Isolation of high-quality RNA from the tissues of perennial woody plants, including woody grape vines, is very difficult due to the high content of phenolic compounds, secondary metabolites and polysaccharides and the ribonuclease activity of destroyed tissues. Most of the existing methods require either large time or financial costs, or do not give reproducible results in the case of RNA extraction from mature grape tissues. The modified isolation protocol is based on a combination and modification of the known RNA extraction methods, taking into account the characteristics of mature grape tissues. Existing commercial kits for the isolation of RNA from plant tissues showed a low efficiency of RNA extraction from mature grape tissues, primarily associated with "varietal specificity". Reproducible results in the extraction of RNA showed CTAB-method, however, it has several significant drawbacks associated with the duration of the extraction and the complexity of the processing of an RNA preparation with a DNAase. The developed method is based on increasing the concentration of mercaptoethanol and polyvinylpyrrolidone in the extraction buffer, eliminating the stage of RNA selective precipitation via LiCl, and replacing it with deposition on a silica-based membrane (SiO2) followed by processing with DNA-ase. and increase the purity of the preparation of RNA from genomic DNA in comparison with the original method. A modified isolation protocol was developed based on a combination and modification of known RNA extraction methods, taking into account the characteristics of mature grape tissues. This solution allows to obtain reproducible quantity and quality of RNA for the subsequent synthesis of cDNA and RT-PCR
The results of the researches on studying of grape plantings processing influence of technical grade Viorika on its agrobiological and technological indicators are stated in the article. Experiences have been conducted in public joint-stock company "Pobeda" in Temrjuksky area in Krasnodar territory. The experience scheme included three variants in 2015: without processings (control); Farmaiodne - 0,04 %; Farmaiodne - 0,06 %; in 2016 - five variants: without processings (control); Farmaiodne - 0,04 %; Farmaiodne - 0,05 %; Farmaiodne - 0,06 %; Farmaiodne - 0,08 %. Processing of experimented plants included autumn and spring bushes washing in resting period (November-April) and spraying during the vegetation period. In 2015 spraying were conducted three times: before flowering, in a growth phase of berries, in the beginning of berries maturing phase; in 2016 the fourth spraying was added after the flowering. It is noticed, that application of Farmaiodne promotes more intensive accumulation of sugars in berries juice, acceleration of crop maturing, improvement of winematerials quality. The increasing of sprouts fruitfulness and increasing in average weight of a bunch is observed, that finally leads to authentic crop increasing in a bush and productivity. Application of Farmaiodne also has positive impact on growing processes, promoting increase in sprouts length and sometimes on thickness. According to the mixture of agrobiological and technological indicators the best results are received at the application in concentration of 0,05 % and 0,06%
Watermelon has great economic importance. The
fruits have high nutritional and medicinal value,
excellent taste, very healthy and are in great demand
among the population. Soil and climatic conditions of
the Temryuk district are favourable for growing
watermelon. High yield and excellent fruit quality in
the commercial led to the popularity of the Temryuk
watermelon not only on the black sea coast, but also
throughout Russia. The urgency of improving the
assortment of the watermelon is increased
competition among manufacturers, increasing
consumer culture of the population, the increase in
sales volumes, including due to the appearance on the
market of varieties and hybrids of non-traditional
colors of bark and pulp. The aim of our study was to
establish the most adapted hybrids of watermelon of
different segments, with high yield and marketability
of fruits, which are promising for growing in the
Temryuk district. Scientific novelty of our work lead
to the research objects – new, recently created
hybrids of watermelon, promising for cultivation in
the Krasnodar region. The work has great practical
significance, since on the basis of these studies
identified promising hybrids of watermelon foreign
seeds of various segments of the precocity, which
with appropriate cultivation agrobiological and
economic points of view
This article presents the results of a study of new me-dium-ripe lines obtained using the donor line Kl7427 for reduced grain harvesting moisture. According to the results of this study, the lines recommended as a starting material for the selection of lines and hybrids of maize of the middle group of ripeness with low grain moisture at the time of harvesting without signif-icant loss of productivity were identified
