Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Tokmakov Sergey Vyacheslavovuch

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Honorary rank

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State Scientific Organization North Caucasian Regional Research Institute of Horticulture and Viticulture of the Russian Academy of Agricultural Sciences
   

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Articles count: 10

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788 kb

ANALYSIS OF ALLELIC POLYMORPHISM OF SELF-INCOMPATIBILITY IN SOME RUSSIAN VARIETIES OF PEARS (PYRUS COMMUNIS L.) USING CONSENSUS AND S5 AND S8, ALLELE - SPECIFIC DNA MARKERS

abstract 1031409036 issue 103 pp. 599 – 610 30.11.2014 ru 541
In the process of the research we had accomplished an analysis of allelic polymorphism of self-incompatibility in pears in demand in the production and breeding of modern varieties of pears of North-Caucasian Zonal Research Institute of Horticulture and Viticulture collection of genetic resources. In the first stage the consensus primers PycomC1F and PycomC5R were used. With obtained data, after identification of the alleles studied in varieties using consensus primers an allele specific S5 and S8 DNA markers were used for confirming the presence / absence of data allele studied cultivars
382 kb

APPROBATION OF GENOTYPING METHOD OF WINE YEAST (GENUS SACCHAROMYCES) BY THE ANALYSIS OF INTER-DELTA GENOMIC REGION

abstract 1121508036 issue 112 pp. 481 – 491 30.10.2015 ru 549
The study was performed to genotype some commercial wine yeast strains using the assay of Interdelta genomic sequences. Experimental parameters of PCR to identify were optimized and optimal simplified method of DNA extraction from dried preparations of yeast cultures was define. Proven method showed a high level of resolution and can be used for the analysis of genetic diversity wine yeast in combination with SSR-markers
1588 kb

APPROBATION OF MICROSATELLITE DNA-MARKERS FOR IDENTIFICATION OF APPLE CLONES

abstract 0841210021 issue 84 pp. 262 – 271 28.12.2012 ru 1093
As the result of the work, the test of microsatellite DNA markers for the identification of genomic polymorphism between apple cultivars Florina, Golden Delicious and clonal forms has been done. With the high level of intervarietal polymorphism, studied DNA markers showed no allelic differences between varieties and their clonal forms
383 kb

APPROBATION OF SSR MARKERS DEVELOPED FOR THE SPECIES OF PRUNUS PERSICA, IN RELATION TO DISCHARGE PLUM

abstract 1241610098 issue 124 pp. 1479 – 1490 30.12.2016 ru 235
Microsatellite DNA markers are currently used effectively in the study of the genetic diversity of the gene pool of fruit crops and DNA certification of varieties. For plum now there is rather limited list of works on the development of this type of DNA markers. Most often, the search for new SSR-markers for this species is carried out by checking of crossreproducibility of SSR-markers developed in other species of the genus Prunus. In this study, for the 18 SSR-markers previously developed on a peach, there was performed testing and evaluation of the prospects for the use of the genotyping of plum cultivars. Testing was made on the 4 varieties of genetically distant, belonging to the 4 different subspecies of Prunus domestica L., showed the effectiveness of their use. During the study, all tested DNA-markers were grouped together in multiplex sets comprising 3-4 markers. This allows simultaneous genotyping of 3-4 loci in a single PCR reaction. These multiplex kits are available for use in the study of genetic polymorphism of species Prunus domestica L
207 kb

APPROBATION OF SSR-ANALYSIS FOR DNA-IDENTIFICATION OF COMMERCIAL WINE YEAST STRAINS

abstract 1251701009 issue 125 pp. 151 – 163 31.01.2017 ru 228
The study was performed to genotype some commercial wine yeast strains with SSR-markers. Five polymorphic SSR-markers were tested in a selection of 15 yeast strains. Tested SSR-markers showed a high level of informativeness as well as polymorphism and can be used further to analyze the genetic diversity of wine yeast
259 kb

APPROVAL OF IRAP MARKERS BASED ON CASSANDRA RETROTRANSPOSON FOR THE ANALYSIS OF GENETIC POLYMORPHISMS IN THE GENUS PRUNUS

abstract 1041410055 issue 104 pp. 733 – 743 30.12.2014 ru 586
This study was aimed on testing of IRAP markers developed at plum on varieties of peach, Russian plum and cherry plum and genotyping of home and foreign varieties of plum, followed by an analysis of the data. During the testing of the markers have been identified as a high level of polymorphism between genotypes of plum and between the studied species. On the basis of the results obtained by genotyping 15 samples of Prunus was built dendrogram. Cluster analysis was divided into 3 groups corresponding varieties of P. persica, P. domestica and a common group for varieties of Russian plum and cherry plum
4917 kb

DEVELOPING OF INSIDE GENE MOLECULAR MARKERS OF RICE FOR INCREASING OF BREEDING AND SEED PRODUCTION PROCESSES EFFICIENCY

abstract 0671103019 issue 67 pp. 283 – 292 30.03.2011 ru 1438
Molecular markers for allele identification of breeding important rice genes Pi-ta (blast resistance) and Rc (red pericarp) have been created. Their efficiency is also checked
740 kb

IDENTIFICATION OF S2 AND S3 ALLELES OF SELF-INCOMPATIBILITY GENE IN CRAB APPLE AND ADVANCED BREEDING SELECTIONS

abstract 1321708084 issue 132 pp. 1076 – 1085 31.10.2017 ru 82
Industrial horticulture assumes the most effective use of the potential of varieties. One of the key factors determining the yield of garden plantings is the effectiveness of pollination. To obtain the maximum yield, it is necessary to ensure maximum pollination during the flowering period. For this reason, much attention has been paid to the selection of pollinators. Crab-apple forms are promising for use as pollinators, so this work was aimed at identifying the most common alleles of the self-incompatibility gene in the crab-forms using the molecular genetic method of analysis. The object of the study was 29 apple-tree creams and 3 elite selection forms. They carried out the molecular genetic identification of alleles S2 and S10, which are among the most common apple trees in the world gene pool. Allele S2 was identified in 16 samples (14 forms and 2 elite selection forms), while S10 allele in one sample (elite form 12/2-20 (24-28)). Data on the allelic composition of the S gene in the samples studied are of value for the formation of a genetic passport on the compatibility of the studied samples of apple with modern industrial varieties
246 kb

MOLECULAR-GENETICS BASE OF APPLE SELF-INCOMPATIBILITY

abstract 0801206025 issue 80 pp. 318 – 327 30.06.2012 ru 1112
Estimation of self-incompatibility allelic diversity in Russian apple varieties was conducted. We have established S-allele gene set for a number of varieties. The most common allele of gene was identified. Alleles frequency data obtained are consistent with those in the world apple germplasm
295 kb

ДНК-ПАСПОРТИЗАЦИЯ СОВРЕМЕННЫХ РОССИЙСКИХ СОРТОВ РИСА С ПРИМЕНЕНИЕМ SSR-МАРКЕРОВ

abstract 1311707065 issue 131 pp. 772 – 782 29.09.2017 ru 125
In the presented study, we have performed genotyping of modern Russian rice cultivars using microsatellite DNA-markers. The markers showed different level of allelic polymorphism: from 2 to 8 alleles per locus. For all studied cultivars,unique DNA-fingerprints were obtained
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