Name
Aniskina Mariya Vladimirovna
Scholastic degree
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Academic rank
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Honorary rank
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Organization, job position
Kuban State Agrarian University
Web site url
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Articles count: 4
The work was carried out in the scientific-research
laboratory of the chair of Biotechnology, Biochemistry
and Biophysics of Kuban SAU, the aim of which is the
search of optimal conditions of obtaining the
hydrolyzates of lactic acid bacteria by thermoacid and
enzymatic ways. The pure cultures of microflora of
gastrointestinal tract of quails − Lactobacillus agilis,
Lactobacillus intermedius and Lactobacillus salivarius
were the object of researches. Under conducting the
thermal acid hydrolysis there were used the same
correlations of bacterial mass and water as well as
procedures of clearing of hydrolyzates. The variable
conditions were рН, temperature and time of hydrolysis.
When carrying out the enzymatic hydrolysis in this
study there was used the enzyme from the group of
muramidase lysozyme. The amount of enzymes and
time of carried out hydrolysis were the variable
conditions under obtaining the enzymatic
hydrolyzates. In the process of researches there were
studied: qualitative content of total protein, peptides
with М.м. < 1500, protein and peptides with M.m. >
1500, aminoacids and as well as GMDP
(glucosaminilmuramilpentapeptide). On the basis of
carried out experiments there was stated that according
to all components of biologically active substances the
thermal acid way is more effective than enzymatic. The maximum accumulation of BAA in hydrolyzates
by different ways was revealed at destruction of cells
Lb. intermedius. Content of GMDP in thermal acid
hydrolyzate was higher than in enzymatic on
0,028 g/100 ml (51,8 %)
The article presents materials about selection and
optimization of the nutrient medium for cultivation
of Propionibacterium shermanii. We have shown a
high positive effect expressed in the intensive
growth of microorganisms of Propionibacterium
shermanii on optimized medium with the juice
from the tomatoes, as well as replacement of
glucose on corn extract, which allows concluding
that these components have a positive impact on
the accumulation of biomass of Propionibacterium
shermanii
The article presents the materials of the study of the
features of cultivation of Lactobacillus sp. on
different culture medium. We have made a
comparison of standard culture medium for
cultivation Lactobacillus sp. and determined the
most optimal culture medium
We have presented materials about nutrient media
for growing and industrial the cultivation of
Propionibacterium shermanii: Trypticase Yeast
Extract Glucose Medium, the culture medium
Ellinger and ATCC Medium 33. We have studied pH
change and its impact on the biomass accumulation
of propionic acid microorganisms. We have also
defined the best time of cultivation