The article presents the results of studies on the biological characteristics of growth and fruiting, as well as the yield of adaptive varieties of strawberries in the conditions of the Prikubanskaya horticulture zone of the Krasnodar region. The objects of study are new and promising varieties of strawberries: Zenga-zengana (k), Onda, San-Diaz. The experiments were performed using the method of randomized reps. In the experiment there are 3 options, in each there are three replications of 20 plants. Variants and repetitions are placed sequentially. Agricultural records of the growth and fruiting of strawberry plants were carried out according to generally accepted methods; statistical processing of research results was carried out using the analysis of a variance method described by B.A.Dospehov. The results of our research showed that Zenga-zengana bushes (control) turned out to be the tallest. In the Onda variety, the height of the bush is 2 cm less than in the control variety; and San Diez varieties are 4 cm, respectively. It should be noted that the most undersized plants require a smaller area of nutrition. It has been established that flowers pollinated a few days after their disclosure develop more rapidly into berries than those that were pollinated immediately after opening. Pollen in flowers of the first order is less than in flowers of the third or fourth order. The stamens of the first flowers are often underdeveloped, their pollen is released and germinates worse. In our experience, the average weight of fruits and the yield as a whole depends on the biological productivity of the varieties. Onda was the most fruitful variety, and the rest varieties were at the control level
As the result of introduction research of Dagestan endemic Allium gunibicum under mountain conditions seed productivity variability data were obtained. An analysis of the data revealed significant difference in seed productivity performance of samples (inflorescence mass, seed mass, mass of 100 seeds, fruit number, ovules number, number of seeds, percentage of fruit bloom, seeds formation coefficient) among themselves both within one-year research and annually. The analysis showed that under the introduction with the same conditions the mean values of seed productivity characteristics decrease together with increasing of the sea level of collecting locations; at the same time, a mass of 100 seeds increases. The most variable factors were found out. They are seed mass in inflorescence, seed number in inflorescence, seeds formation coefficient. And the steadiest factors are a percentage of fruit bloom and mass of 100 seeds. As the result of the one-way analysis of variance a significant influence of the altitude above the sea level and slope disposition were discovered
The article presents a brief overview of the technologies for the production of wood-based pellets. The work gives disadvantages and advantages of the products manufactured using the surveyed technologies. A comparative analysis of the granules obtained by the considered technological processes is given. The conclusions are made about the economic and technological feasibility of using the presented technologies using wood waste as a raw material
The article presents experimental data of comparing anatomical and morphological features and properties of two peppermint varieties in the Hyaginsky district of the Adygea Republic. The economic importance of peppermint is very high. The essential oil rich in menthol, obtained from the leaves and mint colors, is widely used in the medical, perfumery, cosmetic and food industries. In this regard, of great value are varieties of a complex purpose - with high leaf productivity and high % essential oil and menthol.
Comparative analysis of morphological and anatomical features of peppermint raw material of peppery two varieties harvested in the territory of the Farm Sazonenko NV of Giaginsky district of the Adygea Republic, showed that the external features of plants differ from each other. The Kubanskaya 6 variety leaves have a larger size, with a higher content of essential oils, incl. menthol. In this regard, the yield of this variety was higher than that of Prilukskaya 6, and therefore the economic indicators of cultivation of the Kubanskaya 6 variety were the best
In this work we have given the analysis of the literary data confirming positive experience of impact of the hydrocarbon substance of growth (HSG) on viability of seeds, resilience, growth of plants, and productivity of various crops. It is shown that the basic experimental data belong to the 60th of the XX century. At the same time, works on application of HSG have been generalized for the first time in the collection of works of the Academy of Sciences of the Azerbaijani SSR by Institute of soil science and agrochemistry. HSG represents the by-product of petro processing received at sulphuric acid cleaning of diesel fuel with further processing with alkaline solution of NaOH. It includes mainly mixture of sodium salts of naphthenic acids. On the basis of literary data and own probes it is established that for each culture there is optimum very small concentration of HSG (0.005-0.01%), processing by solution of which makes positive impact on viability, growth and development of crops. The results of probes of blood formation of animals [8] and growth of mass of birds allow considering HSG ecologically safe. We have also presented the results of pilot study of impact of pre-seeding processing of seeds of wheat and peas by water solutions of the hydrocarbon growth substance (HSG) on their morpho-physiological parameters. The most effective concentration of HSG for processing of seeds of spring wheat of grade "Saratov" and grade peas "Alpha" is revealed. It is shown that the stimulator in concentration of 0.005-0.01% proves as stimulator of crop growth. It follows that degree of efficiency of action of HSG on improvement of development and increase in productivity of agricultural products from use of this drug is quite high. Considering that the stimulator can be received at insignificant expenses as a waste product of the purified diesel fuels, its application in agriculture is economically justified
The article presents organoleptic and physicochemical characteristic of baking wheat bread with 25% addition of flour of sorghum, and also wheat flour