DETECTION OF SIBERIAN STURGEON HERPESVIRUS GENOME BY REAL-TIME PCR AND ITS APPLICATION FOR FISH FARM
This article describes the research to develop a test system for the diagnosis of Siberian sturgeon herpes virus PCR in real time and its possible use in the examination of fish farms
The research of the territory adjacent to Novocherkassk state district power station, for the content of polycyclic aromatic hydrocarbons (on an example of benzo(a)pyrene) is presented. The benzo(a)pyrene content in soils has been determined with a method of subcritical water extraction. The content of benzo(a)pyrene in soils of the territory adjacent to Novocherkassk state district power station has been estimated. It is shown, that contents of benzo(a)pyrene excess over maximum permissible concentration in soils of five-kilometer zone around the companies
This article summarizes the results of the study of physical, chemical and biological characteristics of mineral waste; it analyzes the specific features of its behavior when applied to the soil in pure form and in complex compost; the possibility of using some of them as an ameliorant for improving the fertility of soil common in the steppe zone of the region
The research work was conducted at the Research Laboratory
of Biotechnology, Biochemistry, Biophysics
Department of Kuban State Agrarian University. The
main purpose of this study was to select the most productive
type of fungus of a genus of Trichoderma and
optimal nutrient medium on basis of a waste of reprocessing
of soy for acquire of protein enzyme feed supplement.
For selection of a fungus of a genus
Trichoderma used 3 types: Trichoderma viride,
Trichoderma lignorum, and Trichoderma harsianum.
We used 3 types of nutrient media as a carrier for
micromycete, which were based on soy okara and the
additional sources of reprocessing of raw plant material
– husk of a sunflower, husk of wheat (bran) and
husk of rice. The results demonstrated the benefits of
using the nutrient medium based on soy okara forms
and husk of a sunflower fungus to enhance
Trichoderma lignorum production
The results of studies to determine growth-promoting activity and the effect on seed quality of fall wheat of perspective bacterial strains of antagonistic Fusarium pathogens are presented. We have selected the active strains perspective for the development of biopreparations, based on their type of multifunctional action
The article presents the ecological characteristics of soil types and forest cover of the Red October forestry of the State Unitary Enterprise of the Republic of Adygea. We have defined their relationship and the division into groups of forestry and environmental values. A complex combination of bioclimatic factors, parent rocks and topography has led to a large diversity of soil cover. We have revealed that different soils have different degrees of erosion on sloping hillsides, ravines, and steep slopes are dominated by strongly eroded soils. The most common form of physical degradation of soils of the red October experienced forestry is seal root layer observed on heavily used areas and pastures. Also, we found that poorly structured soils containing little humus are prone to compaction. Methods of rooting out areas after clear cutting of forests lead to secondary soil degradation. It especially enhanced if, at the moment of stressing, the soil is in the waterlogged condition. Therefore, at reforestation we should use different reclamation processing. And this leads to the difficulty of their use in reforestation. It is established that intensive anthropogenic activity leads to degradation of soil and forest cover of the study area
This review is devoted to the 60 anniversary of the
winter wheat variety (Triticum aestivum L.)
Bezostaya 1 – a masterpiece of world selection. This
variety played a great role in increasing total grain
yields in the Kuban Region. It also was an important
gene source for Russian and world breeding. This
variety has initiated and was for many years a party
to research molecular biological mechanisms of
wheat frost resistance formation. The article
summarizes data about the peculiarities of
functioning of protein-synthesizing system it sprouts
(coleoptilies) under the influence of hardening
temperature (4oC): translational activity in vitro,
poliribosomts, degree of polyadenilation and stability
of total mRNA and specific mRNA translational
elongation factor α subunit 1 (eEF-1 α). The in vitro
(ommp) system, which adequately characterizes the
relative mRNA stability in vivo was used for these
measurements. The effects of cooling and light on
mRNA stability were shown to be cultivar-specific.
The hardiness of winter wheat has a positive
relationship with the amount of RNA and DNA, and
a negative correlation with the amount of magnesium
cations in mature grain that can serve as a simple
marker of frost resistance. The main feature of the
variety Bezostaya 1 is high content of magnesium
ions (Mg++) in RNA molecules and relatively low
variation of Mg++-depending molecularphysiological
responses for different environmental
conditions. The knowledge gained in the study of
variety of Bezostaya 1 contributes to the
understanding of molecular biological processes that
underlie the selection and determining its future
success
The article considers the new method for recovery of P. arbelaeziion intact material on the basis of grafting into endogenous pathogens resistant Passiflora species in vivo. The influence of the explants origin depending on the stock of intact plants on the development of regenerated P. arbelaezii under in vitro condition is examined
This article presents the results of researching impact of contaminant absorption with pathological state of tree species in extreme condition of Voronezh reservoir near-shore
The article reveals historical aspect of the Donets
Ridge petrophytic vegetation study. The stages of
formation of concepts and terms in the typology of
vegetation of the rocky outcrops of the steppe zone