In cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
The article reports about the results of the new population of protected herb species studying in the Rostov region and in the Russian Federation - Cymbochasma borysthenica (Pall are given. ex Schlecht.) Klok. & Zoz (Orobanchaceae), registered in 2014 on the northern coast of Mius Liman in the Rostov-on-Don Region. The new habitat is located at considerable distance from the earlier known. The population area has about 200 sq. m, total number – more than 1000 uneven-age specimens of a seed and vegetative origin. Population consists of two cenopopulations one of which lives in community of transitional type from a timyannik to a stony steppe (association: Festuca valesiaca – Cleistogenes bulgarica – Cymbochasma borysthenica – Thymus dimorphus), another – as a part of the stony steppe (association: Festuca valesiaca + Agropyron pectinatum + Galatella villosa), created at the limestone shell rock exits. Cenopopulation in community of transitional type, despite the smaller area (50 sq. m), differs by more number and density. Both cenopopulations are similar in age ranges character and vitality of specimens. They belong to a normal type (capable to vegetative and seed self-maintenance) with a left-side age range that allows estimating them as rather stable, despite very limited area
The article deals with hawthorn Dagestan. It is noted
that the more than 100 species are found 14 species in
Dagestan. The most common of them are studied as a
source of biologically active substances and nutrients,
and they can be used as food additives for various
purposes. The content of biologically active substances
and nutrients are defined. The high content of phenolic
substances in C. pentagyna Waldst. et Kit, which play
an important role in the formation of immunity and
possessing P-vitamin activity is defined. Amino acid
and fatty acid composition of the fruit of the hawthorn
Crataegus pentagyna Waldst. et Kit, as the most
valuable for using as food additives is defined by ionexchange
and gas-liquid chromatography. Sixteen
amino acids were identified and quantified in the
protein component of hawthorn fruit. The high content
of essential amino acids such as lysine, threonine,
valine, methionine, leucine, isoleucine, phenylalanine.
The amount of essential amino acids is 296.1 mg / 100
g. Chromatographic separation of fatty acid methyl
esters has enabled us to establish the presence of the
following fatty acids: palmitic, oleic, stearic, linoleic
and linolenic, including established the presence of
essential fatty acids - the family of alpha-linolenic acid
OMEGA -3 and linoleic acid family of OMEGA -6
Dendroclimatic analysis of stands of radial increment of the natural forest and forest plantations of Scots pine in the Khrenovoe pine forest, growing in dry-moist site conditions is conducted. Cyclic dynamics of increment, weak expression of the low-frequency oscillation amplitude in the plantations stands is revealed. Direct correlation with precipitation has increased over the past 40 years, in complex suboron – to 0,48 – 0,50
The dependence of the grass cover state of the golf courses from the soil properties was observed. The soils named “replantozem” are the basic soil of gaming zone of "fairway". It is shown, that the heavy particle size structure with a predominance of clay particles is unfavorable to the lawn. During the golf course construction, natural soils were ameliorated with high sand content. This new made soils ensure the formation of better grass coverage despite the low humus content
The work presents the results of the scientific research in
the Taman Peninsula soil properties. We carry out the
historic review of the studies in the Taman Peninsula
soils, and its geological structure. We present
morphological characteristics of the soil profile for the
southern, calcareous, ultra heavy loam chernozem
developed from the loessial loams and forming genetic
horizons. The studies of the humus content in the soils
show the associations between its percentage and the
type and intensity of the agricultural use. Studies of the
soils structure and composition demonstrate marked
aggravation of the soils physical properties caused by
grape growing. The best results of the particle size
analysis have been achieved for the woodland soils. In
woodlands, after their establishment, the soils under the
grass and crown layers develop free of the
anthropogenic impact. We register self-supporting
growth of organic matter and natural processes of soil
fertility recovery; therefore, such soils are characterized
with a greater content of organic matter as compared to
the soils under grape plantings cultivated in the
monoculture regime. Reduced soil organic matter
(humus) content in vineyards results from the disturbed
biological interchange of the matter and increased
anthropogenic impact causing erosion processes
In the review, the stages of designing therapeutic cocktails of T4 type bacteriophages based on works by Harald Brüssow from Nestlé S.A. (Switzerland) are considered. The main stages of this process are identified: analysis of existing cocktails, selection of phages; creating a collection; cultivation of the host bacterium, multiplication of viruses; purification of the preparation; contamination testing; preservation, stabilization and storage; preclinical and clinical trials. H. Brüssow first studied the Russian drug " Coli-Proteus bacteriophage " of "Microgen" with the help of metagenomic analysis, electron microscopy and conducted its clinical studies. Prof. Brüssow considered the advantages of T4 bacteriophages for the treatment of Escherichia coli infections. Researchers studied methods of cultivation in Erlenmeyer flasks, in a bioreactor, in disposable sack cultivators for the propagation of viruses. For its purification the chromatography, centrifugation, filtration and polyethylene glycol precipitation were studied. To quickly check the contamination of phage cocktails, a mass spectrometry method is proposed. Researchers considered basic strategies, such as lyophilization, spray drying, the formation of microcrystals and microspheres to stabilize the preparations. They also reviewed the results of clinical trials of phage cocktails. We have listed the problems of selecting T4 bacteriophages from the point of view of modern knowledge. H. Brüssow and his colleagues carried out an interesting work on the construction of phage cocktails based on T4 type bacteriophages, and also revealed the problems of the current state of phage therapy
In this article we have carried out a comparative study of hydro-carbon-oxidizing activity of S-and R-morphological types of oil oxidizing Actinobacteria from the collection of Kuban State University. Signifi-cant differences observed in parameters of growth between S- and R-forms of oil oxidizing Actinobacte-ria. In S-forms it is higher than the maximum specific growth rate, which is typical for a wide range of hy-drocarbon degradation and a high degree of degrada-tion of pollutants. In experiments with the use of oil as a substrate and heavy oil S-forms, Actinobacteria quickly adapted to the environmental conditions
In this article there have been given an analysis of a complex of biochemical signs, usual for cold resistant apple, cherry and plum sorts. There have been found the regularities of variability for different complexes of biochemical signs which is similar for all investigated cultures
Gamma irradiation-inactivated cultural preparations (-IP) of African swine fever (ASF) non-haemadsorbing strains acquire a potential for haemadsorption induction in porcine bone marrow culture (PBMC). We have obtained some experimental data indicating that a modification of haemadsorption inhibition assay (HIA) which is an assay aimed at “removal” of γ -IP-induced haemadsorption may be used in research works to determine serospecificity rates of ASF virus non-haemadsorbing isolates