Scientific Journal of KubSAU

Polythematic online scientific journal
of Kuban State Agrarian University
ISSN 1990-4665
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Name

Plotnikov Vladimir Konstatinovitch

Scholastic degree


Academic rank

associated professor

Honorary rank

Organization, job position

Kuban State Agrarian University
   

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Articles count: 17

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MOLECULAR-BIOLOGICAL ADAPTATION MECHANISMS OF PROTEIN-SYNTHESIZING SYSTEM IN ANIMALS TO IMBALANCED AMINO ACIDS

abstract 0861302032 issue 86 pp. 432 – 471 28.02.2013 ru 1175
In the article, there are the results of comparative studies of the effect of diets with imbalance lysine and tryptophan on the growth, appetite and tissue components of the protein-synthesizing system of Wistar rats and pigs
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GENE EXPRESSION OF ANIMALS IN THE AMINO ACID IMBALANCE Part I

abstract 0881304018 issue 88 pp. 252 – 273 30.04.2013 ru 1491
This review covers the advances of the molecular biology in the study of gene expression characteristics of animals in the amino acid imbalance
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ANIMAL GENE EXPRESSION IN THE AMINO ACID IMBALANCE. Part II

abstract 0881304019 issue 88 pp. 274 – 314 30.04.2013 ru 1131
This review covers the advances of the molecular biology in the study of gene expression characteristics of animals in the amino acid imbalance
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BIOCHEMICAL IMPACT OF OPAQUE-2 REGULATORY GENE MUTATION IN HIGH-LYSINE CORN KERNEL: 50 YEARS OF INVESTIGATION

abstract 0981404088 issue 98 pp. 1225 – 1259 30.04.2014 ru 1353
The main aim of this article is to generalize the results of biochemical and molecular research of developing opaque-2 maize seeds (high-lysine mutant of maize) metabolism peculiarities
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BIOLOGICAL MARKERS FOR SELECTION ON THE FROST RESISTANCE OF WINTER WHEAT AND BARLEY FORM

abstract 1041410128 issue 104 pp. 1834 – 1866 30.12.2014 ru 1406
We have described the theoretical assumptions and methodological solutions in the present review. They are innovative possibilities in the development of laboratory methods for assessing biological features of grain during the selection process
190 kb

CYCLICAL EFFECTS OF ACTINOMYCIN D ON THE BARLEY COLEOPTILES GROWTH

abstract 1071503088 issue 107 pp. 1342 – 1361 31.03.2015 ru 1025
In cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
276 kb

60 YEARS IN THE RANKS: FEATURES OF MOLECULAR BIOLOGY OF BEZOSTAYA 1 WINTER WHEAT VARIETY

abstract 1181604035 issue 118 pp. 627 – 657 29.04.2016 ru 828
This review is devoted to the 60 anniversary of the winter wheat variety (Triticum aestivum L.) Bezostaya 1 – a masterpiece of world selection. This variety played a great role in increasing total grain yields in the Kuban Region. It also was an important gene source for Russian and world breeding. This variety has initiated and was for many years a party to research molecular biological mechanisms of wheat frost resistance formation. The article summarizes data about the peculiarities of functioning of protein-synthesizing system it sprouts (coleoptilies) under the influence of hardening temperature (4oC): translational activity in vitro, poliribosomts, degree of polyadenilation and stability of total mRNA and specific mRNA translational elongation factor α subunit 1 (eEF-1 α). The in vitro (ommp) system, which adequately characterizes the relative mRNA stability in vivo was used for these measurements. The effects of cooling and light on mRNA stability were shown to be cultivar-specific. The hardiness of winter wheat has a positive relationship with the amount of RNA and DNA, and a negative correlation with the amount of magnesium cations in mature grain that can serve as a simple marker of frost resistance. The main feature of the variety Bezostaya 1 is high content of magnesium ions (Mg++) in RNA molecules and relatively low variation of Mg++-depending molecularphysiological responses for different environmental conditions. The knowledge gained in the study of variety of Bezostaya 1 contributes to the understanding of molecular biological processes that underlie the selection and determining its future success
287 kb

