The article presents the results of the research of microflora composition of the leaf surface, the surface of fruit and flowers of Ribes nigrum, R. niveum, R . rubrum and Grossularia reclinata. The research was carried out in the seasonal dynamics from 2007 to 2014. The authors used the method of print for isolating microorganisms from plants. For convenience of calculations and contamination comparison of different environmental niches of plants the number of selected microorganisms was counted on the surface of 1 cm2. It was found out in the article that microorganisms on the surface of lamina are distributed unequally. The number of microorganisms on the bottom surface of the foliage in all periods turned out to be higher. The greatest number of microorganisms was recorded in autumn and reaches the highest value in October. The number of microorganisms on fruit surface increased with ripening and was the highest in July. The quantity on the flowers varies considerably throughout the flowering and is represented minimally compared to other plants niches. The study revealed species-specificity of microorganisms and the host plants. At the same time, long-term study of the microflora of plants belonging to one family made it possible to reveal species that are typical epiphytes
In the populations of Taxus baccata of Foothills and
Inside Mountain Dagestan, we have studied intra- and
interpopulation variability of morphological characters
of yew berry. The article has a contribution of
interpopulation differences in the feature of "diameter
of funnel" with the highest rates in Buynaksk
population and dependency of the form of yew berry
from this feature. According to the results of
discriminant analysis, we have revealed a distinction in
populations on this basis, although the classification
matrix has not given 100% of the forecast for any of
them. It is found, that the seeds of Khunzah population
have relatively large dimensions and yew berry has
more flattened shape compared to piedmont
populations, while the differences in populations based
on seeds are more pronounced than based on yew
berry at low volatility of the characteristics of the first
indicators. It is shown, that the characteristics of seeds
and yew berry of Khunzakh population indicate the
presence of certain genetic differences between
populations inside mountain and foothills, and that the
higher dissemination activity of first population is
aimed at self-preservation, due to the less favorable
conditions for the species. It is expected that the low
variability of the average values for yew berries of
cypress characteristics indicates the stability of the soil
and climatic conditions of its habitats
In the article we gave the review of the results of treatment of bronchitis of young large horned livestock with electro-reflex therapy. The results of the treatment with the clinical process of the illness and the results of biochemistry of blood were also discussed
The article presents results of the study of lavender reproduction (Lavandula angustifolia Mill) with methods of generative reproduction (seeds), vegetative reproduction (cutting, layening, divide of buch), clonal microreproduction using the culture in vitro.
The method of cloned microreproduction based on the culture of the isolated meristems which provides genetic identity of regenerated plants to initial forms and high coefficients of reproduction, improvement of landing material from fungal and bacterial infection, and also release from viruses at a combination with methods of thermotherapy and a chemotherapy is the most effective for the solution of objectives of seed farming of a lavender. As a material for carrying out researches we had plants of lavender (Lavandula angustifolia Mill), Sineva sorts, Stepnaya and perspective selection samples 337-9 and 310-17. Apical meristems 0,2-1,0 mm high isolated from top and axilary buds of a stalk of annual plants have been used as explant. Murasige and Skuga (MS) used as a basic nutrient medium for cultivation of the isolated meristems. Explants have been cultivated in the cultural room with 25-26 0C - temperature, lighting – 2-3 lx, relative humidity – 60-70%. It has been established that both April and October are the best month for isolation of meristems correspond calendar to phases of spring and autumn growth at the lavender donor plants and that optimum is an agar nutrient medium of MS, added with kinetin (1,0 mg/l) and GC (1,0 mg/l); frequency of regeneration of all studied genotypes was 90,0-100,0 %. Feature of morphogenesis of lavender meristems in vitro culture was already at the first stage of clonal microreproduction as there was a multiple shoot formation. Studying of features of development of lavender microplants during ten passages also was carried out, as the level of stability of regeneration processes throughout several cycles of a miсrograftage is one of important factors on which efficiency of microreproduction depends. We have also shown, that the efficiency of reproduction remains at the stable level of the sort named Sineva and sample #337-9 to the 8th passage (1:7,77-12,45 and 1:7,60-11,85 respectively), at the variety called Stepnaya - to the 7th passage (1:6,10-11,81), at the sample #310-17 - to the 6th passage (1:6,17-8,37)
Toxicity complex compost arises during compiling of organomineral mixtures of various waste of life, agriculture and mineral industries. One of detoxification factors of complex compost is the ability of heavy metal cations to the formation insoluble compounds, which are fixed by clay materials and different disperse systems, and differ markedly by calcium content, acidity and humus soil-absorbing complex
The article presents the materials of the study of the
features of cultivation of Lactobacillus sp. on
different culture medium. We have made a
comparison of standard culture medium for
cultivation Lactobacillus sp. and determined the
most optimal culture medium
The article presents the structural and phylogenetic
analysis of VIT_02s0033g00410,
VIT_02s0033g00390 and VIT_02s0033g00450
genes of the grapevine genome and the closely
related orthologous gene MYB114 of the
Arabidopsis genome. These genes are responsible
for the biosynthesis of anthocyanin in the organs of
model plants and are of interest not only for practical
production and breeding, but also for fundamental
research. These genes were analyzed for GCcomposition
of nucleotides, the presence of cisregulatory
elements and promoter regions. DNA and
protein sequences were aligned to look for similar
elements, which allowed further analysis of the
ultraconservative domains of four genes. Based on
the results of search and identification of the
conservative regions, a cluster tree was constructed,
which made it possible to identify the separation of
gene sidelines from, presumably, the main one. At
the same time, the construction of consensus trees
based on DNA and protein sequences revealed their
absolute similarity. "The Minimal Evolution Tree"
allowed calculating the approximate dates of the
appearance of the mutations and the divergence
times of the gene branches between each other. At
the same time, the appearance of the Vitis genus and
its separation from the Rosales was taken as the time
first divergence point. In the end, homologous
metabolic pathways were searched between
grapevine and Arabidopsis, which revealed the
presence of homologous proteins in the grape
proteome. In this turn, it already confirms the
existence of similar biosynthetic pathways and, as a
consequence, interactions such as "DNA-protein"
and "protein-protein"
In this article there have been given an analysis of a complex of biochemical signs, usual for cold resistant apple, cherry and plum sorts. There have been found the regularities of variability for different complexes of biochemical signs which is similar for all investigated cultures
THE ROLE OF MINERAL FERTILIZERS IN REGULATION OF HARMFUL ENTOMOFAUNAS ON PERENNIAL LEGUME SEED CROPS
The article presents the results of the studies on the effect of phosphorus-potassium fertilizers on the number of main pests of perennial legumes by phases of their development in the conditions of Central Black-Soil Region southeast
Differentiation of temperature influence on embryos at incubation of eggs shows positive results: the output of chickens is synchronized, duration of embryogenesis is reduced, deductibility of eggs raises, the young growth output increases