The results of the study presented showed that predator mite Ph. persimilis exhibited high food preference of the larval stage (30,2% of the total number consumed) compared to the egg (20,5%), nymph (13,4%) and adult (10,1%) stages of two spotted spider mite T. urticae. The functional response of Ph. persimilis feeding on eggs and adults of T. urticae was described by the Type II curve. At a maximum density of 60 prey per leaf disc, Ph. persimilis consumed an average of 22.8 eggs and 3.2 adults over an eight-hour period. Calculated time for handling of the prey by Ph. persimilis was on the average 0.074 hours (4.4 minutes) and 3.2 hours for T. urticae eggs and adults respectively. The mean number of T. urticae eggs consumed by Ph. persimilis progressively increased with the increase of the prey density, but the rate of predation (number of eggs consumed/prey density) decreased from 0.5 to 0.3 with the increasing of prey density. The mean number of adult T. urticae consumed by Ph. persimilis at densities of 20, 40 and 60 per leaf disk remained relatively constant. But the rate of predation decreased from 0.09 to 0.04 with increasing of prey density. The experiments showed that regardless of the strategy utilized by the predator it was the one that was efficient for the predator survival
In the article we have described the history of the studies and the modern features of fish community of the Black sea's river Durso (the length 14 km). It was established, that in present time the river includes only seven species of fishes from three ecological groups: freshwater (five species), saltwater (one specie) and marine (one specie). A bleack (Alburnus alburnus) and Colchis minnows (Phoxinus phoxinus colchicus) dominate by quantity in the structure of Durso river fish community, bleack and Prussian carp (Carassius auratus gibelio) dominate by biomass.
The negative changes of Durso river conditions were found in compare with the last years. First of all, it appears in biological pollution of fish species allochthonous for this river. In the period of the Durso river monitoring (1935–2014) it was established the distinct raise of allochthonous components with parallel reduce of variety of autochthonous fresh waters rheophilic species. The main reason is the building of reservoir in upper flow of the Durso river in 1976. The additional negative factor influence on of the Durso river fish community is the falling down of the water level during the important period of fish lifecycle. The best condition of fish community is in the middle part of the river were reaches combine with the cold springs and powerful flow
The article reports about the results of the new population of protected herb species studying in the Rostov region and in the Russian Federation - Cymbochasma borysthenica (Pall are given. ex Schlecht.) Klok. & Zoz (Orobanchaceae), registered in 2014 on the northern coast of Mius Liman in the Rostov-on-Don Region. The new habitat is located at considerable distance from the earlier known. The population area has about 200 sq. m, total number – more than 1000 uneven-age specimens of a seed and vegetative origin. Population consists of two cenopopulations one of which lives in community of transitional type from a timyannik to a stony steppe (association: Festuca valesiaca – Cleistogenes bulgarica – Cymbochasma borysthenica – Thymus dimorphus), another – as a part of the stony steppe (association: Festuca valesiaca + Agropyron pectinatum + Galatella villosa), created at the limestone shell rock exits. Cenopopulation in community of transitional type, despite the smaller area (50 sq. m), differs by more number and density. Both cenopopulations are similar in age ranges character and vitality of specimens. They belong to a normal type (capable to vegetative and seed self-maintenance) with a left-side age range that allows estimating them as rather stable, despite very limited area
Черешки листьев, взятых из растений ин витро, девяти межвидовых гибридов винограда: подвоев Берландиери х Рипариа ‘Кобер 5ББ’, Рипариа х Рупестрис ‘101-14’ и сортов (cvs): 'Бианка', 'Зигфридребе', 'Подарок Магарача’, ‘Первенец Магарача’, ‘Цитронный Магарача’, ‘Интервитис Магарача’ и гибридной формы ‘Магарач 100-74-1-5' культивировали на твердой среде NN, содержащей различные концентрации 2,4-D и BА в различных сочетаниях. Для того, чтобы инициировать рост клеточных суспензий, проэмбриогенные каллусы пересаживали в жидкую среду NN, дополненную 1.0 мг/л 2,4-Д и 0,2 мг/л BА. Субкультивирование этих суспензий в жидкую среду NN, дополненную 2 мг/л NAA и 0,1 мг/л BА, привело к развитию агрегатов эмбриоидов, а пересадка в жидкую среду с 0,5 мг/л BА вызвала развитие единичных глобулярных и сердцевидных эмбриоидов. Проэмбриогенные клеточные суспензии, состоящие из преимущественно отдельных клеток, могут быть использованы в трансформации генов и селекции на клеточном уровне с целью уменьшения вероятности появления химерных растений
In cell biology, actinomycin D is shown to have the ability to inhibit transcription. Actinomycin D does this by binding DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase. When soaking the seeds in a solution of actinomycin D, antibiotic blocks RNA synthesis and seed germination occurs at the expense of long-living RNA, available in the mature seed. In the article we present experimental data indicating that as storage seeds of winter barley are changing the action actinomycin D on the growth of coleoptiles. It is shown that actinomycin D at a concentration of 40 µg/ml in October reduced the growth, in December it was not authentically, and in February it was paradoxical amplified growing coleoptiles. We suggest the part of the actinomycin D fastened with growth inhibitor coleoptiles, whose structure changes during seed storage and increase the affinity of actinomicyn D to the inhibitors. At a concentration of 60 µg/ml actinomycin D effectively reduced of the coleoptiles growth. Suppression of seedlings roots growth was proportional to the concentration of the actinomycin D and exposure time
