Scientific Journal of KubSAU

Study of dynamic sensitive of microorganisms shows wave shape change of characters with change of dominance with sensitive aminoglicozids and hinolone ftorhinolone groups of drugs

There are numerous reports about the possibility of sensitization to tuberculin animals when infected with pectoris and Rhodococcus and it is considered to be expedient to create one monoallergenov to differentiate allergic reactions. In this context, the spread of pectoris and Rhodococcus in nature, the general physico-chemical and biological properties of mycobacteria, increasingly emerging reports of a possible sensitization of the microorganism, require a detailed study of them in order to determine the specificity of allergy

In medical practice, RLA as the alarm express train - test for revealing antigenes of microorganisms is convenient at use in diagnostic laboratories, and also at carrying out of mass inspections. To advantages of RLA, as a method of serologic diagnostics of bacterial and virus infections, it is possible to attribute the following moments: high specificity and sensitivity; absence of necessity for the complex equipment for statement and reactions and registration of results; data up to a minimum of quantity of components of reaction; an opportunity of reception of inert carriers with the set characteristics; an opportunity of reception of inert carriers with the set characteristics

In this article, the authors have defined the acute toxicity of Roksatsin preparation, which represents a 20 % aqueous solution of polyhexamethylene guanidinehydrochloride (PHMG). The acute toxicity of Roksatsin in the first series of experiments was studied on white mice upon intragastric administration. In the second series of the experiments, Roksatsin was administered subcutaneously to white rats and in the third series of experiments the preparation was administered intracisternally to cows. The results of the first series of experiments showed that Roksatsin by the internal method of appointment is slightly toxic to white mice. In this experiment not been a single case of acute intoxication and death of animals. Experiments to determine the acute toxicity of the preparation for white rats were performed in triplicate. In the first series of experiment, the LD50 was 4.8 ml/kg. It is found, that the calculated coefficients LD50 for other two series were close to the first one: the second - 4.2 ml/kg and the third - 4.5 ml/kg. Therefore, the authors found that the Roksatsin preparation upon subcutaneously administration according GOST 12.1.007-76 is a low-toxic compound (4th class of danger). As a result of the third series of the experiments, it was found that the intracisternally administration of Roksatsin preparation to cows in a dose of 5 ml is not toxic to the animal organism. Intracisternal administration of Roksatsin does not have a significant impact on the morphological and biochemical indices of blood. On this basis, Roksatsin preparation with different routes of administration is low-toxic and it can be recommended for clinical researches

The diagnostic preparations created on the basis of polymeric carriers, find wide application in diagnostics of various diseases with the purpose of detection of small quantity of antibodies or antigenes in various substrata. Due to the physical and chemical properties, polymeric particles are convenient carriers at creation of various diagnostic tests - systems in area immunoassay. It is possible to attribute the following moments to advantages of reaction of latex - agglutination: simplicity and speed of performance, absence of necessity for the complex equipment, reproducibility and accuracy, an opportunity of reception of the big parties of standard and homogeneous polymeric suspensions

The influence of the complex alumosilicate prepara-tion of Silimix on productivity, rates of red blood cells and certain microcells in blood serum against nutri-tional anemia of calves is studied. The increase in additional weights of calves and antianemic effect of the preparation is established

Efficiency of correction of hepatic insufficiency of dogs by means of a medicinal solution on a basis of cluster silver and bile is shown. After application of therapy for the ill dogs, it was marked: restoration of the clinical status, morbidity disappearance in belly area and yellowness of mucous membranes for 9 days. For 19 days biochemical indicators of blood reached level of healthy dogs

The "multiplex" PCR technique in a combination with automatic extraction of nucleonic acids allows to carry out the control of activators of sharp intestinal infections of the bacterial and virus nature over objects of an environment with high speed, specificity and sensitivity

The opportunity of revealing of salmonellas of group И with immunochromatography method in foodstuff, water and air is shown

In the article, the effects of approbation of complex therapy are resulted at oligotrophy of calves with use of the preparation of protein hydrolysis and Dipromo-nium - M. The antihypotrophic action of preparations and efficiency of the scheme for augmentation of additional weights of calves and optimization of bio-chemical profile of blood are established