The article presents results of the study of lavender reproduction (Lavandula angustifolia Mill) with methods of generative reproduction (seeds), vegetative reproduction (cutting, layening, divide of buch), clonal microreproduction using the culture in vitro.
The method of cloned microreproduction based on the culture of the isolated meristems which provides genetic identity of regenerated plants to initial forms and high coefficients of reproduction, improvement of landing material from fungal and bacterial infection, and also release from viruses at a combination with methods of thermotherapy and a chemotherapy is the most effective for the solution of objectives of seed farming of a lavender. As a material for carrying out researches we had plants of lavender (Lavandula angustifolia Mill), Sineva sorts, Stepnaya and perspective selection samples 337-9 and 310-17. Apical meristems 0,2-1,0 mm high isolated from top and axilary buds of a stalk of annual plants have been used as explant. Murasige and Skuga (MS) used as a basic nutrient medium for cultivation of the isolated meristems. Explants have been cultivated in the cultural room with 25-26 0C - temperature, lighting – 2-3 lx, relative humidity – 60-70%. It has been established that both April and October are the best month for isolation of meristems correspond calendar to phases of spring and autumn growth at the lavender donor plants and that optimum is an agar nutrient medium of MS, added with kinetin (1,0 mg/l) and GC (1,0 mg/l); frequency of regeneration of all studied genotypes was 90,0-100,0 %. Feature of morphogenesis of lavender meristems in vitro culture was already at the first stage of clonal microreproduction as there was a multiple shoot formation. Studying of features of development of lavender microplants during ten passages also was carried out, as the level of stability of regeneration processes throughout several cycles of a miсrograftage is one of important factors on which efficiency of microreproduction depends. We have also shown, that the efficiency of reproduction remains at the stable level of the sort named Sineva and sample #337-9 to the 8th passage (1:7,77-12,45 and 1:7,60-11,85 respectively), at the variety called Stepnaya - to the 7th passage (1:6,10-11,81), at the sample #310-17 - to the 6th passage (1:6,17-8,37)
In order to obtain fortified feed additives and malt the use of activated water solutions was studied
Large agricultural load on the steppe landscapes
leads to the transformation of ecosystems and effect
on all their components. Transformation of lands into
laylands leads to a gradual restoration of
anthropogenically disturbed steppe ecosystems.
Studies that focus on the research of comprehensive
processes in the territory of laylands are extremely
relevant today. Herpetobiont fauna is one of the
important characteristics of ecology and biology of
soils. Herpetobiont Coleoptera can serve as
indicators in the study of processes occurring in
postagrogenic soils.The article presents the results of
the study of changes in mesofauna, composition and
structure of complexes of the gerpetobiont
Coleoptera of ordinary black soils in natural
succession series of different-age laylands. We also
evaluated the degree of faunistic similarity of plots
of laylands and allocated dominant species.
Carabidae and Tenebrionidae are the basis of
complex Coleoptera in the investigated laylands. The
article shows that such indicators as the number of
individuals, species, families of beetles and their
dynamic density are depended on the age of the
layland. In addition, the indices of biodiversity
(Margalef and Shannon) and dominance (Simpson)
are also connected with the age of plots of laylands
The article studies the influence of polymeric in
the form of formers and growth regulators on the
growth and development of rice plants when
grown in saline conditions. We controlled the effect
of salinity on grows regulators and vigor, seed
germination, root and shoot weight, the content of
photosynthetic pigments parameters, induction
curves of delayed fluorescence, the indicators of
structure of harvest, grain yield. It was found, that
pre-sowing seed soaking in solutions of polymer
grows regulators has a stimulating effect on the
growth and development of rice plants in the early
stages: we significantly increased germination and
emergence, dry weight of root and shoot compared
to control. At different stages of ontogeny
rice, the absolute content of pigments in the leaves
and the relationship between the individual variants
change. The absolute content of pigments in
leaves and their relationship between experiences
at different stages of ontogeny change. In the period
of intensive vegetative growth from seedling
stage the content of total chlorophyll is maximum,
and by the end of the growing season it decreases.
It can be assumed that the salinity of the substrate
significantly reduces the productivity of photosynthesis
in young plants, possibly due to imbalance
of ions in the cell, the older it gets – the weaker
the phenomenon is and even becomes reversed.
The second maximum IR ZF increases during the
growing season from germination to flowering,
then decreases to the beginning of ripening in all
embodiments. The same dynamics is characteristic
of the magnitude of the proton gradient in the
membranes of chloroplasts tylakoids. Salt protection
effect of growth regulators on grain yield is
significant on both backgrounds of mineral nutrition
In the article we have investigated the influence of nickel oxide in the amount of 100, 1000 mg/kg of the soil (1, 10 MPC), combined with the influence of an alternating magnetic field of induction of 50, 100 and 650 µT power frequency of 50 Hz on the biological
properties of alkalinity black soil
The researches have been conducted for the optimization of culture medium for unique genotipes of Populus tremula L. in vitro. It was found that for induction of proliferation of the arms of Populus tremula in vitro one should use MS culture medium with 0,1 ml / l IBA and 0,1 or 0,5 ml / l BAP. For stimulation of rhizogenesis MS culture medium with 0,3 ml / l IBA should be used
We studied the effects of heavy metal pollution on
soil phytotoxicity cities of the Rostov region. The
research objects were the soils of urbolandscape
towns: Taganrog, Novocherkassk, Gukovo, Shakhty,
Azov. The study of the soil pollution with heavy
metals was recorded in Gukovo, Shakhty, Taganrog.
The degree of pollution of the studied cities form the
following row: Gukovo > Shakhty > Taganrog >
basics > Novocherkassk. The research was carried
out in 2011-2015 at the Department of ecology and
environmental Sciences of southern Federal
University. In most cases, there was a direct
correlation between pollutant concentration and the
degree of deterioration of the studied soil properties.
Used biological indicators are informative for
monitoring of urban soils polluted with heavy metal.
The degree of informativity of biological indicators
form the following row: root length > germination
>length of shoots. The results of the study can be
used for monitoring and diagnosing the condition of
contaminated soils, in the assessment of
environmental impact, risk assessment of natural and
synthetic disasters, the development of regional
standards on the content of heavy metals in soil and
in other environmental and industrial activities
Results of researches of influence of anthropogenic factors on the condition of tree plantations in Maykop
The article studies the impact of different substrats on rizoplane microflora of pelargonium zonale in protected ground
The results of the study presented showed that predator mite Ph. persimilis exhibited high food preference of the larval stage (30,2% of the total number consumed) compared to the egg (20,5%), nymph (13,4%) and adult (10,1%) stages of two spotted spider mite T. urticae. The functional response of Ph. persimilis feeding on eggs and adults of T. urticae was described by the Type II curve. At a maximum density of 60 prey per leaf disc, Ph. persimilis consumed an average of 22.8 eggs and 3.2 adults over an eight-hour period. Calculated time for handling of the prey by Ph. persimilis was on the average 0.074 hours (4.4 minutes) and 3.2 hours for T. urticae eggs and adults respectively. The mean number of T. urticae eggs consumed by Ph. persimilis progressively increased with the increase of the prey density, but the rate of predation (number of eggs consumed/prey density) decreased from 0.5 to 0.3 with the increasing of prey density. The mean number of adult T. urticae consumed by Ph. persimilis at densities of 20, 40 and 60 per leaf disk remained relatively constant. But the rate of predation decreased from 0.09 to 0.04 with increasing of prey density. The experiments showed that regardless of the strategy utilized by the predator it was the one that was efficient for the predator survival