In the process of the research we had accomplished an analysis of allelic polymorphism of self-incompatibility in pears in demand in the production and breeding of modern varieties of pears of North-Caucasian Zonal Research Institute of Horticulture and Viticulture collection of genetic resources. In the first stage the consensus primers PycomC1F and PycomC5R were used. With obtained data, after identification of the alleles studied in varieties using consensus primers an allele specific S5 and S8 DNA markers were used for confirming the presence / absence of data allele studied cultivars
Study results of Crambe maritima L. growth and development at introduction in Botanical garden of SFU are presented in article. It was revealed that the life cycle of majority specimens lasts three years. Results the ontogenetic and phenologic studies testify about success of primary introduction of Crambe maritima
Creation of complex compost provides the inclusion of about 10 kinds of organic and mineral wastes, the management of which optimizes their unification in order to create effective compounds, which noticeably change the physical, chemical and biological complexes by enhancing their long activity (4-6 years), ranging from their introduction into the upper layer of the soil, taking into account the development of microorganisms, strengthening water-physical links, enriching chemical ones and extending biological relationships with the expansion of ecological niches
The article considers the enzymatic activity and some other ecological and biological properties of zonal soils of the Crimea (cambisol, Chromic cambisol, different subtypes of chernozems). We have revealed significant differences in catalase, dehydrogenase, polyphenol oxidase, peroxidase,
invertase for soils of the Crimea, which can not be explained only by the content of soil organic matter. Despite the low humus content of the soil, some have a high level of some enzymes. The level of enzyme activity depends on the reaction medium, the content of carbonate and other soil properties. We have also revealed that the agricultural use of brown
soils under vineyards leads to a significant change in their properties and enzymatic activity
In order to identify high-genotypes in natural stands of
Sacutifolia Willd we worked to identify the categories of breeding trees. Experimentally proved, that for breeding to increase the productivity of bark of Salixacutifolia Willd the selection would be best carried out with plants, the diameter of the barrel of which is in the range of 9 to 16 cm, tree age 15 - 19 years
The article provides the data of studying the biological
properties of Newcastle disease virus isolates
NDV/Pigeon/Rus/Kursk/1/13 and NDV/Pigeon/Rus/Kursk/2/13 derived from pigeons in pigeon houses in the Kursk region in 2013 using virological and molecular genetic methods
Human-induced disturbance on water objects was appraised with chemo and biological methods, the results of which are turned out to be comparable
This article presents the data of chorological analysis of the flies’ fauna (Insecta, Diptera) of the Kamishanova Polyana Nature reserve. The analysis of distribution of 432 species of the reserve allowed to identify the 4 groups and 16 types of areals
The article provides an overview of the results of experimental works of the authors on the use of different methods of fractionation of Lucerne juice to obtain feed additives
Черешки листьев, взятых из растений ин витро, девяти межвидовых гибридов винограда: подвоев Берландиери х Рипариа ‘Кобер 5ББ’, Рипариа х Рупестрис ‘101-14’ и сортов (cvs): 'Бианка', 'Зигфридребе', 'Подарок Магарача’, ‘Первенец Магарача’, ‘Цитронный Магарача’, ‘Интервитис Магарача’ и гибридной формы ‘Магарач 100-74-1-5' культивировали на твердой среде NN, содержащей различные концентрации 2,4-D и BА в различных сочетаниях. Для того, чтобы инициировать рост клеточных суспензий, проэмбриогенные каллусы пересаживали в жидкую среду NN, дополненную 1.0 мг/л 2,4-Д и 0,2 мг/л BА. Субкультивирование этих суспензий в жидкую среду NN, дополненную 2 мг/л NAA и 0,1 мг/л BА, привело к развитию агрегатов эмбриоидов, а пересадка в жидкую среду с 0,5 мг/л BА вызвала развитие единичных глобулярных и сердцевидных эмбриоидов. Проэмбриогенные клеточные суспензии, состоящие из преимущественно отдельных клеток, могут быть использованы в трансформации генов и селекции на клеточном уровне с целью уменьшения вероятности появления химерных растений