORGANIC MINERAL FERTILIZERS DOSAGES IN FIELD CROP ROTATIONS WITH THE USE OF AUTOMATED INFORMATION SUBSYSTEM (BASED ON THE MATERIALS OF THE AGROINDUSTRIAL COMPLEX OF THE KRASNODAR REGION)

abstract 1461902013 issue 146 pp. 58 – 67 28.02.2019 ru 369
The problems of crop management and management decision support are still relevant at the moment. Crop production is of strategic importance and is the basis of our country's food security. The use of information technology in agriculture was limited to the use of computers and software, mainly for managing financial transactions and maintaining accounting and operational accounting. It should be noted that the measures taken to increase labor productivity and automate technological processes in the agro-industrial complex did not have an integrated nature; therefore, they slightly influenced the efficiency of the industry and, as a result, the competitiveness of products. Thus, the development and implementation of a computerized decision support system in crop management based on mathematical models for analyzing and evaluating the economic efficiency of crop cultivation technologies, crop rotation rationalization models, data analysis models of the book on crop field history, optimal selection of plant protection products and fertilizers highly relevant. This scientific project can be considered a priority and highly demanded, as contributing to the implementation of one of the requirements of the State program for the development of agriculture and regulation of the markets of agricultural products, raw materials and food of the Russian Federation for 2013-2020. The aim of the project is to improve the mathematical models and methods for managing technological processes at agricultural enterprises through the development and implementation of an integrated automated information system for crop management. The article is devoted to the theoretical substantiation and expediency of the practical implementation of the program module for solving the problem of planning and calculating the dosages of organic and mineral fertilizers in the field crop rotation of farms of the Krasnodar region, which is a part of the developed integrated automated plant management information system at agricultural enterprises
246 kb

ASSESSMENT OF THREATS TO THE VASCULAR PLANT DIVERSITY OF NATURAL COMMUNITIES AND OF REINEKE ISLAND FROM THE ALIEN SPECIES CENTAUREA JACEA L. (ASTERAСЕАЕ) (VLADIVOSTOK, PRIMORSKII KRAI, PETER THE GREAT BAY, SEA OF JAPAN)

abstract 1471903011 issue 147 pp. 21 – 30 29.03.2019 ru 301
In Reineke Island (Primorskii Krai, Vladivostok, Peter the Great Bay, Sea of Japan) alien species for Russia’s Far East Centaurea jacea L. (Asteraсеае) were first discovered in the 1980th in a small amount. Now, 40 years later distribution of C. jacea is limited to small sites of secondary grass meadows and disturbed habitats of roadsides unpaved roads for approximately 0.03% of the island; the level of its invasive activity is low; C. jacea threats currently is not for the biodiversity of plant community and forage value of meadows
228 kb

THE EFFECT OF FOLIAR TREATMENTS WITH MINERAL FERTILIZERS ON THE YIELD AND BIOMETRIC INDICATORS OF CUTTINGS

abstract 1471903012 issue 147 pp. 31 – 42 29.03.2019 ru 376
In this article, the influence of foliar treatments with complex fertilizers (Polimiks-agro, Sprühdünger 2) and a growth regulator (SeaweedMix) in the cultivation of rootstocks and seedlings given the concentration of drugs and phase of plant development. The positive effect of fertilizer "Polimix-agro" on physiological processes in plants of SC2U apple rootstock in the formation of adaptive resistance to abiotic stresses of the summer period, where the best water-holding capacity of the leaves than in the control. It was found that a single non-root feeding in the uterine growth regulator called SeaweedMix when reaching rootstocks height of 10-15 cm, allows to increase the yield by increasing the growth activity, increase the standard of layering with a decrease in the number of lateral branches, as well as 3-fold non-root fertilizing fertilizer Sprühdunger 2 provided an increase in the quality of rootstocks on the diameter of the trunk and height
199 kb

MAGNETIC PROFILES OF KUBAN SOILS OF VARIOUS AGRICULTURAL USE

abstract 1471903013 issue 147 pp. 43 – 52 29.03.2019 ru 239
The article discusses the change in the magnetic properties of soils of different agricultural use, depending on the conditions of soil formation. The objects of study are leached chernozem of Western Ciscaucasia, meadow chernozem and meadow bog soils of rice agrolandscapes. The results showed that with an increase in the degree of hydromorphism, the magnitude of the magnetic susceptibility of the soil decreases. The leached chernozem has the highest magnetization of the profile. In soils formed under the influence of hydromorphic soil formation, magnetic susceptibility is reduced by 3.0-3.5 times in rainfed conditions and deposits, and by 6.0-12.0 times in soils of rice agrocenoses
125 kb

INFLUENCE OF FERTILIZERS ON QUALITY OF CHARDONNAY GRAPES AND WINE MATERIALS

abstract 1471903014 issue 147 pp. 53 – 61 29.03.2019 ru 383
The article presents the results of the fertilizers use effect on dry wine materials produced from the Chardonnay variety, grown in the Anapo-Taman soil and climatic zone of the Krasnodar region, Russia. As a result of wine materials analysis, increase of tartaric acid was noted, with the autumn application of phosphorus-potassium fertilizers and the spring application of ammonium nitrate. At the same time, its optimum content was noted with nitroammophos. Also, the minimum concentration of malic acids was detected by the application of nitroammophos with potassium fertilizers. In its turn, early spring application of ammonium nitrate reduced the concentration of malic acid below the control sample (without fertilizers). It was revealed that the use of mineral fertilizers in this winemaking zone also contributed to a reduction of lactic acid in the must. At the same time, the application of nitroammophos (especially together with other mineral fertilizers), contributed to the accumulation of citric and succinic acids, as well as phenolic compounds. Moreover, the application of phosphate-potassium fertilizers in the autumn contributed to the accumulation, and, consequently, also to the increase in the content of phenolic compounds in the resulting must. In addition, the studied samples of wine materials contained high alcohol concentrations and low concentrations of sugars with volatile acids. At the same time, the maximum amount of alcohol exceeding its content in the control sample was revealed when phosphorus-potassium fertilizers were used together with nitroammophos fertilizers. It is also noted that the application of ammonium nitrate did not affect this indicator. As a result, the use of mineral fertilizers in the studied concentrations made it possible to prepare wine materials that contained a low concentration of sulfur dioxide, which is substantially lower than the harmful concentration for humans. In addition, increased concentrations of hydrogen ions in variants with the application of phosphate-potassium fertilizers have made it possible to reduce the number of iron tanates and the intensity of oxidation-reduction processes
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