The results of genetic variation and differentiation study of adjacent populations growing on swamp and on dry land was done using examine of ISSR DNA regions. The same diversity level and comparatively high division were shown in this article
The article presents a comparative analysis of the sanitary condition of green plantings in park area and street network of trees of local forest origin and exotic species
The article provides an overview of materials of conferences on problems and prospects of the industry of cattle breeding
In the article, there are the results of comparative studies of the effect of diets with imbalance lysine and tryptophan on the growth, appetite and tissue components of the protein-synthesizing system of Wistar rats and pigs
A method of obtaining insertion mutants for the hoc
gene, which encodes for the main phage antigen, was
developed on the model of bacteriophage T4. This gene
was cloned in the plasmid pBSL0+ and was disrupted
by insertion of foreign DNA. The phage mutants were
obtained by in vivo phage-plasmid recombination. The
construction of insertion bacteriophage mutants was
carried out in two stages. The resulting mutants on this
procedure could be grown on wild-type E. coli strains,
which is convenient for the production and use of these
phages in therapy. The mutants obtained had reduced
antigenicity. At the same time, the yield of the mutant
strains was high when they were grown on the
non-suppressor E. coli laboratory strains. A number of
stages of purification of the bacteriophage mutants
obtained were performed. Preparations have been
studied by transmission electron microscopy and mass
spectrometry. By several periodic cultivations of the
mutant bacteriophages, it was shown that mutations of
this type are stably maintained during more than 50
generations. T4 related bacteriophages of the family
Myoviridae, for example, T-even, have the significant
homology amongst their genomes, which makes
possible to produce similar mutants. Thus, our method
was developed to obtain mutants with reduced
antigenicity which can be used for both the treatment of
systemic infections, and diarrhea in the case, when,
bacteriophages penetrate into the bloodstream. Such
phages can be used in medicine and veterinary. The
reported study was partially supported by RFBR,
research projects No. 13-04-00991, 16-44-230855
The dynamics of closed wood coniferous and deciduous forest formations for the last 15 years are presented
The analysis of territorial protection of the vegetation of natural zones, separate geobotanical allotments (formations), unique vegetative associations and Red Data Book species of vascular plants of Yakutia is given in the article. The objects of vegetation kingdom that need urgent actions for their habitats’ protection are marked
This article proposes the methods for the integrated analysis of riverside areas in Moscow. The most important results have been discussed
The article presents the researches of rats testicular morphology in oxidative stress induced by adapta-tion to low temperatures and its correction with Dihy-droquercetin. It was revealed that in oxidative stress Dihydroquercetin has protective properties prevent-ing spermatogenesis disorders and Leydig cells death in rat testis
Results of studying of meat efficiency of bull-calves of Kazakh white-headed, Kalmyk and Simmental breeds are evaluated. Features of formation of meat qualities of bull-calves in the conditions of the Bottom Volga region are considered