Name
Suprun Ivan Ivanovich
Scholastic degree
•
Academic rank
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Honorary rank
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Organization, job position
All-Russian Research Institute of Rice
Web site url
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Articles count: 22
This article presents the results of testing IRAP DNA
markers Cass1 and Cass2 applied to Prunus spinosa.
The findings suggest the high perspectiveness of
their using for the study of genetic diversity of the
gene pool of this species. According to the results of
the analysis of the sample 12 genotypes were
identified from 6 to 13 fragments in the spectrum of
Cass1 and from 5 to 11 fragments for Cass2. As a
result of cluster analysis in the sample formed three
groups of samples. In one of the groups, which is
most distant from the other two, includes samples
taken in Ukraine, while the remaining two groups
included samples from Armenia, the Krasnodar
region region, the Republic of Adygea, Ukraine and
Moldova, and three cultural large-fruited form. The
distribution of samples in clusters corresponded to
their geographical origin that favors the objective
assessment of genetic distances between the samples
using Cass1 and Cass2 markers. Thus, it was
concluded that the prospects of using DNA markers
to study the genetic diversity within a species of
Prunus spinosa
Genetic studies of apricot are the actual direction in the genetics of fruit crops. In this regard, the improvement of the collection of SSR markers for the genotyping of this culture is an objectively significant task. In a study for the 16 SSR-markers previously developed on almonds (PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1, PdUnchar2, PdSLD1, PdGMGT1, PdTrTFGT1) and Siberian apricot (A3-72, A1-63, H2-22, A3- 7-1, H2-5, A1-7, A3-9, H2-45), approbation and evaluation of the prospects of using for genotyping Prunus armeniaca L. were performed. Approbation, performed on 3 varieties of different origin, revealed markers and their combinations optimal for their use. During the study, all tested DNA markers were grouped into multiplex sets, including 4 markers. This allows carrying out genotyping simultaneously on 4 loci in the formulation of one reaction. One marker (PdUnchar2) from the studied sample included in the multiplex set did not show amplification. Five markers gave a monomorphic product. The remaining 11 SSR markers allowed us to obtain polymorphic, cultivar-specific SSR fingerprints for all the studied cultivar. These multiplex sets are proposed for use in studying the genetic polymorphism of the species Prunus armeniaca L.