Name
Butanaev Aleksandr Mikhailovich
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
Institute of Basic Biological Problems
Web site url
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Articles count: 1
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855