INTERACTIONS OF GOLD, SILVER AND MAGNESIUM NANOPARTICLES WITH PLANTS

abstract 1201606047 issue 120 pp. 675 – 705 30.06.2016 ru 667
The review examines the recent years’ data pertaining to the interaction of plants with nanoparticles of noble metals (gold and silver) and with nanoparticles of magnesium as an element that has a central role in the organization of the cellular protein-synthesizing apparatus. Information is presented about the effect of gold and silver nanoparticles on plants and on possible nanoparticle phytotoxicity. It is shown that magnesium nanoparticles, as well as those of noble metals, produce various effects on the growth and productivity of plants, enabling the arsenal of biologically active substances to be expanded. Analysis is made of the involvement of magnesium nanoparticles in cryobiological reaction of the regular breakdown of plant RNA under conditions of ultra low temperatures, which is important not only for the further developments of views of the origin of life ob Earth but also for the purposes of designing new markers for the analysis of the biological peculiarities of agricultural crops on the basis of studies of magnesium in the long-lived RNA of their mature seeds. The presented critical analysis of the literature data, which are often incomplete and contradictory, suggest that a coordinated research program is needed that would detect correlation between particle parameters, experimental design, and observed biological effects
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SPECIFICITY OF TRILON B INFLUENCE ON THE GERMINATION OF SEEDS OF VARIETIES OF WINTER BARLEY

abstract 1201606048 issue 120 pp. 706 – 729 30.06.2016 ru 401
The problem of increasing resistance of winter barley to adverse conditions of winter is the most problem in the selection area of this crop. The main component of this complex trait is the frost resistance. Laboratory methods of assessment of frost resistance are important for breeding of winter barley. These methods can effectively replace more costly method of direct freezing in the freezers. Magnesium cations (Mg++) are essential components of the protein-synthesizing system of germinating seeds. Magnesium cations determine the stability and translational activity of mRNA and rRNA. Trilon B is usually used for in vitro banding of magnesium cations in area of biochemistry of the nucleic acids. It was very interesting to verify the Trilon B influence to seed germination of winter barley varieties by experiment. Experiments showed: actinomycin D – the inhibitor of RNA synthesis – didn’t show varieties specific effect to seed growth of winter barley, while Trilon B had a varieties specific impact to length coleoptiles and roots. Research was carried out on etiolated seedlings at temperatures from 22 to 28oC, and different concentrations of Trilon B - 1,6×10-3М, 2,4×10-3М and 3,2×10-3М. The roots of 3-days old seedlings were more sensitive to Trilon B influence. It is shown the varieties of Russian origin were naturally reacted to Trilon B: the higher the frost resistance of variety, the more resistant variety to the action of various concentrations of Trilon B at different temperatures. The most optimum concentration was 3,2 × 10-3М at 22-25oC. Varieties by foreign origin deviated from this pattern. It is interesting to research the genetic essence of these differences. It will be helpful for optimization of varieties differentiation conditions to create a laboratory method of estimate of frost resistance of winter barley. The new method will be most efficient compared the direct freezing method
164 kb

EFFECT OF GOLD NANOPARTICLES ON SEEDS GERMINATION OF WINTER BARLEY

abstract 1271703018 issue 127 pp. 295 – 307 31.03.2017 ru 458
The article presents experimental data on the research of gold nanoparticles (GNPs) with mean diameter 15 nm and 50 nm effect on seeds germination and growth of 3-day-old etiolated coleoptiles and roots. Compared with distilled water (control) initial colloidal solution of 15 nm and 50 nm GNPs (57 µg/ml) had no significant impact on seeds germination and growth of coleoptiles and roots. However, in both cases a weak tendency to stimulation of the coleoptile growth and root growth inhibition was observed. Reduction of 15 nm GNPs concentration down to 10 µg/ml and 1 µg/ml had not effect on the growth of the seedlings, but stimulated seed germination up to twofold. Similar concentrations of 50 nm GNPs exerted the stimulating effect on seed germination (twofold) and the growth of root and coleoptiles. Seedlings grew especially intensive in colloidal GNPs solution with Au concentration of 10 µg/ml. Temperature rise of 2 degrees (from 25°C to 27°C) resulted in growth increase of control 3-day-old seedlings and opposite effect of 50 nm GNPs: coleoptiles and roots growth fell behind control seedlings growth by 16-17%. However, on the 4th day, the relative growth slowdown of control seedlings occurred at 27°C and growth stimulation effect under the influence of 50 nm GNPs appeared again. Over time, the stimulating effect of 50 nm GNPs decreased: at the end of October it weakened, and in November – beginning of December, it was no observed for the roots, and there was a decline in coleoptiles growth. However, in all cases the effect of stimulation of seeds germination persisted under the influence of 50 nm GNPs, weakening by December. We hypothesize the molecular mechanisms of biological action of GNPs
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