Name
Zvyagin Andrey Sergeyevich
Scholastic degree
•
Academic rank
—
Honorary rank
—
Organization, job position
Kuban State Agrarian University
Web site url
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Articles count: 15
In this article we have described our work on genotyping of new ten productive wine grape protoclones
In this article we have described a study of genotyping of new three productive wine grapes protoclones: Rhine Rieslin, Verdot black and Johanniter
The article presents the results of studies of 92
indigenous varieties and 15 wild genotypes by 24
microsatellite markers. As a result a locus database about
germplasm in the collection stored at the Russian
ampelographical collection AZOSViV (Anapa Zone
Experimental Station of Viticulture and winemaking)
was created. During the research of varietal breeding
populations confirmed the validity in the production of
the cultivar of Krasnostop anapsky with obvious changes
in microsatellite sequences as a clone of famous indigenous
Don cultivar Krasnostop Zolotovskii. This clone
was selected in 1953 by prominent agrotechnologist
Zotkin Ivan Ivanovich which is propagated by the
author and his followers, and after a formal registration
submitted to state testing in 2007 in GSK RF, zoned in
the North Caucasus region (number 6) in 2014 year. Sort
-clone Krasnostop anapsky only in the Kuban today
cultivating in 35 hectares. By using molecular genetic
techniques, now clonal selection of vine grapes is reasonably
improving. Recent data collected by us in the
work process, were analyzed a comprehensive manner,
making it possible to draw conclusions about distinctness
of grape varieties. Some clones were studied by DNA
primers, based on retrotransposon sequences
The article presents the structural and phylogenetic
analysis of VIT_02s0033g00410,
VIT_02s0033g00390 and VIT_02s0033g00450
genes of the grapevine genome and the closely
related orthologous gene MYB114 of the
Arabidopsis genome. These genes are responsible
for the biosynthesis of anthocyanin in the organs of
model plants and are of interest not only for practical
production and breeding, but also for fundamental
research. These genes were analyzed for GCcomposition
of nucleotides, the presence of cisregulatory
elements and promoter regions. DNA and
protein sequences were aligned to look for similar
elements, which allowed further analysis of the
ultraconservative domains of four genes. Based on
the results of search and identification of the
conservative regions, a cluster tree was constructed,
which made it possible to identify the separation of
gene sidelines from, presumably, the main one. At
the same time, the construction of consensus trees
based on DNA and protein sequences revealed their
absolute similarity. "The Minimal Evolution Tree"
allowed calculating the approximate dates of the
appearance of the mutations and the divergence
times of the gene branches between each other. At
the same time, the appearance of the Vitis genus and
its separation from the Rosales was taken as the time
first divergence point. In the end, homologous
metabolic pathways were searched between
grapevine and Arabidopsis, which revealed the
presence of homologous proteins in the grape
proteome. In this turn, it already confirms the
existence of similar biosynthetic pathways and, as a
consequence, interactions such as "DNA-protein"
and "protein-protein"