Development of plants in mixed or combined sowings is based on the symbiotic association in the basis of which we have decreased sexual process and its mechanism of genetic exchange among endosymbionts and stimulating of genetic exchange and expansion of the diversity of species among exosymbionts. The exception is the symbiosis of orchids and fungi - activators of orchids mycorrhiza with developed micellar system easily passing on saprophytic option. Coevolution of plant organisms with fungi indicates the specifics symbiogenic conjugate evolution, showing an example of the evolution of a small community with different contact with the external environment. The phenomenon of symbiogenesis has a long history and was manifested in a variety of different types of organisms. Today, some dependence of existence of one species from other organisms is observed at approximately 75% of higher plants and 90% of the animals in varying degrees of symbiogenic dependence from other species and taxa. Originality of symbiogenesis was analyzed by Academician A.A. Takhtadzhyan (1973) during its consideration of origin of eukaryotic cells as process of "assembly" of a complex system. Different approaches to determining the biological essence of symbiosis are known in the history of study of this phenomenon. On the whole phenomenon of symbiosis belongs to the category mutual relations of organisms of different phylogenetic groups
The problem of environmental contamination by heavy metals is significant problem of urbanization. The search of ways of indication heavy metals pollution becomes actual, because of their simplicity and affordability. A widely used test objects for heavy metals pollution indicators are leafy mosses used for bioindication, because they are highly sensitive to any stress factor. The research shows that bioindication with using leafy mosses as test objects is highly effective method definition of heavy metals pollution. Using of bioindication methods are promising techniques for the assessment of the contamination of ecosystems by heavy metals. Through the use of this method, it is possible indication of pollution of the surface layer of air with heavy metals. The epiphytic moss (Pylaisia polyantha) growing in different zones of the city of Rostov-on-Don, was used for the heavy metals pollution biomonitoring of urbosystems. The accumulation features of heavy metals in the epitaphic pylaisiella moss (Pylaisia polyantha) in the territory of the city of Rostov-on-Don have been considered. pylaisiella moss (P. polyantha) accumulates the largest amounts of the following heavy metals: Zn, Cr, Pb, Sr, Ni (Kc to 1.07), and Cu. According to the Kc values, all the studied elements accumulated by pylaisiella moss form the following series of biological uptake: Zn > Pb > Sr > Cr > V > Ni > Cu > Cо. The results of investigation showed that the epiphytic moss (Pylaisia polyantha) can be used as indicator of heavy metals pollution in different polluted zones
Green building, forestry, agriculture of the Rostov region is in desperate need of resistant and long-lived samples of deciduous trees of the first grade. The main object of the search of promising forms of deciduous trees were artificial plantations of Quercus robur in the Botanical Garden of SFU. The research identified several of samples of Quercus robur, which are of economic interest. Based on ecological and biological properties and economic qualities, the most promising on was a sample of Quercus robur var. tardiflora, planted in 1888. This sample is less receptive to pests and diseases. It also has a high winter hardiness and drought resistance and significantly superior to similar samples of Quercus robur along longevity and has a high decorative effect. Dedicated sample of Quercus robur var. tardiflora, and his generation from seed is very different from other samples of the population by time of phenological phases. The all main henological phases of this sample occured later by on 10-15 days. The sample belongs to the group of plants of late phenological dates start and end of the growing season. The phenology of dedicated samples contributes to their high steadiness into regional climate. It propagates by sowing freshly harvested seeds. Phenological features of the selected sample might be inherited in the seminal generations
The researches are devoted to the determination of physiological parameters of grafted apple-trees joining with the level of their resistance to the change of soil рН. The experiments were carried out in the conditions of lysimetric experience with the soils use characterizing by different value of рН. The scheme of experience included the following variants of soil medium reaction: neutral (рН 7,3), typical for black soils of plain part of the region (control); weak-acid (рН 6,2) existing in soils of foothill zone of the region (grey and brown forest); strong-acid (5.1) typical for brown forest soils; weak-alkaline(рН 8.3) occurring in black soils of south and sod-carbonate soils, strong-alkaline ( рН 8.8) typical for maternal types of many black soils. There were studied the varieties of apple-trees Prima, Florina and Renet Simirenko grafted on stocks M9 and MM106 and as well as corresponding the non-grafted stocks. The reaction of grafted apple-trees on change of рН of soil is specific and depends on adaptive possibilities of grafting components: varieties and stocks. At the stock M9 we can see the active growth of shoots and roots in the meaning of pH values from 5,1 (strong-acid reaction) to 7,3 (neutral). Plants MM106 prefer neutral (pH 7,3) and weak-alkaline (pH 8,3) soils. Independently from stocks for the variety Florina the most favorable weak-acid soils and the variety Prima – mainly neutral. The variety Renet Simirenko possesses enough high and stable functional activity in the conditions of wide range of the medium reaction: from very acid to alkaline one
Rendzina soils are very widespread in the Caucasus. Because of their ecological and genetic characteristics Rendzina has significant buffering capacity to chemical pollution. The object of investigation was calcareous leached soil. Location selection - Azishskaya ridge on the border of the Republic of Adygea and the Krasnodar region. As pollutants, we have selected Zn, Cd, Mo, Se, since soil contamination with these elements in the south of Russia is not uncommon. Contamination of zinc, cadmium, molybdenum and selenium causes deterioration in the biological properties of calcareous soils of the Western Caucasus. We have investigated the toxicity of the elements formed following series due to their influence on Rendzina soils: Zn> Se> Cd> = Mo. The study attempted to analyze the entire range of concentrations of the examined elements in the soil, currently occurring in nature. In most cases, all the investigated substances registered direct correlation between the concentration of the pollutant in the soil and the degree of reduction of biological indicators. The activity of catalase and dehydrogenase cellulolytic ability, plenty of bacteria of the genus Azotobacter, length of roots of radish can be used to monitor, diagnose and regulation of chemical pollution of soil Zn, Cd, Mo, Se