Scientific Journal of KubSAU

The article presents results of the study of lavender reproduction (Lavandula angustifolia Mill) with methods of generative reproduction (seeds), vegetative reproduction (cutting, layening, divide of buch), clonal microreproduction using the culture in vitro. The method of cloned microreproduction based on the culture of the isolated meristems which provides genetic identity of regenerated plants to initial forms and high coefficients of reproduction, improvement of landing material from fungal and bacterial infection, and also release from viruses at a combination with methods of thermotherapy and a chemotherapy is the most effective for the solution of objectives of seed farming of a lavender. As a material for carrying out researches we had plants of lavender (Lavandula angustifolia Mill), Sineva sorts, Stepnaya and perspective selection samples 337-9 and 310-17. Apical meristems 0,2-1,0 mm high isolated from top and axilary buds of a stalk of annual plants have been used as explant. Murasige and Skuga (MS) used as a basic nutrient medium for cultivation of the isolated meristems. Explants have been cultivated in the cultural room with 25-26 0C - temperature, lighting – 2-3 lx, relative humidity – 60-70%. It has been established that both April and October are the best month for isolation of meristems correspond calendar to phases of spring and autumn growth at the lavender donor plants and that optimum is an agar nutrient medium of MS, added with kinetin (1,0 mg/l) and GC (1,0 mg/l); frequency of regeneration of all studied genotypes was 90,0-100,0 %. Feature of morphogenesis of lavender meristems in vitro culture was already at the first stage of clonal microreproduction as there was a multiple shoot formation. Studying of features of development of lavender microplants during ten passages also was carried out, as the level of stability of regeneration processes throughout several cycles of a miсrograftage is one of important factors on which efficiency of microreproduction depends. We have also shown, that the efficiency of reproduction remains at the stable level of the sort named Sineva and sample #337-9 to the 8th passage (1:7,77-12,45 and 1:7,60-11,85 respectively), at the variety called Stepnaya - to the 7th passage (1:6,10-11,81), at the sample #310-17 - to the 6th passage (1:6,17-8,37)

The article presents the researches of rats testicular morphology in oxidative stress induced by adapta-tion to low temperatures and its correction with Dihy-droquercetin. It was revealed that in oxidative stress Dihydroquercetin has protective properties prevent-ing spermatogenesis disorders and Leydig cells death in rat testis

The research results on regeneration activity of wilding cuttings of phylloxeraresistant varieties of grapes influenced by heteroauxin treatment are analysed in the article. The preliminary studies were held in All-Russian research institute of viticulture and wine-making of Ya.I. Potapenko using cuttings of 3 grape varieties – Berlandieri x Riparia Cober 5bb, Riparia x Rupestris 101-14 and Berlandieri x Riparia CO-4. The cuttings were soaked during 48 hours in 0,005 % heteroauxin and were implanted in sand in adjustable conditions of temperature, humidity and aeration. In 2012-2013 experiments were continued in Kuban State Agrarian University. The varieties Gravesak, RSB and Berlandieri x Riparia Teleki 5C were added to the experiment. Three-items buds were shooted in damp steamed sawdust in a film hothouse. In preliminary researches application of heteroauxin has allowed to increase root-growing by 20,3-40,0 %. The greatest increase of root-growing (40 %) was observed on variety СО-4, that is characterized by the weakest potential rhizogenesis activity, and the least (20,3 %) on control variety Cober 5 bb. Next researches show that treatment of cuttings basal ends by 0,01 % heteroauxin activated their regeneration process, that was expressed in: increase in sprouts length – variety 5 C, 101-14 and Gravesak by 23,1- 157,6 %; increasing of cuttings root-growing from 16,7 % on variety 5C to 60,0 % on variety Cober 5 bb, the quantity of cuttings with not fewer of 3 roots from accordingly 13,0 % to 54,7 %; increasing of roots from 0,7 on variety СО-4 to 2,7 on variety 101-14 or in 1,7-2,7 times. The application of heteroauxin on variety RSB did not show positive results, because of the variety peculiarities

In the present article the authors provide an overview of the results of experimental works for the use of electro activated water solutions in agroindustrial complex

In the article we recommend 7 species of Stratiomyidae to be used as umbrella and conserved species for 5 landscape complexes of the North-west Caucasus and 4 species for 4 landscape complexes of the Crimea

The efficiency of using of organic iodine and selenium as a part of a new “IODDAR-Zn” fodder additive and DAFS-25 preparation in goats feeding from the point of view of increasing quality of milk and dairy products is proved in the article. The technology of production of new dry sour-milk bioproduct from the goat milk, intended for children, is presented

In the Southern Federal University on the genetic basis of sunflower inbred line 3629, a collection of plastid mutants with varying degrees of chlorophyll deficiency was created by inducing Nnitrosomethylurea. Chlorophyll content was associated with their photosynthetic activity. It was found that the lower the chlorophylls content in mutant plastids, the lower the sugar concentration in plant tissues. For example, during the entire period of plant growth for whites (1.0-3.0 % chlorophylls a+b from control) var- 10, var-17 and yellows (6.0-9.5 % chlorophylls a+b from control) var-29, var-33 leaf areas of variegated mutants depending on the development phase and the content of green pigments are characterized by a low (2-7 fold) sugar content. Yellow-green (75.5% chlorophylls a + b from control) leaves of en:chlorina- 7 contain a higher level of carbohydrates, although it is 1.5-2 fold lower than at line 3629. Monosaccharides are products of hydrolytic reactions catalyzed by β- glycosidases. It was shown, that the activity level of β- galactosidase and β-glucosidase exceeded the corresponding indicators of the control at 1.5-2 and 2-7 fold, respectively. Similarly to enzymes from the water-soluble fraction, membrane-bound β- glycosidases also showed increased activity in the leaves of the investigated mutants, compared to the control green plants of 3629. Consequently, the activity of β-glycosidases increases dramatically in leaf tissues with deficiency of photosynthetic. Thus, chlorophyll mutations can lead to a change in the expression of nuclear genes, resulting in a significant increase in the activity of β-glycosidases in the mutant organelles themselves

The article presents the violation of water protection zones. The change in soil fertility. Transparency is linked to the nature of the coating beaches and surface destruction. Surface runoff depends on the vegetation cover. The destruction of vegetation (deforestation, excessive grazing, fire), incorrect plowing surface (along the slope) and tillage farming without following the rules lead to increased erosion, local soil erosion, gully erosion and the rise to an increase in turbidity of rivers. Environmental effectiveness of forest plantations. Prevention of pollution and siltation of river. Improving the sanitary condition of the territory

Complicated compost with organic and mineral components, has a serious impact on the physical, chemical and biological properties of topsoil significantly modifies its cycles of elements and substances; with the Carboniferous and up to now it has been established the supremacy of the recovery processes in landscapes as compared to oxidation processes through the introduction of complicated compost in their stabilizing factors

The article presents the results of the studies on the effect of phosphorus-potassium fertilizers on the number of main pests of perennial legumes by phases of their development in the conditions of Central Black-Soil Region southeast